{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:35:44Z","timestamp":1789583744058,"version":"build-2803163510"},"reference-count":68,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Sporothrix schenckii<\/jats:italic> is a pathogenic, dimorphic fungus, the etiological agent of sporotrichosis, a subcutaneous lymphatic mycosis. Dimorphism in <jats:italic>S. schenckii<\/jats:italic> responds to second messengers such as cAMP and calcium, suggesting the possible involvement of a calcium\/calmodulin kinase in its regulation. In this study we describe a novel calcium\/calmodulin-dependent protein kinase gene in <jats:italic>S. schenckii, sscmk1<\/jats:italic>, and the effects of inhibitors of calmodulin and calcium\/calmodulin kinases on the yeast to mycelium transition and the yeast cell cycle.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Using the PCR homology approach a new member of the calcium\/calmodulin kinase family, SSCMK1, was identified in this fungus. The cDNA sequence of <jats:italic>sscmk1<\/jats:italic> revealed an open reading frame of 1,221 nucleotides encoding a 407 amino acid protein with a predicted molecular weight of 45.6 kDa. The genomic sequence of <jats:italic>sscmk1<\/jats:italic> revealed the same ORF interrupted by five introns. Bioinformatic analyses of SSCMK1 showed that this protein had the distinctive features that characterize a calcium\/calmodulin protein kinase: a serine\/threonine protein kinase domain and a calmodulin-binding domain. When compared to homologues from seven species of filamentous fungi, SSCMK1 showed substantial similarities, except for a large and highly variable region that encompasses positions 330 \u2013 380 of the multiple sequence alignment. Inhibition studies using calmodulin inhibitor W-7, and calcium\/calmodulin kinase inhibitors, KN-62 and lavendustin C, were found to inhibit budding by cells induced to re-enter the yeast cell cycle and to favor the yeast to mycelium transition.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This study constitutes the first evidence of the presence of a calcium\/calmodulin kinase-encoding gene in <jats:italic>S. schenckii<\/jats:italic> and its possible involvement as an effector of dimorphism in this fungus. These results suggest that a calcium\/calmodulin dependent signaling pathway could be involved in the regulation of dimorphism in this fungus. The results suggest that the calcium\/calmodulin kinases of yeasts are evolutionarily distinct from those in filamentous fungi.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-7-107","type":"journal-article","created":{"date-parts":[[2007,11,29]],"date-time":"2007-11-29T19:14:09Z","timestamp":1196363649000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Functional, genetic and bioinformatic characterization of a calcium\/calmodulin kinase gene in Sporothrix schenckii"],"prefix":"10.1186","volume":"7","author":[{"given":"Liz","family":"Valle-Aviles","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirley","family":"Valentin-Berrios","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo R","family":"Gonzalez-Mendez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuri","family":"Rodriguez-del Valle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,11,29]]},"reference":[{"issue":"4","key":"424_CR1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1128\/mr.44.4.683-721.1980","volume":"44","author":"LR Travassos","year":"1980","unstructured":"Travassos LR, Lloyd KO: Sporothrix schenckii and related species of Ceratocystis. Microbiol Rev. 1980, 44 (4): 683-721.","journal-title":"Microbiol Rev"},{"key":"424_CR2","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1007\/978-1-4615-2834-0_19","volume-title":"Dimorphic Fungi in Biology and Medicine","author":"W Colon-Colon","year":"1993","unstructured":"Colon-Colon W, Rodriguez-Del Valle N: Studies on phase transitions in Sporothrix schenckii; Possible involvement of protein kinase C. Dimorphic Fungi in Biology and Medicine. Edited by: H VB, FC O, D K. 1993, New York , Plenum Publishing Company, 225-246."