{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:15:29Z","timestamp":1760897729044},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2006,9,5]],"date-time":"2006-09-05T00:00:00Z","timestamp":1157414400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Genomics"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>More than 12,000 simple sequence repeats (SSRs) have been identified in the genome of <jats:italic>Burkholderia mallei<\/jats:italic> ATCC 23344. As a demonstrated mechanism of phase variation in other pathogenic bacteria, these may function as mutable loci leading to altered protein expression or structure variation. To determine if such alterations are occurring in vivo, the genomes of various single-colony passaged <jats:italic>B. mallei<\/jats:italic> ATCC 23344 isolates, one from each source, were sequenced from culture, a mouse, a horse, and two isolates from a single human patient, and the sequence compared to the published <jats:italic>B. mallei<\/jats:italic> ATCC 23344 genome sequence.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Forty-nine insertions and deletions (indels) were detected at SSRs in the five passaged strains, a majority of which (67.3%) were located within noncoding areas, suggesting that such regions are more tolerant of sequence alterations. Expression profiling of the two human passaged isolates compared to the strain before passage revealed alterations in the mRNA levels of multiple genes when grown in culture.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>These data support the notion that genome variability upon passage is a feature of <jats:italic>B. mallei<\/jats:italic> ATCC23344, and that within a host <jats:italic>B. mallei<\/jats:italic> generates a diverse population of clones that accumulate genome sequence variation at SSR and other loci.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-7-228","type":"journal-article","created":{"date-parts":[[2006,9,5]],"date-time":"2006-09-05T18:32:02Z","timestamp":1157481122000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Genome sequence alterations detected upon passage of Burkholderia mallei ATCC 23344 in culture and in mammalian hosts"],"prefix":"10.1186","volume":"7","author":[{"given":"Claudia M","family":"Romero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"DeShazer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara","family":"Feldblyum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Ravel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald","family":"Woods","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H Stanley","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine M","family":"Ronning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C","family":"Nierman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2006,9,5]]},"reference":[{"issue":"2","key":"611_CR1","first-page":"57","volume":"99","author":"M Alibasoglu","year":"1986","unstructured":"Alibasoglu M, Yesildere T, Calislar T, Inal T, Calsikan U: [Glanders outbreak in lions in the Istanbul zoological garden]. Berl Munch Tierarztl Wochenschr. 1986, 99 (2): 57-63.","journal-title":"Berl Munch Tierarztl Wochenschr"},{"key":"611_CR2","first-page":"268","volume":"35","author":"CD McGilvray","year":"1944","unstructured":"McGilvray CD: The transmission of glanders from horse to man. Can J Public Health. 1944, 35: 268-275.","journal-title":"Can J Public Health"},{"key":"611_CR3","first-page":"258","volume":"70","author":"H Neubauer","year":"1997","unstructured":"Neubauer H, Meyer H, Finke EJ: Human glanders. Rev Int Services Sante Forces Armees. 1997, 70: 258-265.","journal-title":"Rev Int Services Sante Forces Armees"},{"key":"611_CR4","first-page":"209","volume-title":"Infectious Diseases: Biological Weapons Defense: Infectious Diseases and Counterterrorism","author":"DM Waag","year":"2004","unstructured":"Waag DM, DeShazer D: Glanders: New insights into an old disease. Infectious Diseases: Biological Weapons Defense: Infectious Diseases and Counterterrorism. Edited by: Lindler LE, Lebeda FJ, Korch GW, and Totowa NJ. 2004, Humana Press, Inc., 209-238."},{"issue":"4","key":"611_CR5","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1056\/NEJM200107263450404","volume":"345","author":"A Srinivasan","year":"2001","unstructured":"Srinivasan A, Kraus CN, DeShazer D, Becker PM, Dick JD, Spacek L, Bartlett JG, Byrne WR, Thomas DL: Glanders in a military research microbiologist. N Engl J Med. 2001, 345 (4): 256-258. 