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In the present study, we characterized the impact of one month intake of a synbiotic food, containing fructooligosaccharides and the probiotic strains <jats:italic>Lactobacillus helveticus<\/jats:italic> Bar13 and <jats:italic>Bifidobacterium longum<\/jats:italic> Bar33, on the gut microbiota composition and metabolic profiles of 20 healthy subjects.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The synbiotic food did not modify the overall structure of the gut microbiome, as indicated by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The ability of the probiotic <jats:italic>L. helveticus<\/jats:italic> and <jats:italic>B. longum<\/jats:italic> strains to pass through the gastrointestinal tract was hypothesized on the basis of real-time PCR data. In spite of a stable microbiota, the intake of the synbiotic food resulted in a shift of the fecal metabolic profiles, highlighted by the Gas Chromatography Mass Spectrometry Solid Phase Micro-Extraction (GC-MS\/SPME) analysis. The extent of short chain fatty acids (SCFA), ketones, carbon disulfide and methyl acetate was significantly affected by the synbiotic food consumption. Furthermore, the Canonical discriminant Analysis of Principal coordinates (CAP) of GC-MS\/SPME profiles allowed a separation of the stool samples recovered before and after the consumption of the functional food.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>In this study we investigated the global impact of a dietary intervention on the gut ecology and metabolism in healthy humans. We demonstrated that the intake of a synbiotic food leads to a modulation of the gut metabolic activities with a maintenance of the gut biostructure. In particular, the significant increase of SCFA, ketones, carbon disulfide and methyl acetate following the feeding period suggests potential health promoting effects of the synbiotic food.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-10-4","type":"journal-article","created":{"date-parts":[[2010,1,7]],"date-time":"2010-01-07T19:31:50Z","timestamp":1262892710000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":136,"title":["Impact of a synbiotic food on the gut microbial ecology and metabolic profiles"],"prefix":"10.1186","volume":"10","author":[{"given":"Beatrice","family":"Vitali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurice","family":"Ndagijimana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Cruciani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Carnevali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Candela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elisabetta","family":"Guerzoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Brigidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,1,7]]},"reference":[{"key":"960_CR1","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1021\/pr060522z","volume":"6","author":"S Rezzi","year":"2007","unstructured":"Rezzi S, Ramadan Z, Fay LB, Kochhar S: Nutritional metabonomics: applications and perspectives. 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