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The aim of this study was to investigate the expression of TLR2 and TLR9 in jejunum and colon and its correlation with CD11c, CD11b, and CD14 receptors used as markers for dendritic cells and macrophages.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Twenty four dogs infected with <jats:italic>Leishmania infantum<\/jats:italic> were used in this study. Cytometry was carried out in lamina propria cells from jejunum and colon using markers for TLR2, TLR9, CD11b, CD11c and CD14.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Cellular inflammatory exudate was diffuse in the mucosa and submucosa, predominately comprising mononuclear cells: plasma cells, macrophages, and lymphocytes. Despite the parasite load, microscopy showed no erosion was evident in the epithelial mucosa layers. The colon harbored more parasites than the jejunum. Flow cytometry revealed higher frequency of TLR2<jats:sup>+<\/jats:sup> and CD11c<jats:sup>+<\/jats:sup> dendritic cells in the colon than in the jejunum. Conversely, TLR9-expressing cells were more frequent in jejunum. Moreover, frequency of macrophages (CD11b<jats:sup>+<\/jats:sup> and CD14<jats:sup>+<\/jats:sup>) expressing simultaneity TLR9 were lower in the colon than in jejunum, while CD11c<jats:sup>+<\/jats:sup> cells predominated in the colon. Despite of the negative ELISA serum results, IL-10 and TNF-\u03b1 were higher in jejunum than colon of infected animals. However, IL-4 was higher in colon than jejunum of infected animals. A higher expression these cytokines were demonstrated in infected dogs compared to uninfected dogs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>There was no correlation between clinical signs and pathological changes and immunological and parasitological findings in the gastrointestinal tract in canine visceral leishmaniasis. However, jejunum showed a lower parasite load with increased frequency and expression of CD11b, TLR9, CD14\/CD11b\/TLR9 receptors and IL-10 and TNF-\u03b1 cytokines. Conversely, the colon showed a higher parasite load along with increased frequency and expression of TLR2, CD11c receptors, and IL-4 cytokine. Thus, <jats:italic>Leishmania infantum<\/jats:italic> is able to interfere in jejunum increased expression of TLR2, TLR9, CD11b, CD14, CD14\/CD11b\/TLR9 receptors, IL-10, and TNF-\u03b1; and in colon increased expression of CD11c, TLR2, TLR9, CD11b, CD14 e, CD14\/CD11b\/TLR9 receptors, IL-10, and TNF-\u03b1.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2172-14-22","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:14:25Z","timestamp":1368490465000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum"],"prefix":"10.1186","volume":"14","author":[{"given":"Maria M","family":"Figueiredo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izabela FG","family":"Amorim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldair JW","family":"Pinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edtor S","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc\u00e9lia","family":"de Jesus Pinheiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Deoti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana MC","family":"Faria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagner L","family":"Tafuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,5,14]]},"reference":[{"key":"530_CR1","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1186\/1751-0147-53-67","volume":"53","author":"AJ Pinto","year":"2011","unstructured":"Pinto AJ, Figueiredo MM, Silva FL, Martins T, Michalick MS, Tafuri WL: Histopathological and parasitological study of the gastrointestinal tract of dogs naturally infected with Leishmania infantum. 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