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Understanding the spatial transition of microbiota from normal oral cavities through the infected root canal to the acute periapical abscess can improve our knowledge of the pathogenesis of endodontic infections and lead to more effective treatment. We obtained samples from the oral cavity, infected root canal and periapical abscess of 8 patients (5 with localized and 3 with systemic infections). Microbial populations in these samples were analyzed using next-generation sequencing of 16S rRNA amplicons. Bioinformatics tools and statistical tests with rigorous criteria were used to elucidate the spatial transition of the microbiota from normal to diseased sites.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>On average, 10,000 partial 16S rRNA gene sequences were obtained from each sample. All sequences fell into 11 different bacterial phyla. The microbial diversity in root canal and abscess samples was significantly lower than in the oral samples. <jats:italic>Streptococcus<\/jats:italic> was the most abundant genus in oral cavities while <jats:italic>Prevotella<\/jats:italic> and <jats:italic>Fusobacterium<\/jats:italic> were most abundant in diseased samples. The microbiota community structures of root canal and abscess samples were, however, more similar to each other than to the oral cavity microbiota. Using rigorous criteria and novel bioinformatics tools, we found that <jats:italic>Granulicatella adiacens, Eubacterium yurii, Prevotella melaninogenica, Prevotella salivae, Streptococcus mitis,<\/jats:italic> and <jats:italic>Atopobium rimae<\/jats:italic> were over-represented in diseased samples.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>We used a novel approach and high-throughput methodologies to characterize the microbiota associated normal and diseased oral sites in the same individuals.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-13-345","type":"journal-article","created":{"date-parts":[[2012,7,28]],"date-time":"2012-07-28T14:14:19Z","timestamp":1343484859000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":136,"title":["Microbial transformation from normal oral microbiota to acute endodontic infections"],"prefix":"10.1186","volume":"13","author":[{"given":"William W L","family":"Hsiao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin L","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenqiu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheron","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire M","family":"Fraser-Liggett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf F","family":"Fouad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,7,28]]},"reference":[{"key":"4128_CR1","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1111\/j.1600-9657.1992.tb00249.x","volume":"8","author":"M Eckerbom","year":"1992","unstructured":"Eckerbom M, Magnusson T, Martinsson T: Reasons for and incidence of tooth mortality in a Swedish population. 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