{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:18:36Z","timestamp":1791861516945,"version":"4.3.4"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Genomics"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>The complex genome of rapeseed (<jats:italic>Brassica napus<\/jats:italic>) is not well understood despite the economic importance of the species. Good knowledge of sequence variation is needed for genetics approaches and breeding purposes. We used a diversity set of <jats:italic>B. napus<\/jats:italic> representing eight different germplasm types to sequence genome-wide distributed restriction-site associated DNA (RAD) fragments for polymorphism detection and genotyping.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>More than 113,000 RAD clusters with more than 20,000 single nucleotide polymorphisms (SNPs) and 125 insertions\/deletions were detected and characterized. About one third of the RAD clusters and polymorphisms mapped to the <jats:italic>Brassica rapa<\/jats:italic> reference sequence. An even distribution of RAD clusters and polymorphisms was observed across the <jats:italic>B. rapa<\/jats:italic> chromosomes, which suggests that there might be an equal distribution over the <jats:italic>Brassica oleracea<\/jats:italic> chromosomes, too. The representation of Gene Ontology (GO) terms for unigenes with RAD clusters and polymorphisms revealed no signature of selection with respect to the distribution of polymorphisms within genes belonging to a specific GO category.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Considering the decreasing costs for next-generation sequencing, the results of our study suggest that RAD sequencing is not only a simple and cost-effective method for high-density polymorphism detection but also an alternative to SNP genotyping from transcriptome sequencing or SNP arrays, even for species with complex genomes such as <jats:italic>B. napus<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-13-281","type":"journal-article","created":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T09:15:34Z","timestamp":1347700534000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":115,"title":["High-throughput polymorphism detection and genotyping in Brassica napus using next-generation RAD sequencing"],"prefix":"10.1186","volume":"13","author":[{"given":"Anja","family":"Bus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jochen","family":"Hecht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Huettel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Reinhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Stich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,6,24]]},"reference":[{"key":"4156_CR1","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1007\/BF02672069","volume":"9","author":"K Arumuganathan","year":"1991","unstructured":"Arumuganathan K, Earle ED: Nuclear DNA content of some important plant species. 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