{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:05:30Z","timestamp":1789711530449,"version":"4.0.1"},"reference-count":59,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2007,7,2]],"date-time":"2007-07-02T00:00:00Z","timestamp":1183334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Little is known regarding the extent or targets of phosphorylation in mycoplasmas, yet in many other bacterial species phosphorylation is known to play an important role in signaling and regulation of cellular processes. To determine the prevalence of phosphorylation in mycoplasmas, we examined the CHAPS-soluble protein fractions of <jats:italic>Mycoplasma genitalium<\/jats:italic> and <jats:italic>Mycoplasma pneumoniae<\/jats:italic> by two-dimensional gel electrophoresis (2-DE), using a combination of Pro-Q Diamond phosphoprotein stain and <jats:sup>33<\/jats:sup>P labeling. Protein spots that were positive for phosphorylation were identified by peptide mass fingerprinting using MALDI-TOF-TOF mass spectrometry.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We identified a total of 24 distinct phosphoproteins, about 3% and 5% of the total protein complement in <jats:italic>M. pneumoniae<\/jats:italic> and <jats:italic>M. genitalium<\/jats:italic>, respectively, indicating that phosphorylation occurs with prevalence similar to many other bacterial species. Identified phosphoproteins include pyruvate dehydrogenase E1 alpha and beta subunits, enolase, heat shock proteins DnaK and GroEL, elongation factor Tu, cytadherence accessory protein HMW3, P65, and several hypothetical proteins. These proteins are involved in energy metabolism, carbohydrate metabolism, translation\/transcription and cytadherence. Interestingly, fourteen of the 24 phosphoproteins we identified (58%) were previously reported as putatively associated with a cytoskeleton-like structure that is present in the mycoplasmas, indicating a potential regulatory role for phosphorylation in this structure.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This study has shown that phosphorylation in mycoplasmas is comparable to that of other bacterial species. Our evidence supports a link between phosphorylation and cytadherence and\/or a cytoskeleton-like structure, since over half of the proteins identified as phosphorylated have been previously associated with these functions. This opens the door to further research into the purposes and mechanisms of phosphorylation for mycoplasmas.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-7-63","type":"journal-article","created":{"date-parts":[[2007,7,3]],"date-time":"2007-07-03T06:15:06Z","timestamp":1183443306000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae"],"prefix":"10.1186","volume":"7","author":[{"given":"Hsun-Cheng","family":"Su","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Clyde A","family":"Hutchison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgan C","family":"Giddings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,7,2]]},"reference":[{"key":"380_CR1","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1128\/CMR.17.4.697-728.2004","volume":"17","author":"KB Waites","year":"2004","unstructured":"Waites KB, Talkington DF: Mycoplasma pneumoniae and Its Role as a Human Pathogen. Clin Microbiol Rev. 2004, 17: 697-728.","journal-title":"Clin Microbiol Rev"},{"key":"380_CR2","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1126\/science.270.5235.397","volume":"270","author":"CM Fraser","year":"1995","unstructured":"Fraser CM, Gocayne JD, White O, Adams MD, Clayton RA, Fleischmann RD, Bult CJ, Kerlavage AR, Sutton G, Kelley JM, Fritchman RD, Weidman JF, Small KV, Sandusky M, Fuhrmann J, Nguyen D, Utterback TR, Saudek DM, Phillips CA, Merrick JM, Tomb JF, Dougherty BA, Bott KF, Hu PC, Lucier TS, Peterson SN, Smith HO, Hutchison CA, Venter JC: The minimal gene complement of Mycoplasma genitalium. Science. 