{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T14:38:20Z","timestamp":1721313500428},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>sabR<\/jats:italic> is a pleiotropic regulatory gene which has been shown to positively regulate the nikkomycin biosynthesis and negatively affect the sporulation of <jats:italic>Streptomyces ansochromogenes<\/jats:italic>. In this study, we investigate the mechanism of SabR on modulating nikkomycin production in <jats:italic>Streptomyces ansochromogenes<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The transcription start point of <jats:italic>sabR<\/jats:italic> was determined by high-resolution S1 nuclease mapping and localized at the nucleotide T at position 37 bp upstream of the potential <jats:italic>sabR<\/jats:italic> translation start codon (GTG). Disruption of <jats:italic>sabR<\/jats:italic> enhanced its own transcription, but retarded the nikkomycin production. Over-expression of <jats:italic>sabR<\/jats:italic> enhanced nikkomycin biosynthesis in <jats:italic>Streptomyces ansochromogenes<\/jats:italic>. EMSA analysis showed that SabR bound to the upstream region of <jats:italic>sanG<\/jats:italic>, but it did not bind to the upstream region of its encoding gene (<jats:italic>sabR<\/jats:italic>), <jats:italic>sanF<\/jats:italic> and the intergenic region between <jats:italic>sanN<\/jats:italic> and <jats:italic>sanO<\/jats:italic>. DNase 1 footprinting assays showed that the SabR-binding site upstream of <jats:italic>sanG<\/jats:italic> was 5'-CTTTAAGTCACCTGGCTCATTCGCGTTCGCCCAGCT-3' which was designated as SARE. Deletion of SARE resulted in the delay of nikkomycin production that was similar to that of <jats:italic>sabR<\/jats:italic> disruption mutant.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>These results indicated that SabR modulated nikkomycin biosynthesis as an enhancer via interaction with the promoter region of <jats:italic>sanG<\/jats:italic>, and expanded our understanding about regulatory cascade in nikkomycin biosynthesis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-11-164","type":"journal-article","created":{"date-parts":[[2011,7,20]],"date-time":"2011-07-20T06:36:30Z","timestamp":1311143790000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes"],"prefix":"10.1186","volume":"11","author":[{"given":"Yuanyuan","family":"Pan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xihong","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqing","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huarong","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,7,20]]},"reference":[{"key":"1441_CR1","doi-asserted-by":"publisher","first-page":"2183","DOI":"10.1099\/00221287-145-9-2183","volume":"145","author":"DA Hopwood","year":"1999","unstructured":"Hopwood DA: Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico. 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