10.1056\/NEJM200107263450404.","journal-title":"N Engl J Med"},{"issue":"16","key":"611_CR6","doi-asserted-by":"publisher","first-page":"1986","DOI":"10.1016\/j.vaccine.2004.10.017","volume":"23","author":"RL Ulrich","year":"2005","unstructured":"Ulrich RL, Amemiya K, Waag DM, Roy CJ, DeShazer D: Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice. Vaccine. 2005, 23 (16): 1986-1992. 10.1016\/j.vaccine.2004.10.017.","journal-title":"Vaccine"},{"issue":"39","key":"611_CR7","doi-asserted-by":"publisher","first-page":"14246","DOI":"10.1073\/pnas.0403306101","volume":"101","author":"WC Nierman","year":"2004","unstructured":"Nierman WC, DeShazer D, Kim HS, Tettelin H, Nelson KE, Feldblyum T, Ulrich RL, Ronning CM, Brinkac LM, Daugherty SC, Davidsen TD, Deboy RT, Dimitrov G, Dodson RJ, Durkin AS, Gwinn ML, Haft DH, Khouri H, Kolonay JF, Madupu R, Mohammoud Y, Nelson WC, Radune D, Romero CM, Sarria S, Selengut J, Shamblin C, Sullivan SA, White O, Yu Y, Zafar N, Zhou L, Fraser CM: Structural flexibility in the Burkholderia mallei genome. Proc Natl Acad Sci U S A. 2004, 101 (39): 14246-14251. 10.1073\/pnas.0403306101.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"16","key":"611_CR8","doi-asserted-by":"publisher","first-page":"6463","DOI":"10.1093\/nar\/17.16.6463","volume":"17","author":"D Tautz","year":"1989","unstructured":"Tautz D: Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Res. 1989, 17 (16): 6463-6471.","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"611_CR9","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/0888-7543(90)90195-Z","volume":"7","author":"JL Weber","year":"1990","unstructured":"Weber JL: Informativeness of human (dC-dA)n.(dG-dT)n polymorphisms. Genomics. 1990, 7 (4): 524-530. 10.1016\/0888-7543(90)90195-Z.","journal-title":"Genomics"},{"issue":"1","key":"611_CR10","first-page":"62","volume":"10","author":"R Gur-Arie","year":"2000","unstructured":"Gur-Arie R, Cohen CJ, Eitan Y, Shelef L, Hallerman EM, Kashi Y: Simple sequence repeats in Escherichia coli: abundance, distribution, composition, and polymorphism. Genome Res. 2000, 10 (1): 62-71.","journal-title":"Genome Res"},{"issue":"9-10","key":"611_CR11","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1007\/s000180050019","volume":"56","author":"A van Belkum","year":"1999","unstructured":"van Belkum A: Short sequence repeats in microbial pathogenesis and evolution. Cell Mol Life Sci. 1999, 56 (9-10): 729-734. 10.1007\/s000180050019.","journal-title":"Cell Mol Life Sci"},{"issue":"2","key":"611_CR12","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1128\/MMBR.62.2.275-293.1998","volume":"62","author":"A van Belkum","year":"1998","unstructured":"van Belkum A, Scherer S, van Alphen L, Verbrugh H: Short-sequence DNA repeats in prokaryotic genomes. Microbiol Mol Biol Rev. 1998, 62 (2): 275-293.","journal-title":"Microbiol Mol Biol Rev"},{"issue":"9-10","key":"611_CR13","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1016\/S0923-2508(99)00129-1","volume":"150","author":"A van Belkum","year":"1999","unstructured":"van Belkum A, van Leeuwen W, Scherer S, Verbrugh H: Occurrence and structure-function relationship of pentameric short sequence repeats in microbial genomes. Res Microbiol. 1999, 150 (9-10): 617-626. 10.1016\/S0923-2508(99)00129-1.","journal-title":"Res Microbiol"},{"key":"611_CR14","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.gene.2003.09.017","volume":"322","author":"J Yang","year":"2003","unstructured":"Yang J, Wang J, Chen L, Yu J, Dong J, Yao ZJ, Shen Y, Jin Q, Chen R: Identification and characterization of simple sequence repeats in the genomes of Shigella species. Gene. 2003, 322: 85-92. 