{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:33:32Z","timestamp":1761708812678},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Genomics"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Secretoglobin 1A1 (SCGB 1A1), also called Clara cell secretory protein, is the most abundantly secreted protein of the airway. The <jats:italic>SCGB1A1<\/jats:italic> gene has been characterized in mammals as a single copy in the genome. However, analysis of the equine genome suggested that horses might have multiple <jats:italic>SCGB1A1<\/jats:italic> gene copies. Non-ciliated lung epithelial cells produce SCGB 1A1 during inhalation of noxious substances to counter airway inflammation. Airway fluid and lung tissue of horses with recurrent airway obstruction (RAO), a chronic inflammatory lung disease affecting mature horses similar to environmentally induced asthma of humans, have reduced total SCGB 1A1 concentration. Herein, we investigated whether horses have distinct expressed <jats:italic>SCGB1A1<\/jats:italic> genes; whether the transcripts are differentially expressed in tissues and in inflammatory lung disease; and whether there is cell specific protein expression in tissues.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We identified three <jats:italic>SCGB1A1<\/jats:italic> gene copies on equine chromosome 12, contained within a 512-kilobase region. Bioinformatic analysis showed that <jats:italic>SCGB1A1<\/jats:italic> genes differ from each other by 8 to 10 nucleotides, and that they code for different proteins. Transcripts were detected for <jats:italic>SCGB1A1<\/jats:italic> and <jats:italic>SCGB1A1A<\/jats:italic>, but not for <jats:italic>SCGB1A1P<\/jats:italic>. The <jats:italic>SCGB1A1P<\/jats:italic> gene had most inter-individual variability and contained a non-sense mutation in many animals, suggesting that <jats:italic>SCGB1A1P<\/jats:italic> has evolved into a pseudogene. Analysis of <jats:italic>SCGB1A1<\/jats:italic> and <jats:italic>SCGB1A1A<\/jats:italic> sequences by endpoint-limiting dilution PCR identified a consistent difference affecting 3\u2009bp within exon 2, which served as a gene-specific \u201csignature\u201d. Assessment of gene- and organ-specific expression by semiquantitative RT-PCR of 33 tissues showed strong expression of <jats:italic>SCGB1A1<\/jats:italic> and <jats:italic>SCGB1A1A<\/jats:italic> in lung, uterus, Fallopian tube and mammary gland, which correlated with detection of SCGB 1A1 protein by immunohistochemistry. Significantly altered expression of the ratio of <jats:italic>SCGB1A1A<\/jats:italic> to <jats:italic>SCGB1A1<\/jats:italic> was detected in RAO-affected animals compared to controls, suggesting different roles for SCGB 1A1 and SCGB 1A1A in this inflammatory condition.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This is the first report of three <jats:italic>SCGB1A1<\/jats:italic> genes in a mammal. The two expressed genes code for proteins predicted to differ in function. Alterations in the gene expression ratio in RAO suggest cell and tissue specific regulation and functions. These findings may be important for understanding of lung and reproductive conditions.