{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T18:56:43Z","timestamp":1774119403968,"version":"3.50.1"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Immunol"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>An accurate test for <jats:italic>Mycobacterium tuberculosis<\/jats:italic> infection is urgently needed in immunosuppressed populations. The aim of this study was to investigate the diagnostic power of enzyme-linked immunospot (ELISPOT)-based IFN-\u03b3 release assay in detecting active and latent tuberculosis in HIV-infected population in <jats:italic>bacillus Calmette-Guerin<\/jats:italic> (BCG)-vaccinated area. A total of 100 HIV-infected individuals including 32 active tuberculosis patients were recruited. An ELISPOT-based IFN-\u03b3 release assay, T-SPOT.TB, was used to evaluate the <jats:italic>M. tuberculosis<\/jats:italic> ESAT-6 and CFP-10 specific IFN-\u03b3 response. Tuberculin skin test (TST) was performed for all recruited subjects.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The subjects were divided into group HIV+ATB (HIV-infected individuals with active tuberculosis, n = 32), group HIV+LTB (HIV-infected individuals with positive results of T-SPOT.TB assay, n = 46) and group HIV only (HIV-infected individuals with negative results of T-SPOT.TB assay and without evidence of tuberculosis infection, n = 22). In group HIV+ATB and HIV+LTB, T-SPOT.TB positive rate in subjects with TST &lt;5 mm were 50% (16\/32) and 41.3% (19\/46), respectively. Individuals in group HIV+ATB and HIV+LTB with CD4+ T cells &lt;500\/\u03bcl, T-SPOT.TB showed a higher sensitivity than TST (64.5% vs. 22.6% and 62.2% vs. 29.7%, respectively, both <jats:italic>P<\/jats:italic> &lt; 0.0001). In addition, the sensitivity of T-SPOT.TB assay in group HIV+ATB increased to &gt;85% in patients with TB treatment for less than 1 month and CD4+ T cells \u2265200\/\u03bcl, while for patients treated for more than 3 months and CD4+ T cells &lt;200\/\u03bcl, the sensitivity was decreased to only 33.3%. Furthermore, the results could be generated by T-SPOT.TB assay within 24 hours, which was more rapid than TST with 48\u201372 hours.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>ELISPOT-based IFN-\u03b3 release assay is more sensitive and rapid for the diagnosis of TB infection in Chinese HIV-infected individuals with history of BCG vaccination, and could be an effective tool for guiding preventive treatment with isoniazid in latently infected people and for TB control in China.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2172-10-31","type":"journal-article","created":{"date-parts":[[2009,5,28]],"date-time":"2009-05-28T19:17:50Z","timestamp":1243538270000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["High-sensitive and rapid detection of Mycobacterium tuberculosis infection by IFN-\u03b3 release assay among HIV-infected individuals in BCG-vaccinated area"],"prefix":"10.1186","volume":"10","author":[{"given":"Weimin","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyun","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyan","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunya","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingli","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinhua","family":"Weng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2009,5,28]]},"reference":[{"key":"251_CR1","volume-title":"WHO report","author":"WHO","year":"2008","unstructured":"WHO: Global tuberculosis control- surveillance, planning, financing. 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