{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:15:24Z","timestamp":1790248524021,"version":"4.1.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Genomics"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Shigella flexneri<\/jats:italic> is the major cause of shigellosis in the developing countries. The O-antigen component of the lipopolysaccharide is one of the key virulence determinants required for the pathogenesis of <jats:italic>S. flexneri<\/jats:italic>. The glucosyltransferase and\/or acetyltransferase genes responsible for the modification of the O-antigen are encoded by temperate serotype converting bacteriophage present in the <jats:italic>S. flexneri<\/jats:italic> genome. Several serotype converting phages have previously been isolated and characterized, however, attempts to isolate a serotype converting phage which encodes the modification genes of serotypes 4a strain have not been successful.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In this study, a novel temperate serotype converting bacteriophage SfIV was isolated. Lysogenisation of phage SfIV converted serotype Y strain to serotype 4a. Electron microscopy indicated that SfIV belongs to <jats:italic>Myoviridae<\/jats:italic> family. The 39,758\u00a0bp genome of phage SfIV encompasses 54 open reading frames (<jats:italic>orfs<\/jats:italic>). Protein level comparison of SfIV with other serotype converting phages of <jats:italic>S. flexneri<\/jats:italic> revealed that SfIV is similar to phage SfII and SfV. The comparative analysis also revealed that SfIV phage contained five proteins which were not found in any other phages of <jats:italic>S. flexneri.<\/jats:italic> These proteins were: a tail fiber assembly protein, two hypothetical proteins with no clear function, and two other unknown proteins which were encoded by <jats:italic>orfs<\/jats:italic> present on a moron, that presumably got introduced in SfIV genome from another species via a transposon. These unique proteins of SfIV may play a role in the pathogenesis of the host.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This study reports the isolation and complete genome sequence analysis of bacteriophage SfIV. The SfIV phage has a host range significantly different from the other phages of <jats:italic>Shigella<\/jats:italic>. Comparative genome analysis identified several proteins unique to SfIV, which may potentially be involved in the survival and pathogenesis of its host. These findings will further our understanding on the evolution of these phages, and will also facilitate studies on development of new phage vectors and therapeutic agents to control infections caused by <jats:italic>S. flexneri.<\/jats:italic>\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-14-677","type":"journal-article","created":{"date-parts":[[2013,10,3]],"date-time":"2013-10-03T13:07:50Z","timestamp":1380805670000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Isolation, characterization and comparative genomics of bacteriophage SfIV: a novel serotype converting phage from Shigella flexneri"],"prefix":"10.1186","volume":"14","author":[{"given":"Richa","family":"Jakhetia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaisar A","family":"Talukder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naresh K","family":"Verma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,10,3]]},"reference":[{"issue":"8","key":"5383_CR1","first-page":"651","volume":"77","author":"KL Kotloff","year":"1999","unstructured":"Kotloff KL, Winickoff JP, Ivanoff B, Clemens JD, Swerdlow DL, Sansonetti PJ, Adak GK, Levine MM: Global burden of Shigella infections: implications for vaccine development and implementation of control strategies. Bull World Health Organ. 1999, 77 (8): 651-666.","journal-title":"Bull World Health Organ"},{"issue":"1","key":"5383_CR2","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1128\/CMR.00032-07","volume":"21","author":"GN Schroeder","year":"2008","unstructured":"Schroeder GN, Hilbi H: Molecular pathogenesis of Shigella spp.: controlling host cell signaling, invasion, and death by type III secretion. Clin Microbiol Rev. 2008, 21 (1): 134-156. 