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Here, we describe a study that leads to the identification of a novel HLA-A*0201-restricted epitope from conserved regions of S protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>First, different SARS-CoV sequences were analyzed to predict eight candidate peptides from conserved regions of the S protein based upon HLA-A*0201 binding and proteosomal cleavage. Four of eight candidate peptides were tested by HLA-A*0201 binding assays. Among the four candidate peptides, Sp8 (S<jats:sub>958-966<\/jats:sub>, VLNDILSRL) induced specific CTLs both<jats:italic>ex vivo<\/jats:italic>in PBLs of healthy HLA-A2<jats:sup>+<\/jats:sup>donors and in HLA-A2.1\/K<jats:sup>b<\/jats:sup>transgenic mice immunized with a plasmid encoding full-length S protein. The immunized mice released IFN-\u03b3 and lysed target cells upon stimulation with Sp8 peptide-pulsed autologous dendritic cells in comparison to other candidates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that Sp8 is a naturally processed epitope. We propose that Sp8 epitope should help in the characterization of mechanisms of virus control and immunopathology in SARS-CoV infection.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2172-10-61","type":"journal-article","created":{"date-parts":[[2009,12,3]],"date-time":"2009-12-03T20:24:15Z","timestamp":1259871855000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Identification of a novel conserved HLA-A*0201-restricted epitope from the spike protein of SARS-CoV"],"prefix":"10.1186","volume":"10","author":[{"given":"Yanbo","family":"Lv","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihua","family":"Ruan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuzhang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2009,12,3]]},"reference":[{"key":"281_CR1","doi-asserted-by":"publisher","first-page":"1967","DOI":"10.1056\/NEJMoa030747","volume":"348","author":"C Drosten","year":"2003","unstructured":"Drosten C, Gunther S, Preiser W, Werf van der S, Brodt HR, Becker S, Rabenau H, Panning M, Kolesnikova L, Fouchier RA, et al.: Identification of a novel coronavirus in patients with severe acute respiratory syndrome. 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