},{"issue":"1","key":"424_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BF01795170","volume":"112","author":"S Serrano","year":"1990","unstructured":"Serrano S, Rodriguez-del Valle N: Calcium uptake and efflux during the yeast to mycelium transition in Sporothrix schenckii. Mycopathologia. 1990, 112 (1): 1-9. 10.1007\/BF01795170.","journal-title":"Mycopathologia"},{"issue":"1","key":"424_CR4","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1006\/scdb.2000.0211","volume":"12","author":"MD Bootman","year":"2001","unstructured":"Bootman MD, Collins TJ, Peppiatt CM, Prothero LS, MacKenzie L, De Smet P, Travers M, Tovey SC, Seo JT, Berridge MJ, Ciccolini F, Lipp P: Calcium signalling--an overview. Semin Cell Dev Biol. 2001, 12 (1): 3-10. 10.1006\/scdb.2000.0211.","journal-title":"Semin Cell Dev Biol"},{"issue":"Pt 12","key":"424_CR5","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1242\/jcs.114.12.2213","volume":"114","author":"MD Bootman","year":"2001","unstructured":"Bootman MD, Lipp P, Berridge MJ: The organisation and functions of local Ca(2+) signals. J Cell Sci. 2001, 114 (Pt 12): 2213-2222.","journal-title":"J Cell Sci"},{"issue":"7","key":"424_CR6","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1038\/nrm1155","volume":"4","author":"MJ Berridge","year":"2003","unstructured":"Berridge MJ, Bootman MD, Roderick HL: Calcium signalling: dynamics, homeostasis and remodelling. Nat Rev Mol Cell Biol. 2003, 4 (7): 517-529. 10.1038\/nrm1155.","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"1-3","key":"424_CR7","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.bbamcr.2004.08.012","volume":"1742","author":"MJ Berridge","year":"2004","unstructured":"Berridge MJ: Calcium signal transduction and cellular control mechanisms. Biochim Biophys Acta. 2004, 1742 (1-3): 3-7. 10.1016\/j.bbamcr.2004.08.012.","journal-title":"Biochim Biophys Acta"},{"key":"424_CR8","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1002\/0470846674.ch6","volume":"239","author":"MJ Berridge","year":"2001","unstructured":"Berridge MJ: The versatility and complexity of calcium signalling. Novartis Found Symp. 2001, 239: 52-64; discussion 64-7, 150-9.","journal-title":"Novartis Found Symp"},{"issue":"8","key":"424_CR9","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/S0962-8924(00)01800-6","volume":"10","author":"D Chin","year":"2000","unstructured":"Chin D, Means AR: Calmodulin: a prototypical calcium sensor. Trends Cell Biol. 2000, 10 (8): 322-328. 10.1016\/S0962-8924(00)01800-6.","journal-title":"Trends Cell Biol"},{"key":"424_CR10","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1146\/annurev.pharmtox.41.1.471","volume":"41","author":"SS Hook","year":"2001","unstructured":"Hook SS, Means AR: Ca(2+)\/CaM-dependent kinases: from activation to function. Annu Rev Pharmacol Toxicol. 2001, 41: 471-505. 10.1146\/annurev.pharmtox.41.1.471.","journal-title":"Annu Rev Pharmacol Toxicol"},{"issue":"4","key":"424_CR11","first-page":"295","volume":"5","author":"AC Nairn","year":"1994","unstructured":"Nairn AC, Picciotto MR: Calcium\/calmodulin-dependent protein kinases. Semin Cancer Biol. 1994, 5 (4): 295-303.","journal-title":"Semin Cancer Biol"},{"issue":"Pt 3","key":"424_CR12","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1042\/bj20020228","volume":"364","author":"A Hudmon","year":"2002","unstructured":"Hudmon A, Schulman H: Structure-function of the multifunctional Ca2+\/calmodulin-dependent protein kinase II. Biochem J. 2002, 364 (Pt 3): 593-611. 