10.1016\/j.gene.2003.09.017.","journal-title":"Gene"},{"issue":"3","key":"611_CR15","first-page":"203","volume":"4","author":"G Levinson","year":"1987","unstructured":"Levinson G, Gutman GA: Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol. 1987, 4 (3): 203-221.","journal-title":"Mol Biol Evol"},{"issue":"18","key":"611_CR16","doi-asserted-by":"publisher","first-page":"7417","DOI":"10.1093\/nar\/17.18.7417","volume":"17","author":"LW Coggins","year":"1989","unstructured":"Coggins LW, O'Prey M: DNA tertiary structures formed in vitro by misaligned hybridization of multiple tandem repeat sequences. Nucleic Acids Res. 1989, 17 (18): 7417-7426.","journal-title":"Nucleic Acids Res"},{"key":"611_CR17","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1146\/annurev.micro.54.1.641","volume":"54","author":"J Hacker","year":"2000","unstructured":"Hacker J, Kaper JB: Pathogenicity islands and the evolution of microbes. Annu Rev Microbiol. 2000, 54: 641-679. 10.1146\/annurev.micro.54.1.641.","journal-title":"Annu Rev Microbiol"},{"issue":"20","key":"611_CR18","doi-asserted-by":"publisher","first-page":"11121","DOI":"10.1073\/pnas.93.20.11121","volume":"93","author":"DW Hood","year":"1996","unstructured":"Hood DW, Deadman ME, Jennings MP, Bisercic M, Fleischmann RD, Venter JC, Moxon ER: DNA repeats identify novel virulence genes in Haemophilus influenzae. Proc Natl Acad Sci U S A. 1996, 93 (20): 11121-11125. 10.1073\/pnas.93.20.11121.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"611_CR19","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1046\/j.1365-2958.2000.02000.x","volume":"37","author":"NJ Saunders","year":"2000","unstructured":"Saunders NJ, Jeffries AC, Peden JF, Hood DW, Tettelin H, Rappuoli R, Moxon ER: Repeat-associated phase variable genes in the complete genome sequence of Neisseria meningitidis strain MC58. Mol Microbiol. 2000, 37 (1): 207-215. 10.1046\/j.1365-2958.2000.02000.x.","journal-title":"Mol Microbiol"},{"issue":"5","key":"611_CR20","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/S0966-842X(99)01493-6","volume":"7","author":"EV Sokurenko","year":"1999","unstructured":"Sokurenko EV, Hasty DL, Dykhuizen DE: Pathoadaptive mutations: gene loss and variation in bacterial pathogens. Trends Microbiol. 1999, 7 (5): 191-195. 10.1016\/S0966-842X(99)01493-6.","journal-title":"Trends Microbiol"},{"issue":"3","key":"611_CR21","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/S0966-842X(03)00010-6","volume":"11","author":"SJ Weissman","year":"2003","unstructured":"Weissman SJ, Moseley SL, Dykhuizen EV, Sokurenko EV: Enterobacterial adhesins and the case for studying SNPs in bacteria. Trends Microbiol. 2003, 11 (3): 115-117. 10.1016\/S0966-842X(03)00010-6.","journal-title":"Trends Microbiol"},{"issue":"5","key":"611_CR22","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.1046\/j.1365-2958.2002.03121.x","volume":"45","author":"JD Boddicker","year":"2002","unstructured":"Boddicker JD, Ledeboer NA, Jagnow J, Jones BD, Clegg S: Differential binding to and biofilm formation on, HEp-2 cells by Salmonella enterica serovar Typhimurium is dependent upon allelic variation in the fimH gene of the fim gene cluster. Mol Microbiol. 2002, 45 (5): 1255-1265. 10.1046\/j.1365-2958.2002.03121.x.","journal-title":"Mol Microbiol"},{"key":"611_CR23","first-page":"392","volume-title":"Virology and Immunity","author":"MT Parker","year":"1990","unstructured":"Parker MT: Glanders and melioidosis. Virology and Immunity. Edited by: Parker MTCLH. 1990, Philadelphia, PA , BC Decker, Inc., 392-394."},{"issue":"22","key":"611_CR24","doi-asserted-by":"publisher","first-page":"8487","DOI":"10.1073\/pnas.0602138103","volume":"103","author":"EE Smith","year":"2006","unstructured":"Smith EE, Buckley DG, Wu Z, Saenphimmachak C, Hoffman LR, D'Argenio DA, Miller SI, Ramsey BW, Speert DP, Moskowitz SM, Burns JL, Kaul R, Olson MV: Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. Proc Natl Acad Sci U S A. 2006, 103 (22): 8487-8492. 10.1073\/pnas.0602138103.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"611_CR25","first-page":"79","volume":"69","author":"GT Sharrer","year":"1995","unstructured":"Sharrer GT: The great glanders epizootic, 1861-1866: a Civil War legacy. Agric Hist. 1995, 69 (1): 79-97.","journal-title":"Agric Hist"},{"key":"611_CR26","first-page":"35","volume-title":"Biological and Toxin Weapons: Research, Development, and Use from the Middle Ages to 1945","author":"M Wheelis","year":"1999","unstructured":"Wheelis M: Biological sabotage in World War I. Biological and Toxin Weapons: Research, Development, and Use from the Middle Ages to 1945. Edited by: Geissler E and Moon JE. 1999, Oxford , Oxford University Press, 35-62."