1995, 270: 397-403.","journal-title":"Science"},{"key":"380_CR3","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1093\/nar\/25.4.701","volume":"25","author":"R Himmelreich","year":"1997","unstructured":"Himmelreich R, Plagens H, Hilbert H, Reiner B, Herrmann R: Comparative analysis of the genomes of the bacteria Mycoplasma pneumoniae and Mycoplasma genitalium. Nucleic Acids Res. 1997, 25: 701-712.","journal-title":"Nucleic Acids Res"},{"key":"380_CR4","doi-asserted-by":"publisher","first-page":"2165","DOI":"10.1126\/science.286.5447.2165","volume":"286","author":"CA Hutchison","year":"1999","unstructured":"Hutchison CA, Peterson SN, Gill SR, Cline RT, White O, Fraser CM, Smith HO, Venter JC: Global transposon mutagenesis and a minimal mycoplasma genome. Science. 1999, 286: 2165-2169.","journal-title":"Science"},{"key":"380_CR5","doi-asserted-by":"crossref","first-page":"7499","DOI":"10.1128\/jb.176.24.7499-7505.1994","volume":"176","author":"LB Dirksen","year":"1994","unstructured":"Dirksen LB, Krebes KA, Krause DC: Phosphorylation of cytadherence-accessory proteins in Mycoplasma pneumoniae . J Bacteriol. 1994, 176: 7499-7505.","journal-title":"J Bacteriol"},{"key":"380_CR6","doi-asserted-by":"publisher","first-page":"3765","DOI":"10.1002\/1522-2683(200011)21:17<3765::AID-ELPS3765>3.0.CO;2-6","volume":"21","author":"JT Regula","year":"2000","unstructured":"Regula JT, Ueberle B, Boguth G, G\u00f6rg A, Schn\u00f6lzer M, Herrmann R, Frank R: Towards a two-dimensional proteome map of Mycoplasma pneumoniae . Electrophoresis. 2000, 21: 3765-3780.","journal-title":"Electrophoresis"},{"key":"380_CR7","doi-asserted-by":"publisher","first-page":"26253","DOI":"10.1074\/jbc.M605010200","volume":"281","author":"S Halbedel","year":"2006","unstructured":"Halbedel S, Busse J, Schmidl SR, Stulke J: Regulatory protein phosphorylation in Mycoplasma pneumoniae . A PP2C-type phosphatase serves to dephosphorylate HPr(Ser-P). J Biol Chem. 2006, 281: 26253-26259.","journal-title":"J Biol Chem"},{"issue":"1","key":"380_CR8","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/S0300-9084(98)80055-2","volume":"80","author":"AJ Cozzone","year":"1998","unstructured":"Cozzone AJ: Post-translational modification of proteins by reversible phosphorylation in prokaryotes. Biochimie. 1998, 80 (1): 43-48.","journal-title":"Biochimie"},{"key":"380_CR9","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1128\/MMBR.62.4.1094-1156.1998","volume":"62","author":"S Razin","year":"1998","unstructured":"Razin S, Yogev D, Naot Y: Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev. 1998, 62: 1094-1156.","journal-title":"Microbiol Mol Biol Rev"},{"key":"380_CR10","doi-asserted-by":"crossref","first-page":"4571","DOI":"10.1128\/jb.177.15.4571-4574.1995","volume":"177","author":"KA Krebes","year":"1995","unstructured":"Krebes KA, Dirksen LB, Krause DC: Phosphorylation of Mycoplasma pneumoniae cytadherence-accessory proteins in cell extracts. J Bacteriol. 1995, 177: 4571-4574.","journal-title":"J Bacteriol"},{"key":"380_CR11","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1002\/pmic.200300434","volume":"3","author":"TH Steinberg","year":"2003","unstructured":"Steinberg TH, Agnew BJ, Gee KR, Leung W-Y, Goodman T, Schulenberg B, Hendrickson J, Beechem JM, Haugland RP, Patton WF: Global quantitative phosphoprotein analysis using multiplexed proteomics technology. Proteomics. 2003, 3: 1128-1144.","journal-title":"Proteomics"},{"key":"380_CR12","doi-asserted-by":"publisher","first-page":"1637","DOI":"10.1002\/pmic.200300494","volume":"3","author":"AK Bendt","year":"2003","unstructured":"Bendt AK, Burkovski A, Schaffer S, Bott M, Farwick M, Hermann T: Towards a phosphoproteome map of Corynebacterium glutamicum . Proteomics. 2003, 3: 1637-1646.","journal-title":"Proteomics"},{"key":"380_CR13","doi-asserted-by":"publisher","first-page":"27251","DOI":"10.1074\/jbc.C300189200","volume":"278","author":"B Schulenberg","year":"2003","unstructured":"Schulenberg B, Aggeler R, Beechem J, Capaldi R, Patton W: Analysis of steady-state protein phosphorylation in mitochondria using a novel fluorescent phosphosensor dye. J Biol Chem. 2003, 278: 27251-27255.","journal-title":"J Biol Chem"},{"key":"380_CR14","unstructured":"Proteome 2D-PAGE Database. [http:\/\/tinyurl.com\/yv7cs9]"},{"key":"380_CR15","doi-asserted-by":"publisher","first-page":"3551","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2","volume":"20","author":"DN Perkins","year":"1999","unstructured":"Perkins DN, Pappin DJ, Creasy DM, Cottrell JS: Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. 