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ClpP, the catalytic core of the Clp proteolytic complex, is widely involved in many cellular processes via the regulation of intracellular protein quality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this study, we showed that ClpP was required for optimal growth of<jats:italic>L. pneumophila<\/jats:italic>at high temperatures and under several other stress conditions. We also observed that cells devoid of<jats:italic>clpP<\/jats:italic>exhibited cell elongation, incomplete cell division and compromised colony formation. Furthermore, we found that the<jats:italic>clpP<\/jats:italic>-deleted mutant was more resistant to sodium stress and failed to proliferate in the amoebae host<jats:italic>Acanthamoeba castellanii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The data present in this study illustrate that the ClpP protease homologue plays an important role in the expression of transmission traits and cell division of<jats:italic>L. pneumophila<\/jats:italic>, and further suggest a putative role of ClpP in virulence regulation.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2180-10-54","type":"journal-article","created":{"date-parts":[[2010,2,19]],"date-time":"2010-02-19T07:15:08Z","timestamp":1266563708000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["The ClpP protease homologue is required for the transmission traits and cell division of the pathogen Legionella pneumophila"],"prefix":"10.1186","volume":"10","author":[{"given":"Xiang-hui","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-lun","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-cong","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin-fen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-ning","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-jun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,2,19]]},"reference":[{"key":"1010_CR1","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1056\/NEJM197712012972201","volume":"297","author":"DW Fraser","year":"1977","unstructured":"Fraser DW, Tsai TR, Orenstein W, Parkin WE, Beecham HJ, Sharrar RG, Harris J, Mallison GF, Martin SM, McDade JE, Shepard CC, Brachman PS: Legionnaires' disease: description of an epidemic of pneumonia. 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