10.1042\/BJ20020228.","journal-title":"Biochem J"},{"issue":"1","key":"424_CR13","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1210\/mend.14.1.0414","volume":"14","author":"AR Means","year":"2000","unstructured":"Means AR: Regulatory cascades involving calmodulin-dependent protein kinases. Mol Endocrinol. 2000, 14 (1): 4-13. 10.1210\/me.14.1.4.","journal-title":"Mol Endocrinol"},{"issue":"5760","key":"424_CR14","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1038\/285073a0","volume":"285","author":"AR Means","year":"1980","unstructured":"Means AR, Dedman JR: Calmodulin--an intracellular calcium receptor. Nature. 1980, 285 (5760): 73-77. 10.1038\/285073a0.","journal-title":"Nature"},{"key":"424_CR15","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1146\/annurev.ph.57.030195.002221","volume":"57","author":"AP Braun","year":"1995","unstructured":"Braun AP, Schulman H: The multifunctional calcium\/calmodulin-dependent protein kinase: from form to function. Annu Rev Physiol. 1995, 57: 417-445. 10.1146\/annurev.ph.57.030195.002221.","journal-title":"Annu Rev Physiol"},{"issue":"4","key":"424_CR16","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/oxfordjournals.jbchem.a124617","volume":"116","author":"S Okuno","year":"1994","unstructured":"Okuno S, Kitani T, Fujisawa H: Purification and characterization of Ca2+\/calmodulin-dependent protein kinase IV kinase from rat brain. J Biochem (Tokyo). 1994, 116 (4): 923-930.","journal-title":"J Biochem (Tokyo)"},{"issue":"35","key":"424_CR17","doi-asserted-by":"crossref","first-page":"26512","DOI":"10.1016\/S0021-9258(19)74343-9","volume":"268","author":"MR Picciotto","year":"1993","unstructured":"Picciotto MR, Czernik AJ, Nairn AC: Calcium\/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site. J Biol Chem. 1993, 268 (35): 26512-26521.","journal-title":"J Biol Chem"},{"issue":"27","key":"424_CR18","doi-asserted-by":"crossref","first-page":"14471","DOI":"10.1016\/S0021-9258(17)38593-9","volume":"260","author":"RB Pearson","year":"1985","unstructured":"Pearson RB, Woodgett JR, Cohen P, Kemp BE: Substrate specificity of a multifunctional calmodulin-dependent protein kinase. J Biol Chem. 1985, 260 (27): 14471-14476.","journal-title":"J Biol Chem"},{"issue":"6","key":"424_CR19","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1002\/j.1460-2075.1991.tb07671.x","volume":"10","author":"MH Pausch","year":"1991","unstructured":"Pausch MH, Kaim D, Kunisawa R, Admon A, Thorner J: Multiple Ca2+\/calmodulin-dependent protein kinase genes in a unicellular eukaryote. Embo J. 1991, 10 (6): 1511-1522.","journal-title":"Embo J"},{"issue":"9","key":"424_CR20","doi-asserted-by":"publisher","first-page":"4824","DOI":"10.1128\/MCB.16.9.4824","volume":"16","author":"MJ Moser","year":"1996","unstructured":"Moser MJ, Geiser JR, Davis TN: Ca2+-calmodulin promotes survival of pheromone-induced growth arrest by activation of calcineurin and Ca2+-calmodulin-dependent protein kinase. Mol Cell Biol. 1996, 16 (9): 4824-4831.","journal-title":"Mol Cell Biol"},{"issue":"1","key":"424_CR21","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1074\/jbc.275.1.685","volume":"275","author":"CD Rasmussen","year":"2000","unstructured":"Rasmussen CD: Cloning of a calmodulin kinase I homologue from Schizosaccharomyces pombe. J Biol Chem. 2000, 275 (1): 685-690. 10.1074\/jbc.275.1.685.","journal-title":"J Biol Chem"},{"issue":"49","key":"424_CR22","doi-asserted-by":"publisher","first-page":"38230","DOI":"10.1074\/jbc.M006422200","volume":"275","author":"JD Joseph","year":"2000","unstructured":"Joseph JD, Means AR: Identification and characterization of two Ca2+\/CaM-dependent protein kinases required for normal nuclear division in Aspergillus nidulans. J Biol Chem. 2000, 275 (49): 38230-38238. 