10.1128\/CMR.00032-07.","journal-title":"Clin Microbiol Rev"},{"issue":"2","key":"5383_CR3","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1128\/JCM.00614-09","volume":"48","author":"C Ye","year":"2010","unstructured":"Ye C, Lan R, Xia S, Zhang J, Sun Q, Zhang S, Jing H, Wang L, Li Z, Zhou Z: Emergence of a new multidrug-resistant serotype X variant in an epidemic clone of Shigella flexneri. J Clin Microbiol. 2010, 48 (2): 419-426. 10.1128\/JCM.00614-09.","journal-title":"J Clin Microbiol"},{"issue":"4","key":"5383_CR4","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1093\/glycob\/cws222","volume":"23","author":"YA Knirel","year":"2013","unstructured":"Knirel YA, Lan R, Senchenkova SN, Wang J, Shashkov AS, Wang Y, Perepelov AV, Xiong Y, Xu J, Sun Q: O-antigen structure of Shigella flexneri serotype Yv and effect of the lpt-O gene variation on phosphoethanolamine modification of S. flexneri O-antigens. Glycobiology. 2013, 23 (4): 475-485. 10.1093\/glycob\/cws222.","journal-title":"Glycobiology"},{"issue":"3","key":"5383_CR5","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/j.diagmicrobio.2012.06.022","volume":"74","author":"X Luo","year":"2012","unstructured":"Luo X, Sun Q, Lan R, Wang J, Li Z, Xia S, Zhang J, Wang Y, Jin D, Wang Y: Emergence of a novel Shigella flexneri serotype 1d in China. Diagn Microbiol Infect Dis. 2012, 74 (3): 316-319. 10.1016\/j.diagmicrobio.2012.06.022.","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"4","key":"5383_CR6","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1099\/00222615-23-4-289","volume":"23","author":"DA Simmons","year":"1987","unstructured":"Simmons DA, Romanowska E: Structure and biology of Shigella flexneri O antigens. J Med Microbiol. 1987, 23 (4): 289-302. 10.1099\/00222615-23-4-289.","journal-title":"J Med Microbiol"},{"issue":"1","key":"5383_CR7","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/S0966-842X(99)01646-7","volume":"8","author":"GE Allison","year":"2000","unstructured":"Allison GE, Verma NK: Serotype-converting bacteriophages and O-antigen modification in Shigella flexneri. Trends Microbiol. 2000, 8 (1): 17-23. 10.1016\/S0966-842X(99)01646-7.","journal-title":"Trends Microbiol"},{"issue":"Pt 4","key":"5383_CR8","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1099\/00221287-147-4-851","volume":"147","author":"MM Adams","year":"2001","unstructured":"Adams MM, Allison GE, Verma NK: Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296. Microbiology. 2001, 147 (Pt 4): 851-860.","journal-title":"Microbiology"},{"issue":"2","key":"5383_CR9","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/S0378-1119(97)00143-1","volume":"195","author":"PT Huan","year":"1997","unstructured":"Huan PT, Bastin DA, Whittle BL, Lindberg AA, Verma NK: Molecular characterization of the genes involved in O-antigen modification, attachment, integration and excision in Shigella flexneri bacteriophage SfV. Gene. 1997, 195 (2): 217-227. 10.1016\/S0378-1119(97)00143-1.","journal-title":"Gene"},{"issue":"2","key":"5383_CR10","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/S0378-1119(97)00144-3","volume":"195","author":"PT Huan","year":"1997","unstructured":"Huan PT, Whittle BL, Bastin DA, Lindberg AA, Verma NK: Shigella flexneri type-specific antigen V: cloning, sequencing and characterization of the glucosyl transferase gene of temperate bacteriophage SfV. Gene. 1997, 195 (2): 207-216. 10.1016\/S0378-1119(97)00144-3.","journal-title":"Gene"},{"issue":"4","key":"5383_CR11","doi-asserted-by":"publisher","first-page":"1252","DOI":"10.1016\/j.bbrc.2005.01.087","volume":"328","author":"H Korres","year":"2005","unstructured":"Korres H, Mavris M, Morona R, Manning PA, Verma NK: Topological analysis of GtrA and GtrB proteins encoded by the serotype-converting cassette of Shigella flexneri. Biochem Biophys Res Commun. 