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Besides production of Shiga toxins, the presence of LEE (\n                      <jats:italic>eae<\/jats:italic>\n                      -gene) and non-LEE (\n                      <jats:italic>nle<\/jats:italic>\n                      ) encoded effector genes harboured on O-islands OI-122, OI-71 and OI-57 is associated with EHEC virulence and their frequency in outbreaks. Genes encoded by the EHEC-plasmid are putative virulence markers of EHEC. EHEC-plasmids, LEE and non-LEE effector genes have also been detected in some strains of enteropathogenic\n                      <jats:italic>E. coli<\/jats:italic>\n                      (EPEC). The objective of this study was to analyze the relationship between EHEC and EPEC for virulence genes encoded by genomic O-islands and by the EHEC-plasmids.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Nle genes\n                      <jats:italic>ent\/espL2<\/jats:italic>\n                      ,\n                      <jats:italic>nleB<\/jats:italic>\n                      and\n                      <jats:italic>nleE<\/jats:italic>\n                      (OI-122),\n                      <jats:italic>nleA<\/jats:italic>\n                      ,\n                      <jats:italic>nleF<\/jats:italic>\n                      and\n                      <jats:italic>nleH1-2<\/jats:italic>\n                      (OI-71),\n                      <jats:italic>nleG5-2<\/jats:italic>\n                      and\n                      <jats:italic>nleG6-2<\/jats:italic>\n                      (OI-57),\n                      <jats:italic>espK<\/jats:italic>\n                      (CP-933N) and the EHEC-plasmid encoded genes\n                      <jats:italic>ehxA<\/jats:italic>\n                      ,\n                      <jats:italic>espP<\/jats:italic>\n                      ,\n                      <jats:italic>etpD<\/jats:italic>\n                      and\n                      <jats:italic>katP<\/jats:italic>\n                      were searched in 73 typical and in 235 atypical enteropathogenic\n                      <jats:italic>E. coli<\/jats:italic>\n                      (EPEC) strains. Typical and atypical EPEC each fall into two clusters. Cluster 1 typical (n = 46) and atypical (n = 129) EPEC strains were characterized by the presence of OI-122 encoded genes and grouped together with 64 investigated EHEC strains. Cluster 2 typical (n = 27) and atypical (n = 106) strains grouped together with 52 LEE-negative, Shiga toxin-producing\n                      <jats:italic>E. coli<\/jats:italic>\n                      (STEC) and with 21 apathogenic\n                      <jats:italic>E. coli<\/jats:italic>\n                      strains. Typical EPEC Cluster 1 strains belonged to serotypes frequently involved in severe illness and outbreaks in children (O111:H2, O114:H2, O55:H6, O127:H6 and O142:H6). Atypical EPEC Cluster 1 strains were characterized by serotypes related to EHEC (O26:H11, O55:H7, O145:H28, O103:H2 and O103:H25).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The OI-122 encoded\n                      <jats:italic>nleB<\/jats:italic>\n                      gene was found to be most closely associated with Cluster 1 strains and may serve as a diagnostic tool for the identification of virulent EHEC and EPEC seropathotypes. OI-71 encoded genes\n                      <jats:italic>nleA<\/jats:italic>\n                      ,\n                      <jats:italic>nleF<\/jats:italic>\n                      and\n                      <jats:italic>nleH1-2<\/jats:italic>\n                      are less associated with Cluster 1 strains. EHEC-plasmid, OI-57 and CP-933 associated genes showed only weak similarities with virulent Cluster 1 EHEC and EPEC strains.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/1471-2180-11-142","type":"journal-article","created":{"date-parts":[[2011,6,21]],"date-time":"2011-06-21T14:14:14Z","timestamp":1308665654000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":108,"title":["Virulence gene profiling of enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains: a basis for molecular risk assessment of typical and atypical EPEC strains"],"prefix":"10.1186","volume":"11","author":[{"given":"Marie","family":"Bugarel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annett","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Fach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lothar","family":"Beutin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,6,21]]},"reference":[{"key":"1419_CR1","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1172\/JCI12404","volume":"107","author":"MS Donnenberg","year":"2001","unstructured":"Donnenberg MS, Whittam TS: Pathogenesis and evolution of virulence in enteropathogenic and enterohemorrhagic Escherichia coli. 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