1999, 20: 3551-3567.","journal-title":"Electrophoresis"},{"key":"380_CR16","doi-asserted-by":"publisher","first-page":"3278","DOI":"10.1093\/nar\/28.17.3278","volume":"28","author":"T Dandekar","year":"2000","unstructured":"Dandekar T, Huynen M, Regula JT, Ueberle B, Zimmermann CU, Andrade MA, Doerks T, S\u00e1nchez-Pulido L, Snel B, Suyama M, Yuan YP, Herrmann R, Bork P: Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames. Nucleic Acids Res. 2000, 28: 3278-3288.","journal-title":"Nucleic Acids Res"},{"key":"380_CR17","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1111\/j.1432-1033.1986.tb09858.x","volume":"159","author":"JC Cortay","year":"1986","unstructured":"Cortay JC, Rieul C, Duclos B, Cozzone AJ: Characterization of the phosphoproteins of Escherichia coli cells by electrophoretic analysis. Eur J Biochem. 1986, 159: 227-237.","journal-title":"Eur J Biochem"},{"issue":"Pt 4","key":"380_CR18","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.1099\/00221287-147-4-1045","volume":"147","author":"JT Regula","year":"2001","unstructured":"Regula JT, Boguth G, G\u00f6rg A, Hegermann J, Mayer F, Frank R, Herrmann R: Defining the mycoplasma 'cytoskeleton': the protein composition of the Triton X-100 insoluble fraction of the bacterium Mycoplasma pneumoniae determined by 2-D gel electrophoresis and mass spectrometry. Microbiology. 2001, 147 (Pt 4): 1045-1057.","journal-title":"Microbiology"},{"key":"380_CR19","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1038\/nbt0302-301","volume":"20","author":"SB Ficarro","year":"2002","unstructured":"Ficarro SB, McCleland ML, Stukenberg PT, Burke DJ, Ross MM, Shabanowitz J, Hunt DF, White FM: Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae . Nat Biotechnol. 2002, 20: 301-305.","journal-title":"Nat Biotechnol"},{"key":"380_CR20","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1002\/1615-9861(200102)1:2<194::AID-PROT194>3.0.CO;2-K","volume":"1","author":"H Kaufmann","year":"2001","unstructured":"Kaufmann H, Bailey J, Fussenegger M: Use of antibodies for detection of phosphorylated proteins separated by two-dimensional gel electrophoresis. Proteomics. 2001, 1: 194-199.","journal-title":"Proteomics"},{"key":"380_CR21","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1002\/pro.5560070111","volume":"7","author":"B Solow","year":"1998","unstructured":"Solow B, Bischoff KM, Zylka MJ, Kennelly PJ: Archael phosphoproteins. Identification of a hexosephosphate mutase and the alpha-subunit of succinyl-CoA synthetase in the extreme acidothermophile Sulfolobus solfataricus . Protein Sci. 1998, 7: 105-111.","journal-title":"Protein Sci"},{"key":"380_CR22","doi-asserted-by":"publisher","first-page":"1562","DOI":"10.1096\/fj.01-0102com","volume":"15","author":"VW Hu","year":"2001","unstructured":"Hu VW, Heikka DS, Dieffenbach PB, Ha L: Metabolic radiolabeling: experimental tool or Trojan horse? (35)S-Methionine induces DNA fragmentation and p53-dependent ROS production. Faseb J. 2001, 15: 1562-1568.","journal-title":"Faseb J"},{"key":"380_CR23","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1096\/fasebj.14.3.448","volume":"14","author":"VW Hu","year":"2000","unstructured":"Hu VW, Heikka DS: Radiolabeling revisited: metabolic labeling with (35)S-methionine inhibits cell cycle progression, proliferation, and survival. Faseb J. 2000, 14: 448-454.","journal-title":"Faseb J"},{"key":"380_CR24","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1126\/science.278.5338.631","volume":"278","author":"RL Tatusov","year":"1997","unstructured":"Tatusov RL, Koonin EV, Lipman DJ: A genomic perspective on protein families. Science. 