{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T05:56:52Z","timestamp":1776491812243,"version":"3.51.2"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Pseudomonas aeruginosa<\/jats:italic> frequently colonizes and is responsible for severe ventilator-associated pneumonia in intubated patients. A quorum-sensing (QS) circuit, depending on the production of the two QS-signaling molecules (autoinducers, AIs) 3-oxo-C<jats:sub>12<\/jats:sub>-HSL and C<jats:sub>4<\/jats:sub>-HSL, regulates the production by <jats:italic>P. aeruginosa<\/jats:italic> of several virulence factors and is required for biofilm formation. Therefore QS-inhibition has been suggested as a new target for preventive and\/or therapeutic strategies. However the precise role of QS during colonization and subsequent infections of intubated patients remains unclear.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We wondered whether QS is active during colonization of intubated patients, and whether <jats:italic>P. aeruginosa<\/jats:italic> isolates growing inside the biofilm covering the intubation devices and those resident in the lungs of colonized patients differ in their QS-dependent phenotypes. We collected the intubation devices of eight patients colonized by <jats:italic>P. aeruginosa<\/jats:italic>. We detected 3-oxo-C<jats:sub>12<\/jats:sub>-HSL on eight, and C<jats:sub>4<\/jats:sub>-HSL on six of these devices. In three of these patients we also obtained <jats:italic>P. aeruginosa<\/jats:italic> isolates from tracheal aspirates at the time of extubation (n = 18), as well as isolates from the intubation devices (n = 25). We genotyped these isolates, quantified their AIs production, and determined three QS-dependent phenotypes (adherence capacity, biofilm and elastase production). The production of 3-oxo-C<jats:sub>12<\/jats:sub>-HSL was consistently increased for isolates from the intubation devices, whereas the production of C<jats:sub>4<\/jats:sub>-HSL was significantly higher for isolates from tracheal aspirates. Isolates from tracheal aspirates produced significantly higher amounts of elastase but less biofilm, and had a marginally reduced adhesion capacity than isolates from the intubation devices. Levels of 3-oxo-C<jats:sub>12<\/jats:sub>-HSL and elastase production correlated statistically for tracheal intubation isolates, whereas levels of 3-oxo-C<jats:sub>12<\/jats:sub>-HSL production and adhesion ability, as well as biofilm production, correlated weakly amongst intubation device isolates.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Our findings demonstrate that autoinducers are produced during the colonization of intubated patients by <jats:italic>P. aeruginosa<\/jats:italic>. The microenvironment, in which <jats:italic>P. aeruginosa<\/jats:italic> grows, may select for bacteria with different capacities to produce autoinducers and certain QS-dependent phenotypes. QS-inhibition might therefore affect differently isolates growing inside the biofilm covering intubation devices and those resident in the lungs.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-7-33","type":"journal-article","created":{"date-parts":[[2007,5,2]],"date-time":"2007-05-02T15:34:50Z","timestamp":1178120090000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Autoinducer production and quorum-sensing dependent phenotypes of Pseudomonas aeruginosa vary according to isolation site during colonization of intubated patients"],"prefix":"10.1186","volume":"7","author":[{"given":"Sabine","family":"Favre-Bont\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Chamot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thilo","family":"K\u00f6hler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques-A","family":"Romand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"van