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In this study, we surveyed the sequence variance of <jats:italic>rfbT<\/jats:italic> in the Ogawa and Inaba strains in China over a 48-year (1961-2008) period in which serotype shifts occurred among epidemic years.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Various mutation events including single nucleotide, short fragment insertions\/deletions and transposases insertions, were found in the <jats:italic>rfbT<\/jats:italic> gene of the Inaba strains. Ectopically introducing an intact <jats:italic>rfbT<\/jats:italic> could overcome the mutations by converting the Inaba serotype to the Ogawa serotype, suggesting the effects of these mutations on the function of RfbT. Characteristic <jats:italic>rfbT<\/jats:italic> mutations were recognized in the Inaba strains among Inaba serotype dominant epidemic years which were separate from the Ogawa dominant epidemics. Three distinguishable mutation sites in <jats:italic>rfbT<\/jats:italic> between the classical and the El Tor biotype strains were identified and could serve as biotype-specific biomarkers.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Our results provide a comprehensive picture of the <jats:italic>rfbT<\/jats:italic> gene mutations among the <jats:italic>V. cholerae<\/jats:italic> O1 strains in different epidemic periods, which could be further used as the tracing markers in clonality analysis and dissemination surveillance of the epidemic strains.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-13-173","type":"journal-article","created":{"date-parts":[[2013,7,26]],"date-time":"2013-07-26T15:13:11Z","timestamp":1374851591000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Sequence polymorphisms of rfbT among the Vibrio cholerae O1 strains in the Ogawa and Inaba serotype shifts"],"prefix":"10.1186","volume":"13","author":[{"given":"Weili","family":"Liang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luxi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pu","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijian","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Kan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,7,26]]},"reference":[{"issue":"4","key":"2047_CR1","doi-asserted-by":"publisher","first-page":"1487","DOI":"10.1084\/jem.168.4.1487","volume":"168","author":"DA Herrington","year":"1988","unstructured":"Herrington DA, Hall RH, Losonsky G, Mekalanos JJ, Taylor RK, Levine MM: Toxin, toxin-coregulated pili, and the toxR regulon are essential for Vibrio cholerae pathogenesis in humans. 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