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-13-712","type":"journal-article","created":{"date-parts":[[2012,12,20]],"date-time":"2012-12-20T02:45:04Z","timestamp":1355971504000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Multiple secretoglobin 1A1 genes are differentially expressed in horses"],"prefix":"10.1186","volume":"13","author":[{"given":"Olivier","family":"C\u00f4t\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon N","family":"Lillie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael Anthony","family":"Hayes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Ellen","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"van den Bosch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Katavolos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Viel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothee","family":"Bienzle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,12,19]]},"reference":[{"issue":"4","key":"4644_CR1","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1046\/j.1440-1843.2003.00493.x","volume":"8","author":"DA Knight","year":"2003","unstructured":"Knight DA, Holgate ST: The airway epithelium: structural and functional properties in health and disease. Respirology. 2003, 8 (4): 432-446. 10.1046\/j.1440-1843.2003.00493.x.","journal-title":"Respirology"},{"issue":"5 Pt 3","key":"4644_CR2","doi-asserted-by":"publisher","first-page":"S141","DOI":"10.1164\/ajrccm.158.supplement_2.13tac100a","volume":"158","author":"D Spina","year":"1998","unstructured":"Spina D: Epithelium smooth muscle regulation and interactions. Am J Respir Crit Care Med. 1998, 158 (5 Pt 3): S141-S145.","journal-title":"Am J Respir Crit Care Med"},{"key":"4644_CR3","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1111\/j.1749-6632.2000.tb05549.x","volume":"923","author":"J Klug","year":"2000","unstructured":"Klug J, Beier HM, Bernard A, Chilton BS, Fleming TP, Lehrer RI, Miele L, Pattabiraman N, Singh G: Uteroglobin\/Clara cell 10-kDa family of proteins: nomenclature committee report. Ann N Y Acad Sci. 2000, 923: 348-354.","journal-title":"Ann N Y Acad Sci"},{"key":"4644_CR4","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1111\/j.1749-6632.2000.tb05518.x","volume":"923","author":"G Singh","year":"2000","unstructured":"Singh G, Katyal SL: Clara cell proteins. Ann N Y Acad Sci. 2000, 923: 43-58.","journal-title":"Ann N Y Acad Sci"},{"issue":"6","key":"4644_CR5","doi-asserted-by":"publisher","first-page":"676","DOI":"10.3109\/14653240903313974","volume":"11","author":"AP Wong","year":"2009","unstructured":"Wong AP, Keating A, Waddell TK: Airway regeneration: the role of the Clara cell secretory protein and the cells that express it. Cytotherapy. 2009, 11 (6): 676-687. 10.3109\/14653240903313974.","journal-title":"Cytotherapy"},{"issue":"4","key":"4644_CR6","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1354\/vp.08-VP-0255-B-FL","volume":"46","author":"P Katavolos","year":"2009","unstructured":"Katavolos P, Ackerley CA, Viel L, Clark ME, Wen X, Bienzle D: Clara cell secretory protein is reduced in equine recurrent airway obstruction. Vet Pathol. 2009, 46 (4): 604-613. 10.1354\/vp.08-VP-0255-B-FL.","journal-title":"Vet Pathol"},{"issue":"3","key":"4644_CR7","first-page":"366","volume":"127","author":"DB Gail","year":"1983","unstructured":"Gail DB, Lenfant CJ: Cells of the lung: biology and clinical implications. Am Rev Respir Dis. 1983, 127 (3): 366-387.","journal-title":"Am Rev Respir Dis"},{"issue":"2","key":"4644_CR8","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1165\/ajrcmb.27.2.200200270c","volume":"27","author":"BR Stripp","year":"2002","unstructured":"Stripp BR, Reynolds SD, Boe IM, Lund J, Power JH, Coppens JT, Wong V, Reynolds PR, Plopper CG: Clara cell secretory protein deficiency alters clara cell secretory apparatus and the protein composition of airway lining fluid. Am J Respir Cell Mol Biol. 2002, 27 (2): 170-178.","journal-title":"Am J Respir Cell Mol