2005, 328 (4): 1252-1260. 10.1016\/j.bbrc.2005.01.087.","journal-title":"Biochem Biophys Res Commun"},{"issue":"5","key":"5383_CR12","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1046\/j.1365-2958.1997.6301997.x","volume":"26","author":"M Mavris","year":"1997","unstructured":"Mavris M, Manning PA, Morona R: Mechanism of bacteriophage SfII-mediated serotype conversion in Shigella flexneri. Mol Microbiol. 1997, 26 (5): 939-950. 10.1046\/j.1365-2958.1997.6301997.x.","journal-title":"Mol Microbiol"},{"issue":"21","key":"5383_CR13","doi-asserted-by":"publisher","first-page":"6612","DOI":"10.1128\/JB.00628-09","volume":"191","author":"RM Stagg","year":"2009","unstructured":"Stagg RM, Tang SS, Carlin NI, Talukder KA, Cam PD, Verma NK: A novel glucosyltransferase involved in O-antigen modification of Shigella flexneri serotype 1c. J Bacteriol. 2009, 191 (21): 6612-6617. 10.1128\/JB.00628-09.","journal-title":"J Bacteriol"},{"issue":"1","key":"5383_CR14","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/0378-1119(93)90702-5","volume":"129","author":"NK Verma","year":"1993","unstructured":"Verma NK, Verma DJ, Huan PT, Lindberg AA: Cloning and sequencing of the glucosyl transferase-encoding gene from converting bacteriophage X (SFX) of Shigella flexneri. Gene. 1993, 129 (1): 99-101. 10.1016\/0378-1119(93)90702-5.","journal-title":"Gene"},{"issue":"2","key":"5383_CR15","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1016\/j.jmb.2004.03.068","volume":"339","author":"S Casjens","year":"2004","unstructured":"Casjens S, Winn-Stapley DA, Gilcrease EB, Morona R, Kuhlewein C, Chua JE, Manning PA, Inwood W, Clark AJ: The chromosome of Shigella flexneri bacteriophage Sf6: complete nucleotide sequence, genetic mosaicism, and DNA packaging. J Mol Biol. 2004, 339 (2): 379-394. 10.1016\/j.jmb.2004.03.068.","journal-title":"J Mol Biol"},{"issue":"7","key":"5383_CR16","doi-asserted-by":"publisher","first-page":"1974","DOI":"10.1128\/JB.184.7.1974-1987.2002","volume":"184","author":"GE Allison","year":"2002","unstructured":"Allison GE, Angeles D, Tran-Dinh N, Verma NK: Complete genomic sequence of SfV, a serotype-converting temperate bacteriophage of Shigella flexneri. J Bacteriol. 2002, 184 (7): 1974-1987. 10.1128\/JB.184.7.1974-1987.2002.","journal-title":"J Bacteriol"},{"issue":"1","key":"5383_CR17","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/S0042-6822(03)00198-3","volume":"308","author":"GE Allison","year":"2003","unstructured":"Allison GE, Angeles DC, Huan P, Verma NK: Morphology of temperate bacteriophage SfV and characterisation of the DNA packaging and capsid genes: the structural genes evolved from two different phage families. Virology. 2003, 308 (1): 114-127. 10.1016\/S0042-6822(03)00198-3.","journal-title":"Virology"},{"issue":"Pt 5","key":"5383_CR18","doi-asserted-by":"publisher","first-page":"1263","DOI":"10.1099\/13500872-145-5-1263","volume":"145","author":"S Guan","year":"1999","unstructured":"Guan S, Bastin DA, Verma NK: Functional analysis of the O antigen glucosylation gene cluster of Shigella flexneri bacteriophage SfX. Microbiology. 1999, 145 (Pt 5): 1263-1273.","journal-title":"Microbiology"},{"issue":"1","key":"5383_CR19","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1186\/1471-2180-13-39","volume":"13","author":"Q Sun","year":"2013","unstructured":"Sun Q, Lan R, Wang Y, Wang J, Wang Y, Li P, Du P, Xu J: Isolation and genomic characterization of SfI, a serotype-converting bacteriophage of Shigella flexneri. BMC microbiology. 2013, 13 (1): 39-10.1186\/1471-2180-13-39.","journal-title":"BMC microbiology"},{"issue":"2","key":"5383_CR20","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/S0264-410X(88)80018-5","volume":"6","author":"AA Lindberg","year":"1988","unstructured":"Lindberg AA, Karnell A, Stocker BA, Katakura S, Sweiha H, Reinholt FP: Development of an auxotrophic oral live Shigella flexneri vaccine. Vaccine. 1988, 6 (2): 146-150. 