10.1074\/jbc.M006422200.","journal-title":"J Biol Chem"},{"issue":"1","key":"424_CR23","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1128\/EC.01.1.119-125.2002","volume":"1","author":"JD Joseph","year":"2002","unstructured":"Joseph JD, Means AR: Calcium binding is required for calmodulin function in Aspergillus nidulans. Eukaryot Cell. 2002, 1 (1): 119-125. 10.1128\/EC.01.1.119-125.2002.","journal-title":"Eukaryot Cell"},{"issue":"1","key":"424_CR24","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/0378-1119(92)90671-B","volume":"113","author":"LB Kornstein","year":"1992","unstructured":"Kornstein LB, Gaiso ML, Hammell RL, Bartelt DC: Cloning and sequence determination of a cDNA encoding Aspergillus nidulans calmodulin-dependent multifunctional protein kinase. Gene. 1992, 113 (1): 75-82. 10.1016\/0378-1119(92)90671-B.","journal-title":"Gene"},{"issue":"23","key":"424_CR25","doi-asserted-by":"crossref","first-page":"13767","DOI":"10.1016\/S0021-9258(18)77415-2","volume":"265","author":"CD Rasmussen","year":"1990","unstructured":"Rasmussen CD, Means RL, Lu KP, May GS, Means AR: Characterization and expression of the unique calmodulin gene of Aspergillus nidulans. J Biol Chem. 1990, 265 (23): 13767-13775.","journal-title":"J Biol Chem"},{"issue":"44","key":"424_CR26","doi-asserted-by":"publisher","first-page":"41064","DOI":"10.1074\/jbc.M106905200","volume":"276","author":"Y Yang","year":"2001","unstructured":"Yang Y, Cheng P, Zhi G, Liu Y: Identification of a calcium\/calmodulin-dependent protein kinase that phosphorylates the Neurospora circadian clock protein FREQUENCY. J Biol Chem. 2001, 276 (44): 41064-41072. 10.1074\/jbc.M106905200.","journal-title":"J Biol Chem"},{"issue":"1","key":"424_CR27","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1111\/j.1574-6968.2002.tb11237.x","volume":"212","author":"PJ Tsai","year":"2002","unstructured":"Tsai PJ, Tu J, Chen TH: Cloning of a Ca(2+)\/calmodulin-dependent protein kinase gene from the filamentous fungus Arthrobotrys dactyloides. FEMS Microbiol Lett. 2002, 212 (1): 7-13. 10.1111\/j.1574-6968.2002.tb11237.x.","journal-title":"FEMS Microbiol Lett"},{"issue":"23","key":"424_CR28","doi-asserted-by":"publisher","first-page":"4878","DOI":"10.1093\/nar\/23.23.4878","volume":"23","author":"K Quandt","year":"1995","unstructured":"Quandt K, Frech K, Karas H, Wingender E, Werner T: MatInd and MatInspector: new fast and versatile tools for detection of consensus matches in nucleotide sequence data. Nucleic Acids Res. 1995, 23 (23): 4878-4884. 10.1093\/nar\/23.23.4878.","journal-title":"Nucleic Acids Res"},{"issue":"5","key":"424_CR29","doi-asserted-by":"publisher","first-page":"2808","DOI":"10.1073\/pnas.051634198","volume":"98","author":"GY Wu","year":"2001","unstructured":"Wu GY, Deisseroth K, Tsien RW: Activity-dependent CREB phosphorylation: convergence of a fast, sensitive calmodulin kinase pathway and a slow, less sensitive mitogen-activated protein kinase pathway. Proc Natl Acad Sci U S A. 2001, 98 (5): 2808-2813. 10.1073\/pnas.051634198.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"Web Server issu","key":"424_CR30","doi-asserted-by":"publisher","first-page":"W362","DOI":"10.1093\/nar\/gkl124","volume":"34","author":"E de Castro","year":"2006","unstructured":"de Castro E, Sigrist CJ, Gattiker A, Bulliard V, Langendijk-Genevaux PS, Gasteiger E, Bairoch A, Hulo N: ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins. Nucleic Acids Res. 2006, 34 (Web Server issue): W362-5. 