{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:52:37Z","timestamp":1789480357663,"version":"build-2803163510"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>The glutamate dehydrogenase gene (<jats:italic>gdh<\/jats:italic>) is one of the most popular and useful genetic markers for the genotypic analysis of <jats:italic>Giardia duodenalis<\/jats:italic> (syn. <jats:italic>G. lamblia<\/jats:italic>, <jats:italic>G. intestinalis<\/jats:italic>), the protozoan that widely causes enteric disease in humans. To determine the distribution of genotypes of <jats:italic>G. duodenalis<\/jats:italic> in Thai populations and to investigate the extent of sequence variation at this locus, 42 fecal samples were collected from 3 regions of Thailand i.e., Central, Northern, and Eastern regions. All specimens were analyzed using PCR-based genotyping and recombinant subcloning methods.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The results showed that the prevalence of assemblages A and B among these populations was approximately equal, 20 (47.6%) and 22 (52.4%), respectively. Sequence analysis revealed that the nucleotide diversity of assemblage B was significantly greater than that in assemblage A. Among all assemblage B positive specimens, the allelic sequence divergence within isolates was detected. Nine isolates showed mixed alleles, ranged from three to nine distinct alleles per isolate. Statistical analysis demonstrated the occurrence of genetic recombination within subassemblages BIII and BIV was likely.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This study supports increasing evidence that <jats:italic>G. duodenalis<\/jats:italic> has the potential for genetic exchange.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-11-206","type":"journal-article","created":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T18:44:05Z","timestamp":1316544245000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalisfrom Thai Isolates: evidence of genetic exchange or Mixed Infections?"],"prefix":"10.1186","volume":"11","author":[{"given":"Suradej","family":"Siripattanapipong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saovanee","family":"Leelayoova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathirut","family":"Mungthin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RC Andrew","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parima","family":"Boontanom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilai","family":"Saksirisampant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peerapan","family":"Tan-ariya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,9,20]]},"reference":[{"issue":"2","key":"1493_CR1","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1080\/1040-840291046713","volume":"28","author":"S Lane","year":"2002","unstructured":"Lane S, Lloyd D: Current trends in research into the waterborne parasite Giardia. Crit Rev Microbiol. 2002, 28 (2): 123-147. 10.1080\/1040-840291046713.","journal-title":"Crit Rev Microbiol"},{"issue":"5","key":"1493_CR2","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/S0169-4758(99)01624-5","volume":"16","author":"RC Thompson","year":"2000","unstructured":"Thompson RC, Hopkins RM, Homan WL: Nomenclature and genetic groupings of Giardia infecting mammals. Parasitol Today. 2000, 16 (5): 210-213. 10.1016\/S0169-4758(99)01624-5.","journal-title":"Parasitol Today"},{"key":"1493_CR3","volume-title":"World Health Organisation, Geneva","author":"The world health report","year":"1996","unstructured":"The world health report: World Health Organisation. World Health Organisation, Geneva. 1996"},{"issue":"2","key":"1493_CR4","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/0020-7519(89)90006-4","volume":"19","author":"RH Andrews","year":"1989","unstructured":"Andrews RH, Adams M, Boreham PF, Mayrhofer G, Meloni BP: Giardia intestinalis: electrophoretic evidence for a species complex. Int J Parasitol. 