{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T09:12:39Z","timestamp":1772788359326,"version":"3.50.1"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Burkholderia pseudomallei<\/jats:italic> is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes in <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic>. Complicating this strategy are the four different <jats:italic>B. pseudomallei<\/jats:italic> LPS O-antigen types: A, B, B2, and rough. Sero-crossreactivity is common among O-antigens of <jats:italic>Burkholderia<\/jats:italic> species. Here, we identified the presence of multiple <jats:italic>B. pseudomallei<\/jats:italic> O-antigen types and sero-crossreactivity in its near-neighbor species.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>PCR screening of O-antigen biosynthesis genes, phenotypic characterization using SDS-PAGE, and immunoblot analysis showed that majority of <jats:italic>B. mallei<\/jats:italic> and <jats:italic>B. thailandensis<\/jats:italic> strains contained the typical O-antigen type A. In contrast, most of <jats:italic>B. ubonensis<\/jats:italic> and <jats:italic>B. thailandensis<\/jats:italic>-like strains expressed the atypical O-antigen types B and B2, respectively. Most <jats:italic>B<\/jats:italic>. <jats:italic>oklahomensis<\/jats:italic> strains expressed a distinct and non-seroreactive O-antigen type, except strain E0147 which expressed O-antigen type A. O-antigen type B2 was also detected in <jats:italic>B<\/jats:italic>. <jats:italic>thailandensis<\/jats:italic> 82172, <jats:italic>B<\/jats:italic>. <jats:italic>ubonensis<\/jats:italic> MSMB108, and <jats:italic>Burkholderia<\/jats:italic> sp. MSMB175. Interestingly, <jats:italic>B<\/jats:italic>. <jats:italic>thailandensis<\/jats:italic>-like MSMB43 contained a novel serotype B positive O-antigen.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This study expands the number of species which express <jats:italic>B. pseudomallei<\/jats:italic> O-antigen types. Further work is required to elucidate the full structures and how closely these are to the <jats:italic>B. pseudomallei<\/jats:italic> O-antigens, which will ultimately determine the efficacy of the near-neighbor B serotypes for vaccine development.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-12-250","type":"journal-article","created":{"date-parts":[[2012,11,5]],"date-time":"2012-11-05T15:14:12Z","timestamp":1352128452000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species"],"prefix":"10.1186","volume":"12","author":[{"given":"Joshua K","family":"Stone","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Mayo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie A","family":"Grasso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L","family":"Ginther","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie D","family":"Warrington","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J","family":"Allender","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adina","family":"Doyle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shalamar","family":"Georgia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirjam","family":"Kaestli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacey M","family":"Broomall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A","family":"Karavis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph M","family":"Insalaco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle S","family":"Hubbard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren A","family":"McNew","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry S","family":"Gibbons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart J","family":"Currie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Keim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Apichai","family":"Tuanyok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,11,5]]},"reference":[{"key":"1818_CR1","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1146\/annurev.biochem.71.110601.135414","volume":"71","author":"CRH Raetz","year":"2002","unstructured":"Raetz CRH, Whitfield C: Lipopolysaccharide endotoxins. 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