10.1093\/nar\/gkl124.","journal-title":"Nucleic Acids Res"},{"issue":"Database issue","key":"424_CR31","doi-asserted-by":"publisher","first-page":"D247","DOI":"10.1093\/nar\/gkj149","volume":"34","author":"RD Finn","year":"2006","unstructured":"Finn RD, Mistry J, Schuster-Bockler B, Griffiths-Jones S, Hollich V, Lassmann T, Moxon S, Marshall M, Khanna A, Durbin R, Eddy SR, Sonnhammer EL, Bateman A: Pfam: clans, web tools and services. Nucleic Acids Res. 2006, 34 (Database issue): D247-51. 10.1093\/nar\/gkj149.","journal-title":"Nucleic Acids Res"},{"issue":"8","key":"424_CR32","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1096\/fasebj.9.8.7768349","volume":"9","author":"SK Hanks","year":"1995","unstructured":"Hanks SK, Hunter T: Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. Faseb J. 1995, 9 (8): 576-596.","journal-title":"Faseb J"},{"issue":"4861","key":"424_CR33","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1126\/science.3291115","volume":"241","author":"SK Hanks","year":"1988","unstructured":"Hanks SK, Quinn AM, Hunter T: The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science. 1988, 241 (4861): 42-52. 10.1126\/science.3291115.","journal-title":"Science"},{"issue":"9","key":"424_CR34","doi-asserted-by":"publisher","first-page":"2129","DOI":"10.1101\/gr.772403","volume":"13","author":"PD Thomas","year":"2003","unstructured":"Thomas PD, Campbell MJ, Kejariwal A, Mi H, Karlak B, Daverman R, Diemer K, Muruganujan A, Narechania A: PANTHER: a library of protein families and subfamilies indexed by function. Genome Res. 2003, 13 (9): 2129-2141. 10.1101\/gr.772403.","journal-title":"Genome Res"},{"issue":"1","key":"424_CR35","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1023\/A:1011320027914","volume":"1","author":"KL Yap","year":"2000","unstructured":"Yap KL, Kim J, Truong K, Sherman M, Yuan T, Ikura M: Calmodulin target database. J Struct Funct Genomics. 2000, 1 (1): 8-14. 10.1023\/A:1011320027914.","journal-title":"J Struct Funct Genomics"},{"issue":"1","key":"424_CR36","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/nar\/28.1.15","volume":"28","author":"DA Benson","year":"2000","unstructured":"Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Rapp BA, Wheeler DL: GenBank. Nucleic Acids Res. 2000, 28 (1): 15-18. 10.1093\/nar\/28.1.15.","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"424_CR37","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1093\/nar\/28.1.10","volume":"28","author":"DL Wheeler","year":"2000","unstructured":"Wheeler DL, Chappey C, Lash AE, Leipe DD, Madden TL, Schuler GD, Tatusova TA, Rapp BA: Database resources of the National Center for Biotechnology Information. Nucleic Acids Res. 2000, 28 (1): 10-14. 10.1093\/nar\/28.1.10.","journal-title":"Nucleic Acids Res"},{"issue":"7","key":"424_CR38","doi-asserted-by":"publisher","first-page":"4354","DOI":"10.1073\/pnas.78.7.4354","volume":"78","author":"H Hidaka","year":"1981","unstructured":"Hidaka H, Sasaki Y, Tanaka T, Endo T, Ohno S, Fujii Y, Nagata T: N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation. Proc Natl Acad Sci U S A. 1981, 78 (7): 4354-4357. 10.1073\/pnas.78.7.4354.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"424_CR39","first-page":"571","volume":"20","author":"H Hidaka","year":"1981","unstructured":"Hidaka H, Asano M, Tanaka T: Activity-structure relationship of calmodulin antagonists, Naphthalenesulfonamide derivatives. Mol Pharmacol. 1981, 20 (3): 571-578.","journal-title":"Mol Pharmacol"},{"issue":"2","key":"424_CR40","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/0014-5793(94)80487-7","volume":"342","author":"A Perianin","year":"1994","unstructured":"Perianin A, Pedruzzi E, Hakim J: W-7, a calmodulin antagonist, primes the stimulation of human neutrophil respiratory burst by formyl peptides and platelet-activating factor. FEBS Lett. 1994, 342 (2): 135-138. 