Delden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2007,4,18]]},"reference":[{"key":"350_CR1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0272-5231(21)00980-1","volume":"16","author":"M Dunn","year":"1995","unstructured":"Dunn M, Wunderink RG: Ventilator-associated pneumonia caused by Pseudomonas infection. Clin Chest Med. 1995, 16: 95-","journal-title":"Clin Chest Med"},{"issue":"3","key":"350_CR2","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/S0272-5231(21)00800-5","volume":"12","author":"SA Levine","year":"1991","unstructured":"Levine SA, Niederman MS: The impact of tracheal intubation on host defenses and risks for nosocomial pneumonia. Clin Chest Med. 1991, 12 (3): 523-","journal-title":"Clin Chest Med"},{"issue":"5418","key":"350_CR3","doi-asserted-by":"publisher","first-page":"1318","DOI":"10.1126\/science.284.5418.1318","volume":"284","author":"JW Costerton","year":"1999","unstructured":"Costerton JW, Stewart PS, Greenberg EP: Bacterial biofilms: a common cause of persistent infections. Science. 1999, 284 (5418): 1318-10.1126\/science.284.5418.1318.","journal-title":"Science"},{"key":"350_CR4","doi-asserted-by":"publisher","first-page":"551","DOI":"10.3201\/eid0404.980405","volume":"4","author":"C Van Delden","year":"1998","unstructured":"Van Delden C, Iglewski BH: Cell-to-cell signaling and Pseudomonas aeruginosa infections. Emerg Infect Dis. 1998, 4: 551-","journal-title":"Emerg Infect Dis"},{"key":"350_CR5","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1111\/j.1365-2958.1995.mmi_17020333.x","volume":"17","author":"A Latifi","year":"1995","unstructured":"Latifi A, Winson MK, Foglino M, Bycroft BW, Stewart GS, Lazdunski A, Williams P: Multiple homologues of LuxR and LuxI control expression of virulence determinants and secondary metabolites through quorum sensing in Pseudomonas aeruginosa PAO1. Mol Microbiol. 1995, 17: 333-10.1111\/j.1365-2958.1995.mmi_17020333.x.","journal-title":"Mol Microbiol"},{"issue":"5111","key":"350_CR6","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1126\/science.8493556","volume":"260","author":"L Passador","year":"1993","unstructured":"Passador L, Cook JM, Gambello MJ, Rust L, Iglewski BH: Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication. Science. 1993, 260 (5111): 1127-10.1126\/science.8493556.","journal-title":"Science"},{"key":"350_CR7","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1126\/science.280.5361.295","volume":"280","author":"DG Davies","year":"1998","unstructured":"Davies DG, Parsek MR, Pearson JP, Iglewski BH, Costerton JW, Greenberg EP: The involvement of cell-to cell signals in the development of bacterial biofilm. Science. 1998, 280: 295-10.1126\/science.280.5361.295.","journal-title":"Science"},{"key":"350_CR8","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1093\/jac\/dkg397","volume":"52","author":"S Favre-Bont\u00e9","year":"2003","unstructured":"Favre-Bont\u00e9 S, K\u00f6hler T, Van Delden C: Biofilm formation by Pseudomonas aeruginosa : role of the C4-HSL cell-to-cell signal and inhibition by azithromycin . J Antimicrob Chemother. 2003, 52: 598-604. 10.1093\/jac\/dkg397.","journal-title":"J Antimicrob Chemother"},{"issue":"7","key":"350_CR9","doi-asserted-by":"publisher","first-page":"2066","DOI":"10.1128\/JB.185.7.2066-2079.2003","volume":"185","author":"M Schuster","year":"2003","unstructured":"Schuster M, Lostroh CP, Ogi T, Greenberg EP: Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: a transcriptome analysis. J Bacteriol. 