},{"issue":"15","key":"611_CR27","doi-asserted-by":"publisher","first-page":"4838","DOI":"10.1128\/JB.186.15.4838-4843.2004","volume":"186","author":"SM Rosenberg","year":"2004","unstructured":"Rosenberg SM, Hastings PJ: Adaptive point mutation and adaptive amplification pathways in the Escherichia coli Lac system: stress responses producing genetic change. J Bacteriol. 2004, 186 (15): 4838-4843. 10.1128\/JB.186.15.4838-4843.2004.","journal-title":"J Bacteriol"},{"issue":"12","key":"611_CR28","doi-asserted-by":"publisher","first-page":"e399","DOI":"10.1371\/journal.pbio.0020399","volume":"2","author":"PJ Hastings","year":"2004","unstructured":"Hastings PJ, Slack A, Petrosino JF, Rosenberg SM: Adaptive amplification and point mutation are independent mechanisms: evidence for various stress-inducible mutation mechanisms. PLoS Biol. 2004, 2 (12): e399-10.1371\/journal.pbio.0020399.","journal-title":"PLoS Biol"},{"issue":"2","key":"611_CR29","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1128\/JB.187.2.449-457.2005","volume":"187","author":"JC Layton","year":"2005","unstructured":"Layton JC, Foster PL: Error-prone DNA polymerase IV is regulated by the heat shock chaperone GroE in Escherichia coli. J Bacteriol. 2005, 187 (2): 449-457. 10.1128\/JB.187.2.449-457.2005.","journal-title":"J Bacteriol"},{"issue":"3","key":"611_CR30","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1111\/j.1365-2958.2005.04724.x","volume":"57","author":"JD Stumpf","year":"2005","unstructured":"Stumpf JD, Foster PL: Polyphosphate kinase regulates error-prone replication by DNA polymerase IV in Escherichia coli. Mol Microbiol. 2005, 57 (3): 751-761. 10.1111\/j.1365-2958.2005.04724.x.","journal-title":"Mol Microbiol"},{"key":"611_CR31","volume-title":"Microsatellites Evolution and Applications","author":"JE Eisen","year":"1998","unstructured":"Eisen JE: Mechanistic basis for microsatellite instability. Microsatellites Evolution and Applications. Edited by: Goldstein DB, Schlotterer . 1998, Oxford Press"},{"issue":"2","key":"611_CR32","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1038\/ng0695-213","volume":"10","author":"S Kang","year":"1995","unstructured":"Kang S, Jaworski A, Ohshima K, Wells RD: Expansion and deletion of CTG repeats from human disease genes are determined by the direction of replication in E. coli. Nat Genet. 1995, 10 (2): 213-218.","journal-title":"Nat Genet"},{"issue":"3","key":"611_CR33","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1038\/ng1197-298","volume":"17","author":"GM Samadashwily","year":"1997","unstructured":"Samadashwily GM, Raca G, Mirkin SM: Trinucleotide repeats affect DNA replication in vivo. Nat Genet. 1997, 17 (3): 298-304. 10.1038\/ng1197-298.","journal-title":"Nat Genet"},{"key":"611_CR34","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/1743-422X-2-16","volume":"2","author":"J Velten","year":"2005","unstructured":"Velten J, Morey KJ, Cazzonelli CI: Plant viral intergenic DNA sequence repeats with transcription enhancing activity. Virol J. 2005, 2: 16-10.1186\/1743-422X-2-16.","journal-title":"Virol J"},{"issue":"1","key":"611_CR35","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1006\/viro.1994.1458","volume":"203","author":"GR Arguello-Astorga","year":"1994","unstructured":"Arguello-Astorga GR, Guevara-Gonzalez RG, Herrera-Estrella LR, Rivera-Bustamante RF: Geminivirus replication origins have a group-specific organization of iterative elements: a model for replication. Virology. 1994, 203 (1): 90-100. 10.1006\/viro.1994.1458.","journal-title":"Virology"},{"issue":"37","key":"611_CR36","doi-asserted-by":"publisher","first-page":"13536","DOI":"10.1073\/pnas.0403844101","volume":"101","author":"T Pearson","year":"2004","unstructured":"Pearson T, Busch JD, Ravel J, Read TD, Rhoton SD, U'Ren JM, Simonson TS, Kachur SM, Leadem RR, Cardon ML, Van Ert MN, Huynh LY, Fraser CM, Keim P: Phylogenetic discovery bias in Bacillus anthracis using single-nucleotide polymorphisms from whole-genome sequencing. Proc Natl Acad Sci U S A. 2004, 101 (37): 13536-13541. 10.1073\/pnas.0403844101.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"611_CR37","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.meegid.2004.02.005","volume":"4","author":"P Keim","year":"2004","unstructured":"Keim P, Van Ert MN, Pearson T, Vogler AJ, Huynh LY, Wagner DM: Anthrax molecular epidemiology and forensics: using the appropriate marker for different evolutionary scales. Infect Genet Evol. 2004, 4 (3): 205-213. 