10.1016\/0014-5793(94)80487-7.","journal-title":"FEBS Lett"},{"issue":"8","key":"424_CR41","doi-asserted-by":"crossref","first-page":"4315","DOI":"10.1016\/S0021-9258(19)39565-1","volume":"265","author":"H Tokumitsu","year":"1990","unstructured":"Tokumitsu H, Chijiwa T, Hagiwara M, Mizutani A, Terasawa M, Hidaka H: KN-62, 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazi ne, a specific inhibitor of Ca2+\/calmodulin-dependent protein kinase II. J Biol Chem. 1990, 265 (8): 4315-4320.","journal-title":"J Biol Chem"},{"key":"424_CR42","volume-title":"Leuk Res","author":"AD Schuler","year":"2006","unstructured":"Schuler AD, Si J, Mueller L, Simon JA, Collins SJ: KN-62 analogues as potent differentiating agents of HL-60 cells. Leuk Res. 2006"},{"issue":"6","key":"424_CR43","doi-asserted-by":"publisher","first-page":"H2311","DOI":"10.1152\/ajpheart.00932.2001","volume":"284","author":"JC Schneider","year":"2003","unstructured":"Schneider JC, El Kebir D, Chereau C, Lanone S, Huang XL, De Buys Roessingh AS, Mercier JC, Dall'Ava-Santucci J, Dinh-Xuan AT: Involvement of Ca2+\/calmodulin-dependent protein kinase II in endothelial NO production and endothelium-dependent relaxation. Am J Physiol Heart Circ Physiol. 2003, 284 (6): H2311-9.","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"5","key":"424_CR44","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1096\/fasebj.11.5.9141499","volume":"11","author":"AR Rhoads","year":"1997","unstructured":"Rhoads AR, Friedberg F: Sequence motifs for calmodulin recognition. Faseb J. 1997, 11 (5): 331-340.","journal-title":"Faseb J"},{"issue":"10","key":"424_CR45","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1007\/s00109-002-0370-2","volume":"80","author":"N Wettschureck","year":"2002","unstructured":"Wettschureck N, Offermanns S: Rho\/Rho-kinase mediated signaling in physiology and pathophysiology. J Mol Med. 2002, 80 (10): 629-638. 10.1007\/s00109-002-0370-2.","journal-title":"J Mol Med"},{"issue":"6916","key":"424_CR46","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1038\/nature01148","volume":"420","author":"S Etienne-Manneville","year":"2002","unstructured":"Etienne-Manneville S, Hall A: Rho GTPases in cell biology. Nature. 2002, 420 (6916): 629-635. 10.1038\/nature01148.","journal-title":"Nature"},{"issue":"3","key":"424_CR47","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/S1534-5807(02)00268-X","volume":"3","author":"E Manser","year":"2002","unstructured":"Manser E: Small GTPases take the stage. Dev Cell. 2002, 3 (3): 323-328. 10.1016\/S1534-5807(02)00268-X.","journal-title":"Dev Cell"},{"issue":"5","key":"424_CR48","doi-asserted-by":"publisher","first-page":"2698","DOI":"10.1074\/jbc.273.5.2698","volume":"273","author":"P Flynn","year":"1998","unstructured":"Flynn P, Mellor H, Palmer R, Panayotou G, Parker PJ: Multiple interactions of PRK1 with RhoA. Functional assignment of the Hr1 repeat motif. J Biol Chem. 1998, 273 (5): 2698-2705. 10.1074\/jbc.273.5.2698.","journal-title":"J Biol Chem"},{"issue":"Pt 1","key":"424_CR49","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1042\/bj3510095","volume":"351","author":"SP Davies","year":"2000","unstructured":"Davies SP, Reddy H, Caivano M, Cohen P: Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J. 2000, 351 (Pt 1): 95-105. 10.1042\/0264-6021:3510095.","journal-title":"Biochem J"},{"issue":"1","key":"424_CR50","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1080\/02681218880000031","volume":"26","author":"S Resto","year":"1988","unstructured":"Resto S, Rodriguez-del Valle N: Yeast cell cycle of Sporothrix schenckii. J Med Vet Mycol. 1988, 26 (1): 13-24. 