2003, 185 (7): 2066-10.1128\/JB.185.7.2066-2079.2003.","journal-title":"J Bacteriol"},{"key":"350_CR10","doi-asserted-by":"publisher","first-page":"2080","DOI":"10.1128\/JB.185.7.2080-2095.2003","volume":"185","author":"VE Wagner","year":"2003","unstructured":"Wagner VE, Bushnell D, Passador L, Brooks AI, Iglewski BH: Microarray analysis of Pseudomonas aeruginosa quorum-sensing regulons: effects of growth phase and environment. J Bacteriol. 2003, 185: 2080-10.1128\/JB.185.7.2080-2095.2003.","journal-title":"J Bacteriol"},{"key":"350_CR11","doi-asserted-by":"publisher","first-page":"1721","DOI":"10.1016\/S1286-4579(00)01327-7","volume":"2","author":"KP Rumbaugh","year":"2000","unstructured":"Rumbaugh KP, Griswold JA, Hamood AN: The role of quorum sensing in the in vivo virulence of Pseudomonas aeruginosa. Microbes Infect. 2000, 2: 1721-10.1016\/S1286-4579(00)01327-7.","journal-title":"Microbes Infect"},{"key":"350_CR12","first-page":"273","volume":"184","author":"O Geisenberger","year":"2000","unstructured":"Geisenberger O, Givskov M, Riedel K, Hoiby N, T\u00fcmmler B, Eberl L: Production of N-acyl-L-homoserine lactones by P. aeruginosa isolates from chronic lung infections associated with cystic fibrosis. FEMS Microbiol Lett. 2000, 184: 273-","journal-title":"FEMS Microbiol Lett"},{"key":"350_CR13","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1038\/35037627","volume":"407","author":"PK Singh","year":"2000","unstructured":"Singh PK, Schaefer AL, Parsek MR, Moninger TO, Welsh MJ, Greenberg EP: Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms. Nature. 2000, 407: 762-10.1038\/35037627.","journal-title":"Nature"},{"issue":"3","key":"350_CR14","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1006\/mpat.2001.0487","volume":"32","author":"S Favre-Bont\u00e9","year":"2002","unstructured":"Favre-Bont\u00e9 S, Pache JC, Robert J, Blanc D, Pech\u00e8re JC, Van Delden C: Detection of Pseudomonas aeruginosa cell-to-cell signals in lung tissue of cystic fibrosis patients. Microb Pathog. 2002, 32 (3): 143-10.1006\/mpat.2001.0487.","journal-title":"Microb Pathog"},{"issue":"4","key":"350_CR15","doi-asserted-by":"publisher","first-page":"1783","DOI":"10.1128\/IAI.70.4.1783-1790.2002","volume":"70","author":"DL Erickson","year":"2002","unstructured":"Erickson DL, Endersby R, Kirkham A, Stuber K, Vollman DD, Rabin HR, Mitchell I, Storey DG: Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis. Infect Immun. 2002, 70 (4): 1783-10.1128\/IAI.70.4.1783-1790.2002.","journal-title":"Infect Immun"},{"issue":"1","key":"350_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1574-6968.2002.tb11019.x","volume":"207","author":"B Middleton","year":"2002","unstructured":"Middleton B, Rodgers HC, Camara M, Knox AJ, Williams P, Hardman A: Direct detection of N-acylhomoserine lactones in cystic fibrosis sputum. FEMS Microbiol Lett. 2002, 207 (1): 1-10.1111\/j.1574-6968.2002.tb11019.x.","journal-title":"FEMS Microbiol Lett"},{"issue":"5","key":"350_CR17","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1136\/thorax.58.5.444","volume":"58","author":"C Ward","year":"2003","unstructured":"Ward C, Camara M, Forrest I, Rutherford R, Pritchard G, Daykin M, Hardman A, de Soyza A, Fisher AJ, Williams P, Corris PA: Preliminary findings of quorum signal molecules in clinically stable lung allograft recipients. Thorax. 2003, 58 (5): 444-10.1136\/thorax.58.5.444.","journal-title":"Thorax"},{"issue":"2","key":"350_CR18","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1128\/JCM.42.2.554-562.2004","volume":"42","author":"V Denervaud","year":"2004","unstructured":"Denervaud V, TuQuoc P, Blanc D, Favre-Bont\u00e9 S, Krishnapillai V, Reimmann C, Haas D, Van Delden C: Characterization of cell-to-cell signaling-deficient Pseudomonas aeruginosa strains colonizing intubated patients. J Clin Microbiol. 