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However, contradictionary results on subcellular localization of PHB granules in <jats:italic>Ralstonia eutropha<\/jats:italic> were published, recently.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Here, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in <jats:italic>R. eutropha<\/jats:italic> during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of <jats:italic>\u2206phaM<\/jats:italic> strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Subcellular localization of PHB granules is controlled in <jats:italic>R. eutropha<\/jats:italic> and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-12-262","type":"journal-article","created":{"date-parts":[[2012,11,17]],"date-time":"2012-11-17T01:14:37Z","timestamp":1353114877000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha"],"prefix":"10.1186","volume":"12","author":[{"given":"Andreas","family":"Wahl","sequence":"first","affiliation":[]},{"given":"Nora","family":"Schuth","sequence":"additional","affiliation":[]},{"given":"Daniel","family":"Pfeiffer","sequence":"additional","affiliation":[]},{"given":"Stephan","family":"Nussberger","sequence":"additional","affiliation":[]},{"given":"Dieter","family":"Jendrossek","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2012,11,16]]},"reference":[{"key":"1853_CR1","doi-asserted-by":"publisher","first-page":"5132","DOI":"10.1002\/pmic.200900333","volume":"9","author":"E Schwartz","year":"2009","unstructured":"Schwartz E, Voigt B, Z\u00fchlke D, Pohlmann A, Lenz O, Albrecht D, Schwarze A, Kohlmann Y, Krause C, Hecker M, Friedrich B: A proteomic view of the facultatively chemolithoautotrophic lifestyle of Ralstonia eutropha H16. 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