10.1080\/02681218880000031.","journal-title":"J Med Vet Mycol"},{"issue":"6","key":"424_CR51","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1210\/er.2003-0008","volume":"24","author":"CR Kahl","year":"2003","unstructured":"Kahl CR, Means AR: Regulation of cell cycle progression by calcium\/calmodulin-dependent pathways. Endocr Rev. 2003, 24 (6): 719-736. 10.1210\/er.2003-0008.","journal-title":"Endocr Rev"},{"issue":"3","key":"424_CR52","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1080\/00362178585380311","volume":"23","author":"S Betancourt","year":"1985","unstructured":"Betancourt S, Torres-Bauza LJ, Rodriguez-Del Valle N: Molecular and cellular events during the yeast to mycelium transition in Sporothrix schenckii. Sabouraudia. 1985, 23 (3): 207-218.","journal-title":"Sabouraudia"},{"key":"424_CR53","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1007\/978-1-4615-5873-6_21","volume":"2","author":"NN Nanthakumar","year":"1996","unstructured":"Nanthakumar NN, Dayton JS, Means AR: Role of Ca++\/calmodulin binding proteins in Aspergillus nidulans cell cycle regulation. Prog Cell Cycle Res. 1996, 2: 217-228.","journal-title":"Prog Cell Cycle Res"},{"issue":"20","key":"424_CR54","doi-asserted-by":"publisher","first-page":"10803","DOI":"10.1073\/pnas.93.20.10803","volume":"93","author":"H Enslen","year":"1996","unstructured":"Enslen H, Tokumitsu H, Stork PJ, Davis RJ, Soderling TR: Regulation of mitogen-activated protein kinases by a calcium\/calmodulin-dependent protein kinase cascade. Proc Natl Acad Sci U S A. 1996, 93 (20): 10803-10808. 10.1073\/pnas.93.20.10803.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"424_CR55","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/S0968-0004(99)01383-3","volume":"24","author":"TR Soderling","year":"1999","unstructured":"Soderling TR: The Ca-calmodulin-dependent protein kinase cascade. Trends Biochem Sci. 1999, 24 (6): 232-236. 10.1016\/S0968-0004(99)01383-3.","journal-title":"Trends Biochem Sci"},{"issue":"10","key":"424_CR56","doi-asserted-by":"publisher","first-page":"1511","DOI":"10.1091\/mbc.7.10.1511","volume":"7","author":"JS Dayton","year":"1996","unstructured":"Dayton JS, Means AR: Ca(2+)\/calmodulin-dependent kinase is essential for both growth and nuclear division in Aspergillus nidulans. Mol Biol Cell. 1996, 7 (10): 1511-1519.","journal-title":"Mol Biol Cell"},{"issue":"6","key":"424_CR57","doi-asserted-by":"publisher","first-page":"3223","DOI":"10.1074\/jbc.272.6.3223","volume":"272","author":"JS Dayton","year":"1997","unstructured":"Dayton JS, Sumi M, Nanthakumar NN, Means AR: Expression of a constitutively active Ca2+\/calmodulin-dependent kinase in Aspergillus nidulans spores prevents germination and entry into the cell cycle. J Biol Chem. 1997, 272 (6): 3223-3230. 10.1074\/jbc.272.6.3223.","journal-title":"J Biol Chem"},{"issue":"8","key":"424_CR58","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1248\/bpb.27.1281","volume":"27","author":"T Sato","year":"2004","unstructured":"Sato T, Ueno Y, Watanabe T, Mikami T, Matsumoto T: Role of Ca2+\/calmodulin signaling pathway on morphological development of Candida albicans. Biol Pharm Bull. 2004, 27 (8): 1281-1284. 10.1248\/bpb.27.1281.","journal-title":"Biol Pharm Bull"},{"issue":"3","key":"424_CR59","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1016\/S1087-1845(03)00044-6","volume":"39","author":"MJ de Carvalho","year":"2003","unstructured":"de Carvalho MJ, Amorim Jesuino RS, Daher BS, Silva-Pereira I, de Freitas SM, Soares CM, Felipe MS: Functional and genetic characterization of calmodulin from the dimorphic and pathogenic fungus Paracoccidioides brasiliensis. Fungal Genet Biol. 2003, 39 (3): 204-210. 