Biol"},{"key":"4644_CR9","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1111\/j.1749-6632.2000.tb05531.x","volume":"923","author":"BR Stripp","year":"2000","unstructured":"Stripp BR, Reynolds SD, Plopper CG, Boe IM, Lund J: Pulmonary phenotype of CCSP\/UG deficient mice: a consequence of CCSP deficiency or altered Clara cell function?. Ann N Y Acad Sci. 2000, 923: 202-209.","journal-title":"Ann N Y Acad Sci"},{"issue":"4","key":"4644_CR10","doi-asserted-by":"publisher","first-page":"476","DOI":"10.2460\/ajvr.71.4.476","volume":"71","author":"CS Venugopal","year":"2010","unstructured":"Venugopal CS, Mendes LC, Peiro JR, Laborde SS, Stokes AM, Moore RM: Transcriptional changes associated with recurrent airway obstruction in affected and unaffected horses. Am J Vet Res. 2010, 71 (4): 476-482. 10.2460\/ajvr.71.4.476.","journal-title":"Am J Vet Res"},{"issue":"6","key":"4644_CR11","doi-asserted-by":"publisher","first-page":"1723","DOI":"10.1095\/biolreprod66.6.1723","volume":"66","author":"F Muller-Schottle","year":"2002","unstructured":"Muller-Schottle F, Bogusz A, Grotzinger J, Herrler A, Krusche CA, Beier-Hellwig K, Beier HM: Full-length complementary DNA and the derived amino acid sequence of horse uteroglobin. Biol Reprod. 2002, 66 (6): 1723-1728. 10.1095\/biolreprod66.6.1723.","journal-title":"Biol Reprod"},{"issue":"16","key":"4644_CR12","doi-asserted-by":"publisher","first-page":"4853","DOI":"10.1073\/pnas.79.16.4853","volume":"79","author":"C Menne","year":"1982","unstructured":"Menne C, Suske G, Arnemann J, Wenz M, Cato AC, Beato M: Isolation and structure of the gene for the progesterone-inducible protein uteroglobin. Proc Natl Acad Sci USA. 1982, 79 (16): 4853-4857. 10.1073\/pnas.79.16.4853.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6","key":"4644_CR13","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1093\/hmg\/1.6.371","volume":"1","author":"M Wolf","year":"1992","unstructured":"Wolf M, Klug J, Hackenberg R, Gessler M, Grzeschik KH, Beato M, Suske G: Human CC10, the homologue of rabbit uteroglobin: genomic cloning, chromosomal localization and expression in endometrial cell lines. Hum Mol Genet. 1992, 1 (6): 371-378. 10.1093\/hmg\/1.6.371.","journal-title":"Hum Mol Genet"},{"issue":"3","key":"4644_CR14","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1165\/ajrcmb.15.3.8810640","volume":"15","author":"S Hashimoto","year":"1996","unstructured":"Hashimoto S, Nakagawa K, Sueishi K: Monkey Clara cell 10\u2009kDa protein (CC10): a characterization of the amino acid sequence with an evolutional comparison with humans, rabbits, rats, and mice. Am J Respir Cell Mol Biol. 1996, 15 (3): 361-366.","journal-title":"Am J Respir Cell Mol Biol"},{"issue":"1","key":"4644_CR15","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1006\/geno.1994.1123","volume":"20","author":"BR Stripp","year":"1994","unstructured":"Stripp BR, Huffman JA, Bohinski RJ: Structure and regulation of the murine Clara cell secretory protein gene. Genomics. 1994, 20 (1): 27-35. 10.1006\/geno.1994.1123.","journal-title":"Genomics"},{"issue":"8","key":"4644_CR16","doi-asserted-by":"publisher","first-page":"2257","DOI":"10.1093\/nar\/11.8.2257","volume":"11","author":"G Suske","year":"1983","unstructured":"Suske G, Wenz M, Cato AC, Beato M: The uteroglobin gene region: hormonal regulation, repetitive elements and complete nucleotide sequence of the gene. Nucleic Acids Res. 1983, 11 (8): 2257-2271. 