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The GST-tagged full length FliI protein was shown to possess ATPase activity, hydrolyzing ATP at a rate of 0.15 \u00b1 .02 \u03bcmol min<jats:sup>-1<\/jats:sup> mg<jats:sup>-1<\/jats:sup> in a time- and dose-dependant manner. Using bacterial-2-hybrid and GST pull-down assays, the N-terminal domain of FliI was shown to interact with the cytoplasmic domain of FlhA, but not with FliF, and the cytoplasmic domain of FlhA was shown to interact with the C-terminus of FliF. The absence of other flagellar orthologs led us to explore cross-reaction of flagellar proteins with type III secretion proteins, and we found that FliI interacted with CdsL and CopN, while FlhA interacted with CdsL and Cpn0322 (YscU ortholog CdsU).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The specific interaction of the four orthologous flagellar proteins in <jats:italic>C. pneumoniae<\/jats:italic> suggests that they interact <jats:italic>in vivo<\/jats:italic> and, taken together with their conservation across members of the chlamydiae <jats:italic>sps<\/jats:italic>., and their interaction with T3S components, suggests a role in bacterial replication and\/or intracellular survival.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-10-18","type":"journal-article","created":{"date-parts":[[2010,1,22]],"date-time":"2010-01-22T19:15:49Z","timestamp":1264187749000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Interactions between flagellar and type III secretion proteins in Chlamydia pneumoniae"],"prefix":"10.1186","volume":"10","author":[{"given":"Chris B","family":"Stone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C","family":"Bulir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jodi D","family":"Gilchrist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raman K","family":"Toor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James B","family":"Mahony","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,1,22]]},"reference":[{"key":"974_CR1","doi-asserted-by":"publisher","first-page":"e66","DOI":"10.2741\/hahn","volume":"7","author":"D Hahn","year":"2002","unstructured":"Hahn D, Azenabor A, Beatty W, Byrne G: Chlamydia pneumoniae as a respiratory pathogen. 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