10.1016\/S1087-1845(03)00044-6.","journal-title":"Fungal Genet Biol"},{"issue":"2","key":"424_CR60","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1080\/mmy.38.2.109.121","volume":"38","author":"N Delgado","year":"2000","unstructured":"Delgado N, Rodriguez-del Valle N: Presence of a pertussis toxin-sensitive G protein alpha subunit in Sporothrix schenckii. Med Mycol. 2000, 38 (2): 109-121. 10.1080\/714030929.","journal-title":"Med Mycol"},{"key":"424_CR61","volume-title":"Methods in Yeast Genetics","author":"F Sherman","year":"1986","unstructured":"Sherman F, Fink GR, Hicks JB: Methods in Yeast Genetics. 1986, Cold Spring Harbor, NY , Cold Spring Harbor Laboratory Press"},{"issue":"1","key":"424_CR62","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","volume":"162","author":"P Chomczynski","year":"1987","unstructured":"Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987, 162 (1): 156-159. 10.1016\/0003-2697(87)90021-2.","journal-title":"Anal Biochem"},{"issue":"13","key":"424_CR63","doi-asserted-by":"publisher","first-page":"3784","DOI":"10.1093\/nar\/gkg563","volume":"31","author":"E Gasteiger","year":"2003","unstructured":"Gasteiger E, Gattiker A, Hoogland C, Ivanyi I, Appel RD, Bairoch A: ExPASy: The proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res. 2003, 31 (13): 3784-3788. 10.1093\/nar\/gkg563.","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"424_CR64","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"SF Altschul","year":"1990","unstructured":"Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215 (3): 403-410.","journal-title":"J Mol Biol"},{"issue":"6","key":"424_CR65","doi-asserted-by":"publisher","first-page":"1692","DOI":"10.1093\/nar\/gkl091","volume":"34","author":"IM Wallace","year":"2006","unstructured":"Wallace IM, O'Sullivan O, Higgins DG, Notredame C: M-Coffee: combining multiple sequence alignment methods with T-Coffee. Nucleic Acids Res. 2006, 34 (6): 1692-1699. 10.1093\/nar\/gkl091.","journal-title":"Nucleic Acids Res"},{"key":"424_CR66","volume-title":"GeneDoc: Analysis and Visualization of Genetic Variation (http:\/\/www.nrbsc.org\/gfx\/genedoc\/index.html)","author":"KB Nicholas","year":"1997","unstructured":"Nicholas KB, Nicholas H.B. Jr., Deerfield DW: GeneDoc: Analysis and Visualization of Genetic Variation (http:\/\/www.nrbsc.org\/gfx\/genedoc\/index.html). 1997, Pittsburg Supercomputing Center, National Resource for Biomedical Supercomputing"},{"issue":"4","key":"424_CR67","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1093\/oxfordjournals.molbev.a026334","volume":"17","author":"J Castresana","year":"2000","unstructured":"Castresana J: Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000, 17 (4): 540-552.","journal-title":"Mol Biol Evol"},{"key":"424_CR68","volume-title":"Phylip (Phylogeny Inference Package) version 3.6 (http:\/\/evolution.genetics.washington.edu\/phylip.html)","author":"J Felsenstein","year":"2005","unstructured":"Felsenstein J: Phylip (Phylogeny Inference Package) version 3.6 (http:\/\/evolution.genetics.washington.edu\/phylip.html). 2005, Department of Genome Sciences, University of Washington"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2180-7-107.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T01:32:58Z","timestamp":1630459978000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/1471-2180-7-107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,11,29]]},"references-count":68,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["424"],"URL":"https:\/\/doi.org\/10.1186\/1471-2180-7-107","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,11,29]]},"assertion":[{"value":"2 April 2007","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 November 2007","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 November 2007","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"107"}}