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In recent years,\n                      <jats:italic>Filifactor alocis<\/jats:italic>\n                      , a fastidious, Gram-positive, obligately anaerobic rod was repeatedly identified in periodontal lesions using DNA-based methods. It has been suggested to be a marker for periodontal deterioration. The present study investigated the epidemiology of\n                      <jats:italic>F. alocis<\/jats:italic>\n                      in periodontal pockets and analysed the spatial arrangement and architectural role of the organism in\n                      <jats:italic>in vivo<\/jats:italic>\n                      grown subgingival biofilms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A species-specific oligonucleotide probe, FIAL, was designed and evaluated. A total of 490 subgingival plaque samples were submitted to PCR and subsequent dot blot hybridization to compare the prevalence of\n                      <jats:italic>F. alocis<\/jats:italic>\n                      in patients suffering from generalized aggressive periodontitis (GAP), chronic periodontitis (CP), and control subjects resistant to periodontitis. Moreover, a specially designed carrier system was used to collect\n                      <jats:italic>in vivo<\/jats:italic>\n                      grown subgingival biofilms from GAP patients. Subsequent topographic analysis was performed using fluorescence in situ hybridization.\n                    <\/jats:p>\n                    <jats:p>\n                      While the majority of patients suffering from GAP or CP harboured\n                      <jats:italic>F. alocis<\/jats:italic>\n                      , it was rarely detected in the control group. In the examined carrier-borne biofilms the organism predominantly colonized apical parts of the pocket in close proximity to the soft tissues and was involved in numerous structures that constitute characteristic architectural features of subgingival periodontal biofilms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>F. alocis<\/jats:italic>\n                      is likely to make a relevant contribution to the pathogenetic structure of biofilms accounting for periodontal inflammation and can be considered an excellent marker organism for periodontal disease.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/1471-2180-10-66","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T12:04:50Z","timestamp":1340885090000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":111,"title":["Filifactor alocis - involvement in periodontal biofilms"],"prefix":"10.1186","volume":"10","author":[{"given":"Sebastian","family":"Schlafer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Riep","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann L","family":"Griffen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annett","family":"Petrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"H\u00fcbner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz","family":"Berning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Friedmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf B","family":"G\u00f6bel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Moter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,3,1]]},"reference":[{"key":"1022_CR1","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1111\/j.1600-0757.1994.tb00020.x","volume":"5","author":"AD Haffajee","year":"1994","unstructured":"Haffajee AD, Socransky SS: Microbial etiological agents of destructive periodontal diseases. 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