10.1016\/j.meegid.2004.02.005.","journal-title":"Infect Genet Evol"},{"issue":"2","key":"611_CR38","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1128\/AAC.46.2.511-513.2002","volume":"46","author":"AJ Vogler","year":"2002","unstructured":"Vogler AJ, Busch JD, Percy-Fine S, Tipton-Hunton C, Smith KL, Keim P: Molecular analysis of rifampin resistance in Bacillus anthracis and Bacillus cereus. Antimicrob Agents Chemother. 2002, 46 (2): 511-513. 10.1128\/AAC.46.2.511-513.2002.","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"611_CR39","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1046\/j.1365-2958.2003.03297.x","volume":"47","author":"AL Jones","year":"2003","unstructured":"Jones AL, Needham RH, Clancy A, Knoll KM, Rubens CE: Penicillin-binding proteins in Streptococcus agalactiae: a novel mechanism for evasion of immune clearance. Mol Microbiol. 2003, 47 (1): 247-256. 10.1046\/j.1365-2958.2003.03297.x.","journal-title":"Mol Microbiol"},{"key":"611_CR40","first-page":"2.4.1","volume-title":"Current Protocols in Molecular Biology","author":"K Wilson","year":"1987","unstructured":"Wilson K: Preparation of genomic DNA from bacteria. Current Protocols in Molecular Biology. Edited by: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA and Struhl K. 1987, New York, NY , John Wiley & Sons, 2.4.1-2.4.5."},{"issue":"12","key":"611_CR41","doi-asserted-by":"publisher","first-page":"1125","DOI":"10.1016\/j.micinf.2003.07.004","volume":"5","author":"J Lopez","year":"2003","unstructured":"Lopez J, Copps J, Wilhelmsen C, Moore R, Kubay J, St-Jacques M, Halayko S, Kranendonk C, Toback S, DeShazer D, Fritz DL, Tom M, Woods DE: Characterization of experimental equine glanders. Microbes Infect. 2003, 5 (12): 1125-1131. 10.1016\/j.micinf.2003.07.004.","journal-title":"Microbes Infect"},{"issue":"3","key":"611_CR42","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1093\/bib\/5.3.237","volume":"5","author":"M Pop","year":"2004","unstructured":"Pop M, Phillippy A, Delcher AL, Salzberg SL: Comparative genome assembly. Brief Bioinform. 2004, 5 (3): 237-248. 10.1093\/bib\/5.3.237.","journal-title":"Brief Bioinform"},{"issue":"2","key":"611_CR43","doi-asserted-by":"publisher","first-page":"R12","DOI":"10.1186\/gb-2004-5-2-r12","volume":"5","author":"S Kurtz","year":"2004","unstructured":"Kurtz S, Phillippy A, Delcher AL, Smoot M, Shumway M, Antonescu C, Salzberg SL: Versatile and open software for comparing large genomes. Genome Biol. 2004, 5 (2): R12-10.1186\/gb-2004-5-2-r12.","journal-title":"Genome Biol"},{"issue":"6","key":"611_CR44","first-page":"671","volume":"10","author":"SW Smith","year":"1994","unstructured":"Smith SW, Overbeek R, Woese CR, Gilbert W, Gillevet PM: The genetic data environment an expandable GUI for multiple sequence analysis. Comput Appl Biosci. 1994, 10 (6): 671-675.","journal-title":"Comput Appl Biosci"},{"key":"611_CR45","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1089\/gst.1995.1.9","volume":"1","author":"GG Suttin","year":"1995","unstructured":"Suttin GG, White O, Adams MD, Kerlavage AR: TIGR Assembler: A new tool for assembling large shotgun sequencing projects. Genome Science and Technology. 1995, 1: 9-19.","journal-title":"Genome Science and Technology"}],"container-title":["BMC Genomics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2164-7-228.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1471-2164-7-228\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2164-7-228.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T01:53:24Z","timestamp":1630461204000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcgenomics.biomedcentral.com\/articles\/10.1186\/1471-2164-7-228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9,5]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["611"],"URL":"https:\/\/doi.org\/10.1186\/1471-2164-7-228","relation":{},"ISSN":["1471-2164"],"issn-type":[{"value":"1471-2164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9,5]]},"assertion":[{"value":"17 April 2006","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 September 2006","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 September 2006","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"228"}}