{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T14:06:40Z","timestamp":1793801200161,"version":"4.6.1"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Phospholipase B (PLB) has been reported to be one of the virulence factors for human pathogenic fungi and has also been described as necessary for the early events in infection. Based on these data, we investigated the role of PLB in virulence and modulation of the alveolar pulmonary immune response during infection using an <jats:italic>in-vitro<\/jats:italic> model of host-pathogen interaction, i.e. <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> yeast cells infecting alveolar macrophage (MH-S) cells.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The effect of PLB was analyzed using the specific inhibitor alexidine dihydrochloride (0.25 \u03bcM), and pulmonary surfactant (100 \u03bcg mL<jats:sup>-1<\/jats:sup>), during 6 hours of co-cultivation of <jats:italic>P. brasiliensis<\/jats:italic> and MH-S cells. Alexidine dihydrochloride inhibited PLB activity by 66% and significantly decreased the adhesion and internalization of yeast cells by MH-S cells. Genes involved in phagocytosis (<jats:italic>trl2<\/jats:italic>, <jats:italic>cd14<\/jats:italic>) and the inflammatory response (<jats:italic>nfkb, tnf-\u03b1, il-1\u03b2<\/jats:italic>) were down-regulated in the presence of this PLB inhibitor. In contrast, PLB activity and internalization of yeast cells significantly increased in the presence of pulmonary surfactant; under this condition, genes such as <jats:italic>clec<\/jats:italic> 2 and the pro-inflammatory inhibitor (<jats:italic>nkrf<\/jats:italic>) were up-regulated. Also, the pulmonary surfactant did not alter cytokine production, while alexidine dihydrochloride decreased the levels of interleukin-10 (IL-10) and increased the levels of IL-12 and tumor necrosis factor-\u03b1 (TNF-\u03b1). In addition, gene expression analysis of <jats:italic>plb1<\/jats:italic>, s<jats:italic>od3<\/jats:italic> and <jats:italic>icl1<\/jats:italic> suggests that <jats:italic>P. brasiliensis<\/jats:italic> gene re-programming is effective in facilitating adaptation to this inhospitable environment, which mimics the lung-environment interaction.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>\n              <jats:italic>P. brasiliensis<\/jats:italic> PLB activity is involved in the process of adhesion and internalization of yeast cells at the MH-S cell surface and may enhance virulence and subsequent down-regulation of macrophage activation.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-10-241","type":"journal-article","created":{"date-parts":[[2010,9,16]],"date-time":"2010-09-16T06:13:55Z","timestamp":1284617635000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction"],"prefix":"10.1186","volume":"10","author":[{"given":"Deyze Alencar","family":"Soares","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ros\u00e2ngela Vieira","family":"de Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simoneide Sousa","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anam\u00e9lia Lorenzetti","family":"Bocca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sueli Maria","family":"Soares Felipe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Petrofeza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,9,15]]},"reference":[{"key":"1197_CR1","first-page":"205","volume-title":"Fungal pathogenesis: principles and clinical applications","author":"G San-Blas","year":"2001","unstructured":"San-Blas G, Nino-Vega G: Paracoccidioides brasiliensis: virulence and host response. 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