{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:58:17Z","timestamp":1773658697505,"version":"3.50.1"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Burkholderia pseudomallei<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> are closely related Category B Select Agents of bioterrorism and the causative agents of the diseases melioidosis and glanders, respectively. Rapid phage-based diagnostic tools would greatly benefit early recognition and treatment of these diseases. There is extensive strain-to-strain variation in <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> genome content due in part to the presence or absence of integrated prophages. Several phages have previously been isolated from <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> lysogens, for example \u03c6K96243, \u03c61026b and \u03c652237.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We have isolated a P2-like bacteriophage, \u03c6X216, which infects 78% of all <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> strains tested. \u03c6X216 also infects <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic>, but not other <jats:italic>Burkholderia<\/jats:italic> species, including the closely related <jats:italic>B<\/jats:italic>. <jats:italic>thailandensis<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>oklahomensis<\/jats:italic>. The nature of the \u03c6X216 host receptor remains unclear but evidence indicates that in <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> \u03c6X216 uses lipopolysaccharide O-antigen but a different receptor in <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic>. The 37,637 bp genome of \u03c6X216 encodes 47 predicted open reading frames and shares 99.8% pairwise identity and an identical strain host range with bacteriophage \u03c652237. Closely related P2-like prophages appear to be widely distributed among <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> strains but both \u03c6X216 and \u03c652237 readily infect prophage carrying strains.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The broad strain infectivity and high specificity for <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> indicate that \u03c6X216 will provide a good platform for the development of phage-based diagnostics for these bacteria.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-12-289","type":"journal-article","created":{"date-parts":[[2012,12,7]],"date-time":"2012-12-07T13:14:30Z","timestamp":1354886070000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["\u03c6X216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. malleistrain infectivity"],"prefix":"10.1186","volume":"12","author":[{"given":"Brian H","family":"Kvitko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher R","family":"Cox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"DeShazer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon L","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kent J","family":"Voorhees","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert P","family":"Schweizer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,12,7]]},"reference":[{"issue":"2","key":"1838_CR1","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","volume":"18","author":"AC Cheng","year":"2005","unstructured":"Cheng AC, Currie BJ: Melioidosis: epidemiology, pathophysiology, and management. 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