10.1093\/nar\/11.8.2257.","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"4644_CR17","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1089\/dna.1997.16.73","volume":"16","author":"Z Zhang","year":"1997","unstructured":"Zhang Z, Zimonjic DB, Popescu NC, Wang N, Gerhard DS, Stone EM, Arbour NC, De Vries HG, Scheffer H, Gerritsen J, et al: Human uteroglobin gene: structure, subchromosomal localization, and polymorphism. DNA Cell Biol. 1997, 16 (1): 73-83. 10.1089\/dna.1997.16.73.","journal-title":"DNA Cell Biol"},{"issue":"Suppl 2","key":"4644_CR18","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1111\/j.1439-0531.2008.01167.x","volume":"43","author":"MA Hayes","year":"2008","unstructured":"Hayes MA, Quinn BA, Keirstead ND, Katavolos P, Waelchli RO, Betteridge KJ: Proteins associated with the early intrauterine equine conceptus. Reproduction in domestic animals\u2009=\u2009Zuchthygiene. 2008, 43 (Suppl 2): 232-237.","journal-title":"Reproduction in domestic animals\u2009=\u2009Zuchthygiene"},{"issue":"5954","key":"4644_CR19","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1126\/science.1178158","volume":"326","author":"CM Wade","year":"2009","unstructured":"Wade CM, Giulotto E, Sigurdsson S, Zoli M, Gnerre S, Imsland F, Lear TL, Adelson DL, Bailey E, Bellone RR, et al: Genome sequence, comparative analysis, and population genetics of the domestic horse. Science. 2009, 326 (5954): 865-867. 10.1126\/science.1178158.","journal-title":"Science"},{"issue":"6","key":"4644_CR20","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1186\/1479-7364-5-6-691","volume":"5","author":"BC Jackson","year":"2011","unstructured":"Jackson BC, Thompson DC, Wright MW, McAndrews M, Bernard A, Nebert DW, Vasiliou V: Update of the human secretoglobin (SCGB) gene superfamily and an example of \u2018evolutionary bloom\u2019 of androgen-binding protein genes within the mouse Scgb gene superfamily. Hum Genomics. 2011, 5 (6): 691-702.","journal-title":"Hum Genomics"},{"issue":"10","key":"4644_CR21","doi-asserted-by":"publisher","first-page":"e65","DOI":"10.1093\/nar\/gkr068","volume":"39","author":"A Magi","year":"2011","unstructured":"Magi A, Benelli M, Yoon S, Roviello F, Torricelli F: Detecting common copy number variants in high-throughput sequencing data by using JointSLM algorithm. Nucleic Acids Res. 2011, 39 (10): e65-10.1093\/nar\/gkr068.","journal-title":"Nucleic Acids Res"},{"issue":"5","key":"4644_CR22","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1101\/gr.128991.111","volume":"22","author":"R Doan","year":"2012","unstructured":"Doan R, Cohen N, Harrington J, Veazy K, Juras R, Cothran G, McCue ME, Skow L, Dindot SV: Identification of copy number variants in horses. Genome Res. 2012, 22 (5): 899-907. 10.1101\/gr.128991.111.","journal-title":"Genome Res"},{"key":"4644_CR23","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1186\/1471-2164-13-78","volume":"13","author":"R Doan","year":"2012","unstructured":"Doan R, Cohen ND, Sawyer J, Ghaffari N, Johnson CD, Dindot SV: Whole-Genome sequencing and genetic variant analysis of a quarter Horse mare. BMC Genomics. 2012, 13: 78-10.1186\/1471-2164-13-78.","journal-title":"BMC Genomics"},{"issue":"7","key":"4644_CR24","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1210\/er.2007-0018","volume":"28","author":"AB Mukherjee","year":"2007","unstructured":"Mukherjee AB, Zhang Z, Chilton BS: Uteroglobin: a steroid-inducible immunomodulatory protein that founded the Secretoglobin superfamily. Endocr Rev. 2007, 28 (7): 707-725. 