{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:45:17Z","timestamp":1790873117133,"version":"4.1.0"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Brucella<\/jats:italic> is an intracellular pathogen capable of infecting animals and humans. There are six recognized species of <jats:italic>Brucella<\/jats:italic> that differ in their host preference. The genomes of the three <jats:italic>Brucella<\/jats:italic> species have been recently sequenced. Comparison of the three revealed over 98% sequence similarity at the protein level and enabled computational identification of common and differentiating genes. We validated these computational predictions and examined the expression patterns of the putative unique and differentiating genes, using genomic and reverse transcription PCR. We then screened a set of differentiating genes against classical <jats:italic>Brucella<\/jats:italic> biovars and showed the applicability of these regions in the design of diagnostic tests.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We have identified and tested set of molecular targets that are associated in unique patterns with each of the sequenced <jats:italic>Brucella<\/jats:italic> spp. A comprehensive comparison was made among the published genome sequences of <jats:italic>B. abortus, B. melitensis<\/jats:italic> and <jats:italic>B. suis<\/jats:italic>. The comparison confirmed published differences between the three <jats:italic>Brucella<\/jats:italic> genomes, and identified subsets of features that were predicted to be of interest in a functional comparison of <jats:italic>B. melitensis<\/jats:italic> and <jats:italic>B. suis<\/jats:italic> to <jats:italic>B. abortus<\/jats:italic>. Differentiating sequence regions from <jats:italic>B. abortus, B. melitensis<\/jats:italic> and <jats:italic>B. suis<\/jats:italic> were used to develop PCR primers to test for the existence and <jats:italic>in vitro<\/jats:italic> transcription of these genes in these species. Only <jats:italic>B. suis<\/jats:italic> is found to have a significant number of unique genes, but combinations of genes and regions that exist in only two out of three genomes and are therefore useful for diagnostics were identified and confirmed.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Although not all of the differentiating genes identified were transcribed under steady state conditions, a group of genes sufficient to discriminate unambiguously between <jats:italic>B. suis<\/jats:italic>, <jats:italic>B. melitensis<\/jats:italic>, and <jats:italic>B. abortus<\/jats:italic> was identified. We present an overview of these genomic differences and the use of these features to discriminate among a number of <jats:italic>Brucella<\/jats:italic> biovars.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-6-13","type":"journal-article","created":{"date-parts":[[2006,2,24]],"date-time":"2006-02-24T07:32:14Z","timestamp":1140766334000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Molecular targets for rapid identification of Brucella spp"],"prefix":"10.1186","volume":"6","author":[{"given":"Vladyslava G","family":"Ratushna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M","family":"Sturgill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheela","family":"Ramamoorthy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherry A","family":"Reichow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqun","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raju","family":"Lathigra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nammalwar","family":"Sriranganathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirley M","family":"Halling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen M","family":"Boyle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia J","family":"Gibas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2006,2,22]]},"reference":[{"issue":"1","key":"226_CR1","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/S1369-5274(00)00165-X","volume":"4","author":"ML Boschiroli","year":"2001","unstructured":"Boschiroli ML, Foulongne V, O'Callaghan D: Brucellosis: a worldwide zoonosis. 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