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Resistance to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and superoxides has been extensively studied in <jats:italic>S. meliloti<\/jats:italic>. In contrast resistance to organic peroxides has not been investigated while <jats:italic>S. meliloti<\/jats:italic> genome encodes putative organic peroxidases. Organic peroxides are produced by plants and are highly toxic. The resistance to these oxygen radicals has been studied in various bacteria but never in plant nodulating bacteria.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In this study we report the characterisation of organic hydroperoxide resistance gene <jats:italic>ohr<\/jats:italic> and its regulator <jats:italic>ohrR<\/jats:italic> in <jats:italic>S. meliloti<\/jats:italic>. The inactivation of <jats:italic>ohr<\/jats:italic> affects resistance to cumene and ter-butyl hydroperoxides but not to hydrogen peroxide or menadione <jats:italic>in vitro<\/jats:italic>. The expression of <jats:italic>ohr<\/jats:italic> and <jats:italic>ohrR<\/jats:italic> genes is specifically induced by organic peroxides. OhrR binds to the intergenic region between the divergent genes <jats:italic>ohr<\/jats:italic> and <jats:italic>ohrR<\/jats:italic>. Two binding sites were characterised. Binding to the operator is prevented by OhrR oxidation that promotes OhrR dimerisation. The inactivation of <jats:italic>ohr<\/jats:italic> did not affect symbiosis and nitrogen fixation, suggesting that redundant enzymatic activity exists in this strain. Both <jats:italic>ohr<\/jats:italic> and <jats:italic>ohrR<\/jats:italic> are expressed in nodules suggesting that they play a role during nitrogen fixation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This report demonstrates the significant role Ohr and OhrR proteins play in bacterial stress resistance against organic peroxides in <jats:italic>S. meliloti<\/jats:italic>. The <jats:italic>ohr<\/jats:italic> and <jats:italic>ohrR<\/jats:italic> genes are expressed in nodule-inhabiting bacteroids suggesting a role during nodulation.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-11-100","type":"journal-article","created":{"date-parts":[[2011,5,13]],"date-time":"2011-05-13T18:17:39Z","timestamp":1305310659000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Resistance to organic hydroperoxides requires ohr and ohrR genes in Sinorhizobium meliloti"],"prefix":"10.1186","volume":"11","author":[{"given":"Catherine","family":"Fontenelle","sequence":"first","affiliation":[]},{"given":"Carlos","family":"Blanco","sequence":"additional","affiliation":[]},{"given":"Morgane","family":"Arrieta","sequence":"additional","affiliation":[]},{"given":"Virginie","family":"Dufour","sequence":"additional","affiliation":[]},{"given":"Annie","family":"Trautwetter","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,5,13]]},"reference":[{"key":"1374_CR1","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1186\/1471-2180-10-192","volume":"10","author":"C Fernandez-Aunion","year":"2010","unstructured":"Fernandez-Aunion C, Hamouda TB, Iglesias-Guerra F, Argandona M, Reina-Bueno M, Nieto JJ, Aouani ME, Vargas C: Biosynthesis of compatible solutes in rhizobial strains isolated from Phaseolus vulgaris nodules in Tunisian fields. 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