1997, 278: 631-637.","journal-title":"Science"},{"key":"380_CR25","unstructured":"Clusters of Orthologous Groups of proteins (COGs). [http:\/\/www.ncbi.nlm.nih.gov\/COG\/]"},{"key":"380_CR26","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.1016\/0300-9084(89)90116-8","volume":"71","author":"HK Dannelly","year":"1989","unstructured":"Dannelly HK, Duclos B, Cozzone AJ, Reeves HC: Phosphorylation of Escherichia coli enolase. Biochimie. 1989, 71: 1095-1100.","journal-title":"Biochimie"},{"key":"380_CR27","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1111\/j.1365-2958.1995.tb02270.x","volume":"15","author":"P Freestone","year":"1995","unstructured":"Freestone P, Grant S, Toth I, Norris V: Identification of phosphoproteins in Escherichia coli . Mol Microbiol. 1995, 15: 573-580.","journal-title":"Mol Microbiol"},{"key":"380_CR28","doi-asserted-by":"publisher","first-page":"4822","DOI":"10.1021\/bi00135a012","volume":"31","author":"ES Robertson","year":"1992","unstructured":"Robertson ES, Nicholson AW: Phosphorylation of Escherichia coli translation initiation factors by the bacteriophage T7 protein kinase. Biochemistry. 1992, 31: 4822-4827.","journal-title":"Biochemistry"},{"key":"380_CR29","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1093\/nar\/26.1.382","volume":"26","author":"N Blom","year":"1998","unstructured":"Blom N, Kreegipuu A, Brunak S: PhosphoBase: a database of phosphorylation sites. Nucleic Acids Res. 1998, 26: 382-386.","journal-title":"Nucleic Acids Res"},{"key":"380_CR30","unstructured":"NetPhos. [http:\/\/cbs.dtu.dk\/services\/NetPhos]"},{"key":"380_CR31","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1111\/j.1574-6968.1996.tb08145.x","volume":"138","author":"M Seeger","year":"1996","unstructured":"Seeger M, Osorio G, Jerez CA: Phosphorylation of GroEL, DnaK and other proteins from Thiobacillus ferrooxidans grown under different conditions. FEMS Microbiol Lett. 1996, 138: 129-134.","journal-title":"FEMS Microbiol Lett"},{"key":"380_CR32","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1046\/j.1432-1327.2000.01183.x","volume":"267","author":"VC Wasinger","year":"2000","unstructured":"Wasinger VC, Pollack JD, Humphery-Smith I: The proteome of Mycoplasma genitalium . Chaps-soluble component. Eur J Biochem. 2000, 267: 1571-1582.","journal-title":"Eur J Biochem"},{"key":"380_CR33","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1007\/0-306-47606-1_22","volume-title":"Molecular biology and pathogenicity of mycoplasmas","author":"M Balish","year":"2002","unstructured":"Balish M, Karuse DC: Cytadherence and the cytoskeleton. Molecular biology and pathogenicity of mycoplasmas. Edited by: Razin S, Herrmann R. 2002, New York, NY: Kluver Academic\/Plenum Publishers, 491-518."},{"key":"380_CR34","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1007\/0-306-47606-1_6","volume-title":"Molecular biology and pathogenicity of mycoplasmas","author":"M Miyata","year":"2002","unstructured":"Miyata M: Cell division. Molecular biology and pathogenicity of mycoplasmas. Edited by: Razin S, Herrmann R. 2002, New York, NY: Kluver Academic\/Plenum Publishers, 117-130."},{"key":"380_CR35","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0188-4409(01)00335-6","volume":"33","author":"S Dhandayuthapani","year":"2002","unstructured":"Dhandayuthapani S, Rasmussen W, Baseman J: Stability of cytadherence-related proteins P140\/P110 in Mycoplasma genitalium requires MG218 and unidentified factors. Arch Med Res. 2002, 33: 1-5.","journal-title":"Arch Med Res"},{"key":"380_CR36","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1111\/j.1365-2958.1996.tb02613.x","volume":"20","author":"DC Krause","year":"1996","unstructured":"Krause DC: Mycoplasma pneumoniae cytadherence: unravelling the tie that binds. Mol Microbiol. 