2004, 42 (2): 554-10.1128\/JCM.42.2.554-562.2004.","journal-title":"J Clin Microbiol"},{"key":"350_CR19","doi-asserted-by":"crossref","first-page":"3486","DOI":"10.1128\/AEM.64.9.3486-3490.1998","volume":"64","author":"DJ Stickler","year":"1998","unstructured":"Stickler DJ, Morris NS, McLean RJ, Fuqua C: Biofilms on indwelling urethral catheters produce quorum-sensing signal molecules in situ and in vitro . Appl Environ Microbiol. 1998, 64: 3486-","journal-title":"Appl Environ Microbiol"},{"key":"350_CR20","volume-title":"Mol Microbiol","author":"JD Shrout","year":"2006","unstructured":"Shrout JD, Chopp DL, Just CL, Hentzer M, Givskov M, Parsek MR: The impact of quorum sensing and swarming motility on Pseudomonas aeruginosa biofilm formation is nutritionally conditional. Mol Microbiol. 2006"},{"issue":"6","key":"350_CR21","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.1128\/IAI.66.6.2521-2528.1998","volume":"66","author":"DG Storey","year":"1998","unstructured":"Storey DG, Ujack EE, Rabin HR, Mitchell I: Pseudomonas aeruginosa lasR transcription correlates with the transcription of lasA, lasB, and toxA in chronic lung infections associated with cystic fibrosis. Infect Immun. 1998, 66 (6): 2521-","journal-title":"Infect Immun"},{"key":"350_CR22","doi-asserted-by":"publisher","first-page":"7222","DOI":"10.1128\/JB.185.24.7222-7230.2003","volume":"185","author":"S Cabrol","year":"2003","unstructured":"Cabrol S, Olliver A, Pier GB, Andremont A, Ruimy R: Transcription of quorum-sensing system genes in clinical and environmental isolates of Pseudomonas aeruginosa. J Bacteriol. 2003, 185: 7222-10.1128\/JB.185.24.7222-7230.2003.","journal-title":"J Bacteriol"},{"issue":"7","key":"350_CR23","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1164\/ajrccm.165.7.2105078","volume":"165","author":"J Chastre","year":"2002","unstructured":"Chastre J, Fagon JY: Ventilator-associated pneumonia. Am J Respir Crit Care Med. 2002, 165 (7): 867-","journal-title":"Am J Respir Crit Care Med"},{"issue":"2 Suppl","key":"350_CR24","doi-asserted-by":"publisher","first-page":"1S","DOI":"10.1378\/chest.108.2_Supplement.1S","volume":"108","author":"DE Craven","year":"1995","unstructured":"Craven DE, Steger KA: Epidemiology of nosocomial pneumonia. New perspectives on an old disease. Chest. 1995, 108 (2 Suppl): 1S-","journal-title":"Chest"},{"issue":"1","key":"350_CR25","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1164\/ajrccm.154.1.8680665","volume":"154","author":"J Rello","year":"1996","unstructured":"Rello J, Sonora R, Jubert P, Artigas A, Rue M, Valles J: Pneumonia in intubated patients: role of respiratory airway care. Am J Respir Crit Care Med. 1996, 154 (1): 111-","journal-title":"Am J Respir Crit Care Med"},{"issue":"4","key":"350_CR26","doi-asserted-by":"publisher","first-page":"1865","DOI":"10.1128\/AEM.67.4.1865-1873.2001","volume":"67","author":"TR De Kievit","year":"2001","unstructured":"De Kievit TR, Gillis R, Marx S, Brown C, Iglewski BH: Quorum-sensing genes in Pseudomonas aeruginosa biofilms: their role and expression patterns. Appl Environ Microbiol. 2001, 67 (4): 1865-10.1128\/AEM.67.4.1865-1873.2001.","journal-title":"Appl Environ Microbiol"},{"issue":"4","key":"350_CR27","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.1128\/jb.184.4.1140-1154.2002","volume":"184","author":"K Sauer","year":"2002","unstructured":"Sauer K, Camper AK, Ehrlich GD, Costerton JW, Davies DG: Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm. J Bacteriol. 