1989, 19 (2): 183-190. 10.1016\/0020-7519(89)90006-4.","journal-title":"Int J Parasitol"},{"issue":"9","key":"1493_CR5","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1007\/s004360050474","volume":"84","author":"WL Homan","year":"1998","unstructured":"Homan WL, Gilsing M, Bentala H, Limper L, van Knapen F: Characterization of Giardia duodenalis by polymerase-chain-reaction fingerprinting. Parasitol Res. 1998, 84 (9): 707-714. 10.1007\/s004360050474.","journal-title":"Parasitol Res"},{"issue":"4","key":"1493_CR6","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1007\/BF00937090","volume":"78","author":"WL Homan","year":"1992","unstructured":"Homan WL, van Enckevort FH, Limper L, van Eys GJ, Schoone GJ, Kasprzak W, Majewska AC, van Knapen F: Comparison of Giardia isolates from different laboratories by isoenzyme analysis and recombinant DNA probes. Parasitol Res. 1992, 78 (4): 316-323. 10.1007\/BF00937090.","journal-title":"Parasitol Res"},{"issue":"1","key":"1493_CR7","doi-asserted-by":"publisher","first-page":"44","DOI":"10.2307\/3284315","volume":"83","author":"RM Hopkins","year":"1997","unstructured":"Hopkins RM, Meloni BP, Groth DM, Wetherall JD, Reynoldson JA, Thompson RC: Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality. J Parasitol. 1997, 83 (1): 44-51. 10.2307\/3284315.","journal-title":"J Parasitol"},{"issue":"1","key":"1493_CR8","doi-asserted-by":"crossref","first-page":"65","DOI":"10.4269\/ajtmh.1988.38.65","volume":"38","author":"BP Meloni","year":"1988","unstructured":"Meloni BP, Lymbery AJ, Thompson RC: Isoenzyme electrophoresis of 30 isolates of Giardia from humans and felines. Am J Trop Med Hyg. 1988, 38 (1): 65-73.","journal-title":"Am J Trop Med Hyg"},{"issue":"9","key":"1493_CR9","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.1093\/oxfordjournals.molbev.a026204","volume":"16","author":"PT Monis","year":"1999","unstructured":"Monis PT, Andrews RH, Mayrhofer G, Ey PL: Molecular systematics of the parasitic protozoan Giardia intestinalis. Mol Biol Evol. 1999, 16 (9): 1135-1144.","journal-title":"Mol Biol Evol"},{"issue":"9","key":"1493_CR10","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1016\/j.ijpara.2010.02.015","volume":"40","author":"E Lasek-Nesselquist","year":"2010","unstructured":"Lasek-Nesselquist E, Welch DM, Sogin ML: The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systems. Int J Parasitol. 2010, 40 (9): 1063-1064. 10.1016\/j.ijpara.2010.02.015.","journal-title":"Int J Parasitol"},{"issue":"Pt 3","key":"1493_CR11","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1242\/jcs.95.3.353","volume":"95","author":"KS Kabnick","year":"1990","unstructured":"Kabnick KS, Peattie DA: In situ analyses reveal that the two nuclei of Giardia lamblia are equivalent. J Cell Sci. 1990, 95 (Pt 3): 353-360.","journal-title":"J Cell Sci"},{"issue":"2","key":"1493_CR12","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1128\/EC.1.2.191-199.2002","volume":"1","author":"LZ Yu","year":"2002","unstructured":"Yu LZ, Birky CW, Adam RD: The two nuclei of Giardia each have complete copies of the genome and are partitioned equationally at cytokinesis. Eukaryot Cell. 2002, 1 (2): 191-199. 10.1128\/EC.1.2.191-199.2002.","journal-title":"Eukaryot Cell"},{"issue":"12","key":"1493_CR13","doi-asserted-by":"publisher","first-page":"7866","DOI":"10.1128\/IAI.69.12.7866-7872.2001","volume":"69","author":"S Ghosh","year":"2001","unstructured":"Ghosh S, Frisardi M, Rogers R, Samuelson J: How Giardia swim and divide. Infect Immun. 2001, 69 (12): 7866-7872. 