10.1210\/er.2007-0018.","journal-title":"Endocr Rev"},{"key":"4644_CR25","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1111\/j.1749-6632.2000.tb05522.x","volume":"923","author":"I Callebaut","year":"2000","unstructured":"Callebaut I, Poupon A, Bally R, Demaret JP, Housset D, Delettre J, Hossenlopp P, Mornon JP: The uteroglobin fold. Ann N Y Acad Sci. 2000, 923: 90-112.","journal-title":"Ann N Y Acad Sci"},{"issue":"2","key":"4644_CR26","doi-asserted-by":"publisher","first-page":"155","DOI":"10.3109\/01902148009069645","volume":"1","author":"CG Plopper","year":"1980","unstructured":"Plopper CG, Mariassy AT, Hill LH: Ultrastructure of the nonciliated bronchiolar epithelial (Clara) cell of mammalian lung: II. A comparison of horse, steer, sheep, dog, and cat. Exp Lung Res. 1980, 1 (2): 155-169. 10.3109\/01902148009069645.","journal-title":"Exp Lung Res"},{"issue":"16","key":"4644_CR27","doi-asserted-by":"publisher","first-page":"13295","DOI":"10.1074\/jbc.M010679200","volume":"276","author":"R Burmeister","year":"2001","unstructured":"Burmeister R, Boe IM, Nykjaer A, Jacobsen C, Moestrup SK, Verroust P, Christensen EI, Lund J, Willnow TE: A two-receptor pathway for catabolism of Clara cell secretory protein in the kidney. J Biol Chem. 2001, 276 (16): 13295-13301. 10.1074\/jbc.M010679200.","journal-title":"J Biol Chem"},{"key":"4644_CR28","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.gene.2005.10.027","volume":"369","author":"Z Zhang","year":"2006","unstructured":"Zhang Z, Kim SJ, Chowdhury B, Wang J, Lee YC, Tsai PC, Choi M, Mukherjee AB: Interaction of uteroglobin with lipocalin-1 receptor suppresses cancer cell motility and invasion. Gene. 2006, 369: 66-71.","journal-title":"Gene"},{"issue":"23","key":"4644_CR29","doi-asserted-by":"publisher","first-page":"20206","DOI":"10.1074\/jbc.M101762200","volume":"276","author":"P Wojnar","year":"2001","unstructured":"Wojnar P, Lechner M, Merschak P, Redl B: Molecular cloning of a novel lipocalin-1 interacting human cell membrane receptor using phage display. J Biol Chem. 2001, 276 (23): 20206-20212. 10.1074\/jbc.M101762200.","journal-title":"J Biol Chem"},{"key":"4644_CR30","first-page":"319","volume":"8","author":"O Janne","year":"1980","unstructured":"Janne O, Hemminki S, Isomaa VV, Isotalo H, Kopu H, Orava M, Torkkeli T: Uteroglobin synthesis and its relationship to changes in progesterone receptors, RNA polymerase and poly-A polymerases in the rabbit uterus. Dev Endocrinol. 1980, 8: 319-340.","journal-title":"Dev Endocrinol"},{"issue":"5 Pt 1","key":"4644_CR31","doi-asserted-by":"publisher","first-page":"1585","DOI":"10.1164\/ajrccm.159.5.9806044","volume":"159","author":"JE Boers","year":"1999","unstructured":"Boers JE, Ambergen AW, Thunnissen FB: Number and proliferation of clara cells in normal human airway epithelium. Am J Respir Crit Care Med. 1999, 159 (5 Pt 1): 1585-1591.","journal-title":"Am J Respir Crit Care Med"},{"issue":"5","key":"4644_CR32","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1089\/dna.1994.13.495","volume":"13","author":"A Peri","year":"1994","unstructured":"Peri A, Cowan BD, Bhartiya D, Miele L, Nieman LK, Nwaeze IO, Mukherjee AB: Expression of Clara cell 10-kD gene in the human endometrium and its relationship to ovarian menstrual cycle. DNA Cell Biol. 1994, 13 (5): 495-503. 10.1089\/dna.1994.13.495.","journal-title":"DNA Cell Biol"},{"issue":"6","key":"4644_CR33","doi-asserted-by":"publisher","first-page":"1613","DOI":"10.1016\/j.fertnstert.2007.03.071","volume":"89","author":"AA Quintar","year":"2008","unstructured":"Quintar AA, Mukdsi JH, del Valle Bonaterra M, Aoki A, Maldonado CA, Perez Alzaa J: Increased expression of uteroglobin associated with tubal inflammation and ectopic pregnancy. Fertil Steril. 2008, 89 (6): 1613-1617. 