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Matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a rapid method for the analysis of biological samples. The advantages of this method, compared to conventional techniques, are rapidity, cost-effectiveness, accuracy and suitability for the high-throughput identification of bacteria. Discrepancies between taxonomy and genetic relatedness on the species and biovar level complicate the development of detection and identification assays.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In this study, the accurate identification of <jats:italic>Brucella<\/jats:italic> species using MALDI-TOF-MS was achieved by constructing a <jats:italic>Brucella<\/jats:italic> reference library based on multilocus variable-number tandem repeat analysis (MLVA) data. By comparing MS-spectra from <jats:italic>Brucella<\/jats:italic> species against a custom-made MALDI-TOF-MS reference library, MALDI-TOF-MS could be used as a rapid identification method for <jats:italic>Brucella<\/jats:italic> species. In this way, 99.3% of the 152 isolates tested were identified at the species level, and <jats:italic>B. suis<\/jats:italic> biovar 1 and 2 were identified at the level of their biovar. This result demonstrates that for <jats:italic>Brucella<\/jats:italic>, even minimal genomic differences between these serovars translate to specific proteomic differences.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>MALDI-TOF-MS can be developed into a fast and reliable identification method for genetically highly related species when potential taxonomic and genetic inconsistencies are taken into consideration during the generation of the reference library.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-11-267","type":"journal-article","created":{"date-parts":[[2012,3,28]],"date-time":"2012-03-28T14:15:13Z","timestamp":1332944113000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS"],"prefix":"10.1186","volume":"11","author":[{"given":"Florigio","family":"Lista","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans AG","family":"Reubsaet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"De Santis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene R","family":"Parchen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ad L","family":"de Jong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasper","family":"Kieboom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton L","family":"van der Laaken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid AI","family":"Voskamp-Visser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Fillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo-Jan","family":"Jansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Van der Plas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armand","family":"Paauw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,12,23]]},"reference":[{"key":"1598_CR1","first-page":"370","volume-title":"Volume 2 part C","author":"DJ Brenner","year":"2005","unstructured":"Brenner DJ, Krieg NR, Staley JT, Corbel MJ, Banai M: Bergey's Manual of Systemic Bacteriology. 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