2002, 184 (4): 1140-10.1128\/jb.184.4.1140-1154.2002.","journal-title":"J Bacteriol"},{"issue":"3","key":"350_CR28","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1128\/JB.185.3.1027-1036.2003","volume":"185","author":"ME Davey","year":"2003","unstructured":"Davey ME, Caiazza NC, O'Toole GA: Rhamnolipid surfactant production affects biofilm architecture in Pseudomonas aeruginosa PAO1. J Bacteriol. 2003, 185 (3): 1027-10.1128\/JB.185.3.1027-1036.2003.","journal-title":"J Bacteriol"},{"issue":"6882","key":"350_CR29","doi-asserted-by":"publisher","first-page":"740","DOI":"10.1038\/416740a","volume":"416","author":"E Drenkard","year":"2002","unstructured":"Drenkard E, Ausubel FM: Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation. Nature. 2002, 416 (6882): 740-10.1038\/416740a.","journal-title":"Nature"},{"issue":"6","key":"350_CR30","doi-asserted-by":"publisher","first-page":"1930","DOI":"10.1128\/AAC.45.6.1930-1933.2001","volume":"45","author":"K Tateda","year":"2001","unstructured":"Tateda K, Comte R, Pech\u00e8re JC, K\u00f6hler T, Yamaguchi K, Van Delden C: Azithromycin inhibits quorum sensing in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2001, 45 (6): 1930-1933. 10.1128\/AAC.45.6.1930-1933.2001.","journal-title":"Antimicrob Agents Chemother"},{"issue":"12","key":"350_CR31","doi-asserted-by":"publisher","first-page":"5842","DOI":"10.1128\/JCM.42.12.5842-5845.2004","volume":"42","author":"RJ Gillis","year":"2004","unstructured":"Gillis RJ, Iglewski BH: Azithromycin retards Pseudomonas aeruginosa biofilm formation. J Clin Microbiol. 2004, 42 (12): 5842-10.1128\/JCM.42.12.5842-5845.2004.","journal-title":"J Clin Microbiol"},{"issue":"3","key":"350_CR32","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1128\/iai.62.3.897-903.1994","volume":"62","author":"SE West","year":"1994","unstructured":"West SE, Kaye SA, Hamood AN, Iglewski BH: Characterization of Pseudomonas aeruginosa mutants that are deficient in exotoxin A synthesis and are altered in expression of regA, a positive regulator of exotoxin A. Infect Immun. 1994, 62 (3): 897-","journal-title":"Infect Immun"},{"issue":"4","key":"350_CR33","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1128\/iai.60.4.1329-1335.1992","volume":"60","author":"JM Terry","year":"1992","unstructured":"Terry JM, Pina SE, Mattingly SJ: Role of energy metabolism in conversion of nonmucoid Pseudomonas aeruginosa to the mucoid phenotype. Infect Immun. 1992, 60 (4): 1329-","journal-title":"Infect Immun"},{"issue":"1","key":"350_CR34","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1007\/BF01706421","volume":"18","author":"P Mahul","year":"1992","unstructured":"Mahul P, Auboyer C, Jospe R, Ros A, Guerin C, el Khouri Z, Galliez M, Dumont A, Gaudin O: Prevention of nosocomial pneumonia in intubated patients: respective role of mechanical subglottic secretions drainage and stress ulcer prophylaxis. Intensive Care Med. 1992, 18 (1): 20-10.1007\/BF01706421.","journal-title":"Intensive Care Med"},{"issue":"9","key":"350_CR35","doi-asserted-by":"crossref","first-page":"2014","DOI":"10.1128\/jcm.27.9.2014-2018.1989","volume":"27","author":"TJ Inglis","year":"1989","unstructured":"Inglis TJ, Millar MR, Jones JG, Robinson DA: Tracheal tube biofilm as a source of bacterial colonization of the lung. J Clin Microbiol. 1989, 27 (9): 2014-","journal-title":"J Clin Microbiol"},{"issue":"4","key":"350_CR36","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1378\/chest.108.4.1049","volume":"108","author":"TJ Inglis","year":"1995","unstructured":"Inglis TJ, Lim TM, Ng ML, Tang EK, Hui KP: Structural features of tracheal tube biofilm formed during prolonged mechanical ventilation. Chest. 