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However, unlike protein genes, no simple feature enables the prediction of the location of the corresponding sequences in genomes. Several approaches, of variable usefulness, to identify genomic sequences encoding sRNA have been described in recent years.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We used a combination of <jats:italic>in silico<\/jats:italic> comparative genomics and microarray-based transcriptional profiling. This approach to screening identified ~60 intergenic regions conserved between <jats:italic>Sinorhizobium meliloti<\/jats:italic> and related members of the alpha-proteobacteria sub-group 2. Of these, 14 appear to correspond to novel non-coding sRNAs and three are putative peptide-coding or 5' UTR RNAs (ORF smaller than 100 aa). The expression of each of these new small RNA genes was confirmed by Northern blot hybridization.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Small non coding RNA (<jats:italic>sra<\/jats:italic>) genes can be found in the intergenic regions of alpha-proteobacteria genomes. Some of these <jats:italic>sra<\/jats:italic> genes are only present in <jats:italic>S. meliloti<\/jats:italic>, sometimes in genomic islands; homologues of others are present in related genomes including those of the pathogens <jats:italic>Brucella<\/jats:italic> and <jats:italic>Agrobacterium<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2164-8-467","type":"journal-article","created":{"date-parts":[[2007,12,19]],"date-time":"2007-12-19T19:14:14Z","timestamp":1198091654000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Identification of chromosomal alpha-proteobacterial small RNAs by comparative genome analysis and detection in Sinorhizobium meliloti strain 1021"],"prefix":"10.1186","volume":"8","author":[{"given":"Vincent M","family":"Ulv\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emeric W","family":"Sevin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lique","family":"Ch\u00e9ron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9rique","family":"Barloy-Hubler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,12,19]]},"reference":[{"key":"1180_CR1","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1038\/35103511","volume":"2","author":"SR Eddy","year":"2001","unstructured":"Eddy SR: Non-coding RNA genes and the modern RNA world. 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