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The present study uses a transcriptomic approach to characterize the gene expression profile from the non-stimulated venom gland of <jats:italic>Tityus stigmurus<\/jats:italic> scorpion.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>A cDNA library was constructed and 540 clones were sequenced and grouped into 153 clusters, with one or more ESTs (expressed sequence tags). Forty-one percent of ESTs belong to recognized toxin-coding sequences, with transcripts encoding antimicrobial toxins (AMP-like) being the most abundant, followed by alfa KTx- like, beta KTx-like, beta NaTx-like and alfa NaTx-like. Our analysis indicated that 34% of the transcripts encode \u201cother possible venom molecules\u201d, which correspond to anionic peptides, hypothetical secreted peptides, metalloproteinases, cystein-rich peptides and lectins. Fifteen percent of ESTs are similar to cellular transcripts. Sequences without good matches corresponded to 11%.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This investigation provides the first global view of gene expression of the venom gland from <jats:italic>Tityus stigmurus<\/jats:italic> under resting conditions. This approach enables characterization of a large number of venom gland component molecules, which belong either to known or non yet described types of venom peptides and proteins from the Buthidae family.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-13-362","type":"journal-article","created":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T12:15:07Z","timestamp":1343823307000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Profiling the resting venom gland of the scorpion Tityus stigmurus through a transcriptomic survey"],"prefix":"10.1186","volume":"13","author":[{"given":"Diego D","family":"Almeida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katia C","family":"Scortecci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo S","family":"Kobashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucymara F","family":"Agnez-Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia R B","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arn\u00f3bio A","family":"Silva-Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00e1cio de L M","family":"Junqueira-de-Azevedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus de F","family":"Fernandes-Pedrosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,8,1]]},"reference":[{"key":"4187_CR1","volume-title":"The biology of scorpions","author":"GA Polis","year":"1990","unstructured":"Polis GA: The biology of scorpions. 1990, Stanford University Press, California"},{"key":"4187_CR2","volume-title":"Book Manual de Diagn\u00f3stico e Tratamento de Acidentes por Animais Pe\u00e7onhentos","year":"2001","unstructured":"Book Manual de Diagn\u00f3stico e Tratamento de Acidentes por Animais Pe\u00e7onhentos. 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