{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T05:24:05Z","timestamp":1748237045146},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Immunol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>We previously showed that co-immunization with a protein antigen and a DNA vaccine coding for the same antigen induces CD40<jats:sup>low<\/jats:sup> IL-10<jats:sup>high<\/jats:sup> tolerogenic DCs, which in turn stimulates the expansion of antigen-specific CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>-<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup> regulatory T cells (CD25<jats:sup>-<\/jats:sup> iTreg). However, it was unclear how to choose the antigen sequence to maximize tolerogenic antigen presentation and, consequently, CD25<jats:sup>-<\/jats:sup> iTreg induction.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In the present study, we demonstrated the requirement of highly antigenic epitopes for CD25<jats:sup>-<\/jats:sup> iTreg induction. Firstly, we showed that the induction of CD25<jats:sup>-<\/jats:sup> iTreg by tolerogenic DC can be blocked by anti-MHC-II antibody. Next, both the number and the suppressive activity of CD25<jats:sup>-<\/jats:sup> iTreg correlated positively with the overt antigenicity of an epitope to activate T cells. Finally, in a mouse model of dermatitis, highly antigenic epitopes derived from a flea allergen not only induced more CD25<jats:sup>-<\/jats:sup> iTreg, but also more effectively prevented allergenic reaction to the allergen than did weakly antigenic epitopes.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Our data thus indicate that efficient induction of CD25<jats:sup>-<\/jats:sup> iTreg requires highly antigenic peptide epitopes. This finding suggests that highly antigenic epitopes should be used for efficient induction of CD25<jats:sup>-<\/jats:sup> iTreg for clinical applications such as flea allergic dermatitis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2172-12-27","type":"journal-article","created":{"date-parts":[[2011,5,5]],"date-time":"2011-05-05T06:17:35Z","timestamp":1304576255000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Efficient induction of CD25- iTreg by co-immunization requires strongly antigenic epitopes for T cells"],"prefix":"10.1186","volume":"12","author":[{"given":"Shuang","family":"Geng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youmin","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Pavlakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huali","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinyao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanxin","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weibin","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,5,5]]},"reference":[{"key":"375_CR1","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1097\/01.tp.0000251808.91901.c3","volume":"83","author":"S Aiello","year":"2007","unstructured":"Aiello S, Cassis P, Cassis L, Tomasoni S, Benigni A, Pezzotta A, Cavinato RA, Cugini D, Azzollini N, Mister M, et al: DnIKK2-transfected dendritic cells induce a novel population of inducible nitric oxide synthase-expressing CD4+CD25- cells with tolerogenic properties. 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