10.1016\/j.fertnstert.2007.03.071.","journal-title":"Fertil Steril"},{"issue":"1","key":"4644_CR34","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/S0022-5347(17)41522-9","volume":"140","author":"MJ Manyak","year":"1988","unstructured":"Manyak MJ, Kikukawa T, Mukherjee AB: Expression of a uteroglobin-like protein in human prostate. J Urol. 1988, 140 (1): 176-182.","journal-title":"J Urol"},{"issue":"12 Pt 1","key":"4644_CR35","first-page":"2295","volume":"3","author":"AT Weeraratna","year":"1997","unstructured":"Weeraratna AT, Cajigas JA, Schwartz A, Enquist EG, Manyak MJ, Patierno SR: Loss of uteroglobin expression in prostate cancer: relationship to advancing grade. Clin Cancer Res. 1997, 3 (12 Pt 1): 2295-2300.","journal-title":"Clin Cancer Res"},{"issue":"5","key":"4644_CR36","doi-asserted-by":"publisher","first-page":"2099","DOI":"10.1172\/JCI116810","volume":"92","author":"A Peri","year":"1993","unstructured":"Peri A, Cordella-Miele E, Miele L, Mukherjee AB: Tissue-specific expression of the gene coding for human Clara cell 10-kD protein, a phospholipase A2-inhibitory protein. J Clin Invest. 1993, 92 (5): 2099-2109. 10.1172\/JCI116810.","journal-title":"J Clin Invest"},{"key":"4644_CR37","first-page":"135","volume":"231","author":"AB Mukherjee","year":"1988","unstructured":"Mukherjee AB, Cordella-Miele E, Kikukawa T, Miele L: Modulation of cellular response to antigens by uteroglobin and transglutaminase. Adv Exp Med Biol. 1988, 231: 135-152.","journal-title":"Adv Exp Med Biol"},{"issue":"2","key":"4644_CR38","doi-asserted-by":"publisher","first-page":"118","DOI":"10.3816\/CGC.2002.n.014","volume":"1","author":"SR Patierno","year":"2002","unstructured":"Patierno SR, Manyak MJ, Fernandez PM, Baker A, Weeraratna AT, Chou DS, Szlyk G, Geib KS, Walsh C, Patteras J: Uteroglobin: a potential novel tumor suppressor and molecular therapeutic for prostate cancer. Clin Prostate Cancer. 2002, 1 (2): 118-124.","journal-title":"Clin Prostate Cancer"},{"issue":"5","key":"4644_CR39","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1016\/j.cell.2011.02.013","volume":"144","author":"D Hanahan","year":"2011","unstructured":"Hanahan D, Weinberg RA: Hallmarks of cancer: the next generation. Cell. 2011, 144 (5): 646-674. 10.1016\/j.cell.2011.02.013.","journal-title":"Cell"},{"issue":"3","key":"4644_CR40","doi-asserted-by":"publisher","first-page":"626","DOI":"10.1111\/j.1939-1676.2009.0292.x","volume":"23","author":"V Gerber","year":"2009","unstructured":"Gerber V, Baleri D, Klukowska-Rotzler J, Swinburne JE, Dolf G: Mixed inheritance of equine recurrent airway obstruction. J Vet Intern Med. 2009, 23 (3): 626-630. 10.1111\/j.1939-1676.2009.0292.x.","journal-title":"J Vet Intern Med"},{"key":"4644_CR41","volume-title":"Geneious V5.5.3","author":"AB Drummond AJ","year":"2011","unstructured":"Drummond AJ AB, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A: Geneious V5.5.3. 2011"}],"container-title":["BMC Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2164-13-712.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T21:52:52Z","timestamp":1630533172000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcgenomics.biomedcentral.com\/articles\/10.1186\/1471-2164-13-712"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["4644"],"URL":"https:\/\/doi.org\/10.1186\/1471-2164-13-712","relation":{},"ISSN":["1471-2164"],"issn-type":[{"value":"1471-2164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]},"assertion":[{"value":"20 April 2012","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 December 2012","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 December 2012","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"712"}}