10.1128\/IAI.69.12.7866-7872.2001.","journal-title":"Infect Immun"},{"issue":"1","key":"1493_CR14","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1046\/j.1462-5822.2001.00094.x","volume":"3","author":"R Bernander","year":"2001","unstructured":"Bernander R, Palm JE, Svard SG: Genome ploidy in different stages of the Giardia lamblia life cycle. Cell Microbiol. 2001, 3 (1): 55-62. 10.1046\/j.1462-5822.2001.00094.x.","journal-title":"Cell Microbiol"},{"issue":"Pt 23","key":"1493_CR15","doi-asserted-by":"publisher","first-page":"4889","DOI":"10.1242\/jcs.03276","volume":"119","author":"MS Sagolla","year":"2006","unstructured":"Sagolla MS, Dawson SC, Mancuso JJ, Cande WZ: Three-dimensional analysis of mitosis and cytokinesis in the binucleate parasite Giardia intestinalis. J Cell Sci. 2006, 119 (Pt 23): 4889-4900.","journal-title":"J Cell Sci"},{"issue":"5846","key":"1493_CR16","doi-asserted-by":"publisher","first-page":"1921","DOI":"10.1126\/science.1143837","volume":"317","author":"HG Morrison","year":"2007","unstructured":"Morrison HG, McArthur AG, Gillin FD, Aley SB, Adam RD, Olsen GJ, Best AA, Cande WZ, Chen F, Cipriano MJ, et al: Genomic minimalism in the early diverging intestinal parasite Giardia lamblia. Science. 2007, 317 (5846): 1921-1926. 10.1126\/science.1143837.","journal-title":"Science"},{"issue":"8","key":"1493_CR17","doi-asserted-by":"publisher","first-page":"e1000560","DOI":"10.1371\/journal.ppat.1000560","volume":"5","author":"O Franzen","year":"2009","unstructured":"Franzen O, Jerlstrom-Hultqvist J, Castro E, Sherwood E, Ankarklev J, Reiner DS, Palm D, Andersson JO, Andersson B, Svard SG: Draft genome sequencing of giardia intestinalis assemblage B isolate GS: is human giardiasis caused by two different species?. PLoS Pathog. 2009, 5 (8): e1000560-10.1371\/journal.ppat.1000560.","journal-title":"PLoS Pathog"},{"issue":"8","key":"1493_CR18","doi-asserted-by":"publisher","first-page":"1421","DOI":"10.1128\/EC.00138-07","volume":"6","author":"S Teodorovic","year":"2007","unstructured":"Teodorovic S, Braverman JM, Elmendorf HG: Unusually low levels of genetic variation among Giardia lamblia isolates. Eukaryot Cell. 2007, 6 (8): 1421-1430. 10.1128\/EC.00138-07.","journal-title":"Eukaryot Cell"},{"issue":"22","key":"1493_CR19","doi-asserted-by":"publisher","first-page":"1984","DOI":"10.1016\/j.cub.2007.10.020","volume":"17","author":"MA Cooper","year":"2007","unstructured":"Cooper MA, Adam RD, Worobey M, Sterling CR: Population genetics provides evidence for recombination in Giardia. Curr Biol. 2007, 17 (22): 1984-1988. 10.1016\/j.cub.2007.10.020.","journal-title":"Curr Biol"},{"issue":"2","key":"1493_CR20","first-page":"148","volume":"44","author":"MR O'Grady","year":"1980","unstructured":"O'Grady MR, Slocombe JO: An investigation of variables in a fecal flotation technique. Can J Comp Med. 1980, 44 (2): 148-157.","journal-title":"Can J Comp Med"},{"issue":"2","key":"1493_CR21","first-page":"280","volume":"41","author":"P Boontanom","year":"2010","unstructured":"Boontanom P, Siripattanapipong S, Mungthin M, Tan-ariya P, Leelayoova S: Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen. Southeast Asian J Trop Med Public Health. 2010, 41 (2): 280-284.","journal-title":"Southeast Asian J Trop Med Public Health"},{"issue":"21","key":"1493_CR22","doi-asserted-by":"publisher","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","volume":"23","author":"MA Larkin","year":"2007","unstructured":"Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, et al: Clustal W and Clustal \u00d7 version 2.0. Bioinformatics. 2007, 23 (21): 2947-2948. 10.1093\/bioinformatics\/btm404.","journal-title":"Bioinformatics"},{"issue":"8","key":"1493_CR23","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1093\/bioinformatics\/17.8.754","volume":"17","author":"JP Huelsenbeck","year":"2001","unstructured":"Huelsenbeck JP, Ronquist F: MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001, 17 (8): 754-755. 