10.1016\/S0264-410X(88)80018-5.","journal-title":"Vaccine"},{"key":"5383_CR21","volume-title":"Molecular Cloning: a Laboratory Manual","author":"J Sambrook","year":"1989","unstructured":"Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: a Laboratory Manual. 1989, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 2,","edition":"2"},{"issue":"Web Server issu","key":"5383_CR22","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1093\/nar\/gki366","volume":"33","author":"P Schattner","year":"2005","unstructured":"Schattner P, Brooks AN, Lowe TM: The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs. Nucleic acids research. 2005, 33 (Web Server issue): 686-689.","journal-title":"Nucleic acids research"},{"issue":"5","key":"5383_CR23","doi-asserted-by":"publisher","first-page":"1003","DOI":"10.1006\/jmbi.2001.5102","volume":"313","author":"D Gautheret","year":"2001","unstructured":"Gautheret D, Lambert A: Direct RNA motif definition and identification from multiple sequence alignments using secondary structure profiles. J Mol Biol. 2001, 313 (5): 1003-1011. 10.1006\/jmbi.2001.5102.","journal-title":"J Mol Biol"},{"issue":"6","key":"5383_CR24","doi-asserted-by":"publisher","first-page":"e11147","DOI":"10.1371\/journal.pone.0011147","volume":"5","author":"AE Darling","year":"2010","unstructured":"Darling AE, Mau B, Perna NT: progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PloS one. 2010, 5 (6): e11147-10.1371\/journal.pone.0011147.","journal-title":"PloS one"},{"issue":"22","key":"5383_CR25","doi-asserted-by":"publisher","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","volume":"22","author":"JD Thompson","year":"1994","unstructured":"Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22 (22): 4673-4680. 10.1093\/nar\/22.22.4673.","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"5383_CR26","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1128\/br.31.4.230-314.1967","volume":"31","author":"DE Bradley","year":"1967","unstructured":"Bradley DE: Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967, 31 (4): 230-314.","journal-title":"Bacteriol Rev"},{"key":"5383_CR27","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1128\/genomeA.00626-13","volume":"1","author":"DT George","year":"2013","unstructured":"George DT, Stephenson DP, Tran E, Morona R, Verma NK: Complete Genome Sequence of SfII, a Serotype-Converting Bacteriophage of the Highly Prevalent Shigella flexneri Serotype 2a. Genome announcements. 2013, 1: 5-","journal-title":"Genome announcements"},{"issue":"5","key":"5383_CR28","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1101\/gr.074492.107","volume":"18","author":"DR Zerbino","year":"2008","unstructured":"Zerbino DR, Birney E: Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 2008, 18 (5): 821-829. 10.1101\/gr.074492.107.","journal-title":"Genome Res"},{"key":"5383_CR29","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1146\/annurev.genet.42.110807.091545","volume":"42","author":"VB Rao","year":"2008","unstructured":"Rao VB, Feiss M: The bacteriophage DNA packaging motor. Annu Rev Genet. 2008, 42: 647-681. 10.1146\/annurev.genet.42.110807.091545.","journal-title":"Annu Rev Genet"},{"issue":"9","key":"5383_CR30","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1046\/j.1365-2443.1997.1450343.x","volume":"2","author":"H Fujisawa","year":"1997","unstructured":"Fujisawa H, Morita M: Phage DNA packaging. Genes Cells. 1997, 2 (9): 537-545.","journal-title":"Genes Cells"},{"issue":"9","key":"5383_CR31","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1038\/nrmicro2632","volume":"9","author":"SR Casjens","year":"2011","unstructured":"Casjens SR: The DNA-packaging nanomotor of tailed bacteriophages. Nat Rev Microbiol. 2011, 9 (9): 647-657. 10.1038\/nrmicro2632.","journal-title":"Nat Rev Microbiol"},{"issue":"2","key":"5383_CR32","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.virol.2012.01.040","volume":"427","author":"KN Parent","year":"2012","unstructured":"Parent KN, Gilcrease EB, Casjens SR, Baker TS: Structural evolution of the P22-like phages: comparison of Sf6 and P22 procapsid and virion architectures. Virology. 