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TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      and TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      LS are suitable for simultaneous isolation of RNA, DNA and proteins from the same biological sample. These reagents are widely used for RNA and\/or DNA isolation, while reports on their use for protein extraction are limited, attributable to technical difficulties in protein solubilisation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      LS was used for RNA isolation from 284 human colon cancer samples, including normal colon mucosa, tubulovillous adenomas, and colon carcinomas with proficient and deficient mismatch repair system. TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      was used for RNA isolation from human colon cancer cells, from brains of transgenic Alzheimer\u2019s disease mice model, and from cultured mouse cortical neurons. Following RNA extraction, the TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      -chloroform fractions from human colon cancer samples and from mouse hippocampus and frontal cortex were stored for 2 years and 3 months, respectively, at \u221280\u00b0C until used for protein isolation.\n                    <\/jats:p>\n                    <jats:p>\n                      Simple modifications to the TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      manufacturer\u2019s protocol, including Urea:SDS solubilization and sonication, allowed improved protein recovery yield compared to the TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      manufacturer\u2019s protocol. Following SDS-PAGE and Ponceau and Coomassie staining, recovered proteins displayed wide molecular weight range and staining pattern comparable to those obtainable with commonly used protein extraction protocols. We also show that nuclear and cytosolic proteins can be easily extracted and detected by immunoblotting, and that posttranslational modifications, such as protein phosphorylation, are detectable in proteins recovered from TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      -chloroform fractions stored for up to 2 years at \u221280\u00b0C.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      We provide a novel approach to improve protein recovery from samples processed for nucleic acid extraction with TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      and TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      LS compared to the manufacturer`s protocol, allowing downstream immunoblotting and evaluation of steady-state relative protein expression levels. The method was validated in large sets of samples from multiple sources, including human colon cancer and brains of transgenic Alzheimer\u2019s disease mice model, stored in TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      -chloroform for up to two years. Collectively, we provide a faster and cheaper alternative to the TRIzol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      manufacturer`s protein extraction protocol, illustrating the high relevance, and wide applicability, of the present protein isolation method for the immunoblot evaluation of steady-state relative protein expression levels in samples from multiple sources, and following prolonged storage.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/1471-2164-14-181","type":"journal-article","created":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T15:45:01Z","timestamp":1363362301000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":114,"title":["Efficient recovery of proteins from multiple source samples after trizol\u00ae or trizol\u00aeLS RNA extraction and long-term storage"],"prefix":"10.1186","volume":"14","author":[{"given":"Andr\u00e9 ES","family":"Sim\u00f5es","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane M","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana D","family":"Amaral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana F","family":"Nunes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia E","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro M","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian C","family":"Lo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudi","family":"D'Hooge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clifford J","family":"Steer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen N","family":"Thibodeau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro M","family":"Borralho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cec\u00edlia MP","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,3,15]]},"reference":[{"issue":"3","key":"4893_CR1","first-page":"532","volume":"15","author":"P Chomczynski","year":"1993","unstructured":"Chomczynski P: A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. 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