10.1093\/bioinformatics\/17.8.754.","journal-title":"Bioinformatics"},{"issue":"4","key":"1493_CR24","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1093\/bib\/bbn017","volume":"9","author":"S Kumar","year":"2008","unstructured":"Kumar S, Nei M, Dudley J, Tamura K: MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform. 2008, 9 (4): 299-306. 10.1093\/bib\/bbn017.","journal-title":"Brief Bioinform"},{"issue":"11","key":"1493_CR25","doi-asserted-by":"publisher","first-page":"1451","DOI":"10.1093\/bioinformatics\/btp187","volume":"25","author":"P Librado","year":"2009","unstructured":"Librado P, Rozas J: DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009, 25 (11): 1451-1452. 10.1093\/bioinformatics\/btp187.","journal-title":"Bioinformatics"},{"issue":"13","key":"1493_CR26","doi-asserted-by":"publisher","first-page":"3812","DOI":"10.1093\/nar\/gkg509","volume":"31","author":"PC Ng","year":"2003","unstructured":"Ng PC, Henikoff S: SIFT: Predicting amino acid changes that affect protein function. Nucleic Acids Res. 2003, 31 (13): 3812-3814. 10.1093\/nar\/gkg509.","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"1493_CR27","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1093\/genetics\/123.3.585","volume":"123","author":"F Tajima","year":"1989","unstructured":"Tajima F: Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics. 1989, 123 (3): 585-595.","journal-title":"Genetics"},{"key":"1493_CR28","volume-title":"Evolutionary genetics","author":"MS John","year":"1989","unstructured":"John MS: Evolutionary genetics. 1989, Oxford: Oxford University Press"},{"issue":"2","key":"1493_CR29","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1093\/molbev\/msj030","volume":"23","author":"DH Huson","year":"2006","unstructured":"Huson DH, Bryant D: Application of phylogenetic networks in evolutionary studies. Mol Biol Evol. 2006, 23 (2): 254-267.","journal-title":"Mol Biol Evol"},{"issue":"2","key":"1493_CR30","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1093\/molbev\/msh018","volume":"21","author":"D Bryant","year":"2004","unstructured":"Bryant D, Moulton V: Neighbor-net: an agglomerative method for the construction of phylogenetic networks. Mol Biol Evol. 2004, 21 (2): 255-265.","journal-title":"Mol Biol Evol"},{"issue":"4","key":"1493_CR31","doi-asserted-by":"publisher","first-page":"2665","DOI":"10.1534\/genetics.105.048975","volume":"172","author":"TC Bruen","year":"2006","unstructured":"Bruen TC, Philippe H, Bryant D: A simple and robust statistical test for detecting the presence of recombination. Genetics. 2006, 172 (4): 2665-2681.","journal-title":"Genetics"},{"issue":"2","key":"1493_CR32","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/0040-5809(83)90013-8","volume":"23","author":"RR Hudson","year":"1983","unstructured":"Hudson RR: Properties of a neutral allele model with intragenic recombination. Theor Popul Biol. 1983, 23 (2): 183-201. 10.1016\/0040-5809(83)90013-8.","journal-title":"Theor Popul Biol"},{"issue":"2","key":"1493_CR33","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.molbiopara.2008.04.006","volume":"160","author":"SM Caccio","year":"2008","unstructured":"Caccio SM, Ryan U: Molecular epidemiology of giardiasis. Mol Biochem Parasitol. 2008, 160 (2): 75-80. 10.1016\/j.molbiopara.2008.04.006.","journal-title":"Mol Biochem Parasitol"},{"issue":"12","key":"1493_CR34","doi-asserted-by":"publisher","first-page":"e558","DOI":"10.1371\/journal.pntd.0000558","volume":"3","author":"H Sprong","year":"2009","unstructured":"Sprong H, Caccio SM, van der Giessen JW: Identification of zoonotic genotypes of Giardia duodenalis. PLoS Negl Trop Dis. 2009, 3 (12): e558-10.1371\/journal.pntd.0000558.","journal-title":"PLoS Negl Trop Dis"},{"key":"1493_CR35","doi-asserted-by":"publisher","first-page":"(1","DOI":"10.1016\/j.gene.2009.09.010","volume":"449","author":"R Kosuwin","year":"2010","unstructured":"Kosuwin R, Putaporntip C, Pattanawong U, Jongwutiwes S: Clonal diversity in Giardia duodenalis isolates from Thailand: evidences for intragenic recombination and purifying selection at the beta giardin locus. Gene. 2010, 449: (1-2):1-8. 