1995, 108 (4): 1049-","journal-title":"Chest"},{"issue":"3","key":"350_CR37","doi-asserted-by":"publisher","first-page":"179","DOI":"10.7326\/0003-4819-122-3-199502010-00004","volume":"122","author":"J Valles","year":"1995","unstructured":"Valles J, Artigas A, Rello J, Bonsoms N, Fontanals D, Blanch L, Fernandez R, Baigorri F, Mestre J: Continuous aspiration of subglottic secretions in preventing ventilator- associated pneumonia. Ann Intern Med. 1995, 122 (3): 179-","journal-title":"Ann Intern Med"},{"issue":"18","key":"350_CR38","doi-asserted-by":"crossref","first-page":"5756","DOI":"10.1128\/jb.179.18.5756-5767.1997","volume":"179","author":"JP Pearson","year":"1997","unstructured":"Pearson JP, Pesci EC, Iglewski BH: Role of Pseudomonas aeruginosa las and rhl quorum-sensing systems in the control of elastase and rhamnolipid biosynthesis genes. J Bacteriol. 1997, 179 (18): 5756-","journal-title":"J Bacteriol"},{"issue":"18","key":"350_CR39","doi-asserted-by":"publisher","first-page":"5376","DOI":"10.1128\/JB.183.18.5376-5384.2001","volume":"183","author":"C Van Delden","year":"2001","unstructured":"Van Delden C, Comte R, Bally M: Stringent response activates quorum sensing and modulates cell density-dependent gene expression in Pseudomonas aeruginosa. J Bacteriol. 2001, 183 (18): 5376-10.1128\/JB.183.18.5376-5384.2001.","journal-title":"J Bacteriol"},{"issue":"5","key":"350_CR40","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1128\/jcm.34.5.1129-1135.1996","volume":"34","author":"E Mahenthiralingam","year":"1996","unstructured":"Mahenthiralingam E, Campbell ME, Foster J, Lam JS, Speert DP: Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol. 1996, 34 (5): 1129-","journal-title":"J Clin Microbiol"},{"issue":"9","key":"350_CR41","doi-asserted-by":"crossref","first-page":"4499","DOI":"10.1128\/IAI.66.9.4499-4502.1998","volume":"66","author":"C Van Delden","year":"1998","unstructured":"Van Delden C, Pesci EC, Pearson JP, Iglewski BH: Starvation selection restores elastase and rhamnolipid production in a Pseudomonas aeruginosa quorum-sensing mutant. Infect Immun. 1998, 66 (9): 4499-","journal-title":"Infect Immun"},{"issue":"18","key":"350_CR42","doi-asserted-by":"publisher","first-page":"5213","DOI":"10.1128\/JB.183.18.5213-5222.2001","volume":"183","author":"T K\u00f6hler","year":"2001","unstructured":"K\u00f6hler T, Van Delden C, Curty LK, Hamzehpour MM, Pech\u00e8re JC: Overexpression of the MexEF-OprN multidrug efflux system affects cell-to-cell signaling in Pseudomonas aeruginosa. J Bacteriol. 2001, 183 (18): 5213-5222. 10.1128\/JB.183.18.5213-5222.2001.","journal-title":"J Bacteriol"},{"issue":"2","key":"350_CR43","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1046\/j.1365-2958.1998.01062.x","volume":"30","author":"GA O'Toole","year":"1998","unstructured":"O'Toole GA, Kolter R: Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol. 1998, 30 (2): 295-10.1046\/j.1365-2958.1998.01062.x.","journal-title":"Mol Microbiol"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2180-7-33.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T10:08:56Z","timestamp":1630490936000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/1471-2180-7-33"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4,18]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["350"],"URL":"https:\/\/doi.org\/10.1186\/1471-2180-7-33","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4,18]]},"assertion":[{"value":"16 September 2006","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 April 2007","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 April 2007","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"33"}}