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Transmission of pathogenic <jats:italic>Leptospira<\/jats:italic> to humans mostly occurs through abraded skin or mucosal surfaces after direct or indirect contact with infected animals or contaminated soil or water. The spirochete then spreads hematogenously, resulting in multi-organ failure and death in severe cases. Previous DNA microarray studies have identified differentially expressed genes required for adaptation to temperature and osmolarity conditions inside the host compared to those of the environment.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In order to identify genes involved in survival in the early spirochetemic phase of infection, we performed a transcriptional analysis of <jats:italic>L. interrogans<\/jats:italic> serovar Copenhageni upon exposure to serum in comparison with EMJH medium. One hundred and sixty-eight genes were found to be differentially expressed, of which 55 were up-regulated and 113 were down-regulated. Genes of known or predicted function accounted for 54.5 and 45.1% of up- and down-regulated genes, respectively. Most of the differentially expressed genes were predicted to be involved in transcriptional regulation, translational process, two-component signal transduction systems, cell or membrane biogenesis, and metabolic pathways.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Our study showed global transcriptional changes of pathogenic <jats:italic>Leptospira<\/jats:italic> upon exposure to serum, representing a specific host environmental cue present in the bloodstream. The presence of serum led to a distinct pattern of gene expression in comparison to those of previous single-stimulus microarray studies on the effect of temperature and osmolarity upshift. The results provide insights into the pathogenesis of leptospirosis during the early bacteremic phase of infection.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-10-31","type":"journal-article","created":{"date-parts":[[2010,1,30]],"date-time":"2010-01-30T07:14:56Z","timestamp":1264835696000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Global transcriptomic response of Leptospira interrogans serovar Copenhageni upon exposure to serum"],"prefix":"10.1186","volume":"10","author":[{"given":"Kanitha","family":"Patarakul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miranda","family":"Lo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Adler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,1,29]]},"reference":[{"key":"987_CR1","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","volume":"3","author":"AR Bharti","year":"2003","unstructured":"Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM: Leptospirosis: a zoonotic disease of global importance. 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