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Using antibodies raised with fusion proteins, we characterized four such hypothetical proteins encoded by two gene clusters (<jats:italic>Cpn0146-147<\/jats:italic>and<jats:italic>Cpn0284-285<\/jats:italic>) in the<jats:italic>C. pneumoniae<\/jats:italic>genome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cpn0146 and 0147 were detected in the inclusion membrane while Cpn0284 and 0285 inside inclusion and mainly associated with reticulate bodies although all four proteins contain an N-terminal bi-lobed hydrophobic region, a signature motif assigned to inclusion membrane proteins. These four hypothetical proteins were only detected in cells infected with<jats:italic>C. pneumoniae<\/jats:italic>but not other chlamydial species, with Cpn0147 at 6 hours and Cpn0146, 0284 &amp; 0285 at 24 hours after infection. Cpn0146 &amp; 147 but not Cpn0284 and 285 co-localized with a host cell endoplasmic reticulum marker, a property known to be possessed by some chlamydial inclusion membrane proteins, when expressed in the host cell cytosol via transgenes. However, the endoplasmic reticulum localization of the<jats:italic>C. pneumoniae<\/jats:italic>inclusion membrane proteins did not result in inhibition of the subsequent<jats:italic>C. pneumoniae<\/jats:italic>infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The hypothetical proteins Cpn0146 &amp; 0147 were localized in the<jats:italic>C. pneumoniae<\/jats:italic>inclusion membrane while Cpn0284 &amp; 0285 within the inclusion although all four were predicted to be Inc proteins, suggesting the need to experimentally characterize the predicted Inc proteins.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2180-7-38","type":"journal-article","created":{"date-parts":[[2007,5,16]],"date-time":"2007-05-16T06:13:28Z","timestamp":1179296008000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Characterization of hypothetical proteins Cpn0146, 0147, 0284 &amp; 0285 that are predicted to be in the Chlamydia pneumoniae inclusion membrane"],"prefix":"10.1186","volume":"7","author":[{"given":"Jianhua","family":"Luo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangchao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youmin","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjun","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangming","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,5,15]]},"reference":[{"issue":"5389","key":"355_CR1","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1126\/science.282.5389.754","volume":"282","author":"RS Stephens","year":"1998","unstructured":"Stephens RS, Kalman S, Lammel C, Fan J, Marathe R, Aravind L, Mitchell W, Olinger L, Tatusov RL, Zhao Q, Koonin EV, Davis RW: Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. 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