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Thus, site-specific differences in phenotypes of mucosal and systemic T cell populations have been observed.<jats:italic>Chlamydia trachomatis<\/jats:italic>most commonly infects the endocervix in women, yet little is known about<jats:italic>Chlamydia<\/jats:italic>-specific effector T cell immunity at this unique mucosal site. Our previous flow-cytometry-based study of cervical-cytobrush retrieved cells indicated that CD8 T cells are significantly increased in the<jats:italic>C. trachomatis<\/jats:italic>-infected human endocervix. The cytolytic function of CD8 T cells is important in the protective immunity against many intracellular pathogens, and requires the cytolytic granule perforin to facilitate the entry of other molecules that mediate the lysis of target cells. Determination of perforin expression of the CD8 T cell population in the endocervix would therefore provide insights on the granule-mediated cytolytic potential of these cells at this site.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our histological data revealed that<jats:italic>C. trachomatis<\/jats:italic>-infected tissues have significantly higher numbers of CD3 and CD8 T cells compared to non-infected tissues (p&lt;0.01), and that the majority of CD8<jats:sup>+<\/jats:sup>cells do not express perforin<jats:italic>in situ<\/jats:italic>. A subsequent flow cytometric analysis of paired blood and endocervix-derived cells (n=16) revealed that while all the CD8 T cell subsets: na\u00efve, effector memory (T<jats:sub>EM<\/jats:sub>), central memory (T<jats:sub>CM<\/jats:sub>) and terminally differentiated effector memory (T<jats:sub>EMRA<\/jats:sub>) can be found in the blood, the endocervix is populated mainly by the T<jats:sub>EM<\/jats:sub>CD8 T cell subset. Our data also showed that perforin expression in the T<jats:sub>EM<\/jats:sub>population is significantly lower in the endocervix than in the blood of<jats:italic>C. trachomatis<\/jats:italic>positive women (n=15; p&lt;0.0001), as well as in<jats:italic>C. trachomatis<\/jats:italic>-negative individuals (n=6; p&lt;0.05). Interestingly, our<jats:italic>in vitro<\/jats:italic>co-culture study suggests that the exposure of HeLa 229 cervical epithelial cells to IFN gamma could potentially induce a decrease in perforin content in CD8 T<jats:sub>EM<\/jats:sub>cells in the same microenvironment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The low perforin content of CD8 T<jats:sub>EM<\/jats:sub>cells in the endocervix, the local site of<jats:italic>C. trachomatis<\/jats:italic>infection in women, may reflect the unique immunological environment that balances immune protection against sexually transmitted infections and immune- tolerance to support conception.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2172-13-66","type":"journal-article","created":{"date-parts":[[2012,12,8]],"date-time":"2012-12-08T00:28:36Z","timestamp":1354926516000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The major CD8 T cell effector memory subset in the normal and Chlamydia trachomatis-infected human endocervix is low in perforin"],"prefix":"10.1186","volume":"13","author":[{"given":"Joyce A","family":"Ibana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leann","family":"Myers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constance","family":"Porretta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie N","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison J","family":"Quayle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,12,7]]},"reference":[{"issue":"4","key":"484_CR1","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/0167-5699(95)80122-7","volume":"16","author":"MJ Smyth","year":"1995","unstructured":"Smyth MJ, Trapani JA: Granzymes: exogenous proteinases that induce target cell apoptosis. 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