10.1016\/j.gene.2009.09.010.","journal-title":"Gene"},{"issue":"24","key":"1493_CR36","doi-asserted-by":"publisher","first-page":"10500","DOI":"10.1093\/nar\/17.24.10500","volume":"17","author":"JM Cock","year":"1989","unstructured":"Cock JM, Schmidt RR: A glutamate dehydrogenase gene sequence. Nucleic Acids Res. 1989, 17 (24): 10500-10.1093\/nar\/17.24.10500.","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"1493_CR37","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1017\/S0031182009990436","volume":"136","author":"T Geurden","year":"2009","unstructured":"Geurden T, Levecke B, Caccio SM, Visser A, De Groote G, Casaert S, Vercruysse J, Claerebout E: Multilocus genotyping of Cryptosporidium and Giardia in non-outbreak related cases of diarrhoea in human patients in Belgium. Parasitology. 2009, 136 (10): 1161-1168. 10.1017\/S0031182009990436.","journal-title":"Parasitology"},{"issue":"2","key":"1493_CR38","first-page":"185","volume":"15","author":"MA Ramesh","year":"2005","unstructured":"Ramesh MA, Malik SB, Logsdon JM: A phylogenomic inventory of meiotic genes; evidence for sex in Giardia and an early eukaryotic origin of meiosis. Curr Biol. 2005, 15 (2): 185-191.","journal-title":"Curr Biol"},{"issue":"6","key":"1493_CR39","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1111\/j.1550-7408.2009.00443.x","volume":"56","author":"E Lasek-Nesselquist","year":"2009","unstructured":"Lasek-Nesselquist E, Welch DM, Thompson RC, Steuart RF, Sogin ML: Genetic exchange within and between assemblages of Giardia duodenalis. J Eukaryot Microbiol. 2009, 56 (6): 504-518. 10.1111\/j.1550-7408.2009.00443.x.","journal-title":"J Eukaryot Microbiol"},{"issue":"5","key":"1493_CR40","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1093\/oxfordjournals.molbev.a004129","volume":"19","author":"D Posada","year":"2002","unstructured":"Posada D: Evaluation of methods for detecting recombination from DNA sequences: empirical data. Mol Biol Evol. 2002, 19 (5): 708-717.","journal-title":"Mol Biol Evol"},{"key":"1493_CR41","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1017\/CBO9780511819049.017","volume-title":"The Phylogenetic Handbook: A Practical Approach to Phylogenetic Analysis and Hypothesis Testing","author":"P Lemey","year":"2009","unstructured":"Lemey P, Posada D: Introduction to recombination detection. The Phylogenetic Handbook: A Practical Approach to Phylogenetic Analysis and Hypothesis Testing. Edited by: Lemey P, Salemi M, and Vandamme AM. 2009, New York: Cambridge University Press, 493-518. 2nd","edition":"2nd"},{"issue":"7","key":"1493_CR42","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1093\/molbev\/msn083","volume":"25","author":"D Posada","year":"2008","unstructured":"Posada D: jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008, 25 (7): 1253-1256. 10.1093\/molbev\/msn083.","journal-title":"Mol Biol Evol"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2180-11-206.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T16:51:00Z","timestamp":1630515060000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/1471-2180-11-206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,20]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["1493"],"URL":"https:\/\/doi.org\/10.1186\/1471-2180-11-206","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,20]]},"assertion":[{"value":"23 March 2011","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 September 2011","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 September 2011","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"206"}}