2012, 427 (2): 177-188. 10.1016\/j.virol.2012.01.040.","journal-title":"Virology"},{"issue":"5","key":"5383_CR33","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1006\/jmbi.1996.0281","volume":"258","author":"F Tetart","year":"1996","unstructured":"Tetart F, Repoila F, Monod C, Krisch HM: Bacteriophage T4 host range is expanded by duplications of a small domain of the tail fiber adhesin. J Mol Biol. 1996, 258 (5): 726-731. 10.1006\/jmbi.1996.0281.","journal-title":"J Mol Biol"},{"issue":"3","key":"5383_CR34","doi-asserted-by":"publisher","first-page":"1542","DOI":"10.1074\/jbc.M205294200","volume":"278","author":"A Freiberg","year":"2003","unstructured":"Freiberg A, Morona R, Van den Bosch L, Jung C, Behlke J, Carlin N, Seckler R, Baxa U: The tailspike protein of Shigella phage Sf6. A structural homolog of Salmonella phage P22 tailspike protein without sequence similarity in the beta-helix domain. J Biol Chem. 2003, 278 (3): 1542-1548. 10.1074\/jbc.M205294200.","journal-title":"J Biol Chem"},{"issue":"1","key":"5383_CR35","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/S0092-8674(85)80121-5","volume":"42","author":"EJ Grayhack","year":"1985","unstructured":"Grayhack EJ, Yang XJ, Lau LF, Roberts JW: Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site. Cell. 1985, 42 (1): 259-269. 10.1016\/S0092-8674(85)80121-5.","journal-title":"Cell"},{"issue":"4","key":"5383_CR36","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1046\/j.1365-2958.1996.331395.x","volume":"21","author":"U Blasi","year":"1996","unstructured":"Blasi U, Young R: Two beginnings for a single purpose: the dual-start holins in the regulation of phage lysis. Mol Microbiol. 1996, 21 (4): 675-682. 10.1046\/j.1365-2958.1996.331395.x.","journal-title":"Mol Microbiol"},{"issue":"17","key":"5383_CR37","doi-asserted-by":"publisher","first-page":"9845","DOI":"10.1073\/pnas.96.17.9845","volume":"96","author":"S Mirold","year":"1999","unstructured":"Mirold S, Rabsch W, Rohde M, Stender S, Tschape H, Russmann H, Igwe E, Hardt WD: Isolation of a temperate bacteriophage encoding the type III effector protein SopE from an epidemic Salmonella typhimurium strain. Proc Natl Acad Sci USA. 1999, 96 (17): 9845-9850. 10.1073\/pnas.96.17.9845.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"1","key":"5383_CR38","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/S0378-1097(97)00415-1","volume":"156","author":"S Vica Pacheco","year":"1997","unstructured":"Vica Pacheco S, Garcia Gonzalez O, Paniagua Contreras GL: The lom gene of bacteriophage lambda is involved in Escherichia coli K12 adhesion to human buccal epithelial cells. FEMS Microbiol Lett. 1997, 156 (1): 129-132. 10.1016\/S0378-1097(97)00415-1.","journal-title":"FEMS Microbiol Lett"},{"issue":"8","key":"5383_CR39","doi-asserted-by":"publisher","first-page":"3985","DOI":"10.1128\/IAI.70.8.3985-3993.2002","volume":"70","author":"PL Wagner","year":"2002","unstructured":"Wagner PL, Waldor MK: Bacteriophage control of bacterial virulence. Infect Immun. 2002, 70 (8): 3985-3993. 10.1128\/IAI.70.8.3985-3993.2002.","journal-title":"Infect Immun"}],"container-title":["BMC Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2164-14-677.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T01:55:17Z","timestamp":1630547717000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcgenomics.biomedcentral.com\/articles\/10.1186\/1471-2164-14-677"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,3]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["5383"],"URL":"https:\/\/doi.org\/10.1186\/1471-2164-14-677","relation":{},"ISSN":["1471-2164"],"issn-type":[{"value":"1471-2164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,3]]},"assertion":[{"value":"20 March 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 September 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 October 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"677"}}