1996, 20: 247-253.","journal-title":"Mol Microbiol"},{"key":"380_CR37","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1574-6968.2001.tb10610.x","volume":"198","author":"D Krause","year":"2001","unstructured":"Krause D, Balish M: Structure, function, and assembly of the terminal organelle of Mycoplasma pneumoniae . FEMS Microbiol Lett. 2001, 198: 1-7.","journal-title":"FEMS Microbiol Lett"},{"key":"380_CR38","doi-asserted-by":"publisher","first-page":"1082","DOI":"10.1128\/JB.185.3.1082-1091.2003","volume":"185","author":"S Seto","year":"2003","unstructured":"Seto S, Miyata M: Attachment organelle formation represented by localization of cytadherence proteins and formation of the electron-dense core in wild-type and mutant strains of Mycoplasma pneumoniae . J Bacteriol. 2003, 185: 1082-1091.","journal-title":"J Bacteriol"},{"key":"380_CR39","doi-asserted-by":"publisher","first-page":"6944","DOI":"10.1128\/JB.186.20.6944-6955.2004","volume":"186","author":"T Kenri","year":"2004","unstructured":"Kenri T, Seto S, Horino A, Sasaki Y, Sasaki T, Miyata M: Use of Fluorescent-Protein Tagging To Determine the Subcellular Localization of Mycoplasma pneumoniae Proteins Encoded by the Cytadherence Regulatory Locus. J Bacteriol. 2004, 186: 6944-6955.","journal-title":"J Bacteriol"},{"key":"380_CR40","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/S0966-842X(97)01168-2","volume":"6","author":"DC Krause","year":"1998","unstructured":"Krause DC: Mycoplasma pneumoniae cytadherence: organization and assembly of the attachment organelle. Trends Microbiol. 1998, 6: 15-18.","journal-title":"Trends Microbiol"},{"key":"380_CR41","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1099\/00221287-146-3-741","volume":"146","author":"G Layh-Schmitt","year":"2000","unstructured":"Layh-Schmitt G, Podtelejnikov A, Mann M: Proteins complexed to the P1 adhesin of Mycoplasma pneumoniae . Microbiology. 2000, 146: 741-747.","journal-title":"Microbiology"},{"key":"380_CR42","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1128\/iai.24.2.468-475.1979","volume":"24","author":"EJ Hansen","year":"1979","unstructured":"Hansen EJ, Wilson RM, Baseman JB: Two-dimensional gel electrophoretic comparison of proteins from virulent and avirulent strains of Mycoplasma pneumoniae . Infect Immun. 1979, 24: 468-475.","journal-title":"Infect Immun"},{"key":"380_CR43","doi-asserted-by":"publisher","first-page":"2422","DOI":"10.1073\/pnas.74.6.2422","volume":"74","author":"JM Starger","year":"1977","unstructured":"Starger JM, Goldman RD: Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells. Proc Natl Acad Sci USA. 1977, 74: 2422-2426.","journal-title":"Proc Natl Acad Sci USA"},{"key":"380_CR44","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1046\/j.1365-2958.2002.03207.x","volume":"46","author":"SF Dallo","year":"2002","unstructured":"Dallo SF, Kannan TR, Blaylock MW, Baseman JB: Elongation factor Tu and E1 beta subunit of pyruvate dehydrogenase complex act as fibronectin binding proteins in Mycoplasma pneumoniae . Mol Microbiol. 2002, 46: 1041-1051.","journal-title":"Mol Microbiol"},{"key":"380_CR45","doi-asserted-by":"publisher","first-page":"1250","DOI":"10.1073\/pnas.75.3.1250","volume":"75","author":"BD Beck","year":"1978","unstructured":"Beck BD, Arscott PG, Jacobson A: Novel properties of bacterial elongation factor Tu. Proc Natl Acad Sci USA. 1978, 75: 1250-1254.","journal-title":"Proc Natl Acad Sci USA"},{"key":"380_CR46","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/S0021-9258(18)54193-4","volume":"268","author":"C Lippmann","year":"1993","unstructured":"Lippmann C, Lindschau C, Vijgenboom E, Schroder W, Bosch L, Erdmann V: Prokaryotic elongation factor Tu is phosphorylated in vivo . J Biol Chem. 1993, 268: 601-607.","journal-title":"J Biol Chem"},{"key":"380_CR47","doi-asserted-by":"crossref","first-page":"2668","DOI":"10.1128\/jb.179.8.2668-2677.1997","volume":"179","author":"DC Krause","year":"1997","unstructured":"Krause DC, Proft T, Hedreyda CT, Hilbert H, Plagens H, Herrmann R: Transposon mutagenesis reinforces the correlation between Mycoplasma pneumoniae cytoskeletal protein HMW2 and cytadherence. J Bacteriol. 1997, 179: 2668-2677.","journal-title":"J Bacteriol"},{"key":"380_CR48","unstructured":"MyHit. [http:\/\/myhits.isb-sib.ch\/cgi-bin\/motif_scan]"},{"issue":"1","key":"380_CR49","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1111\/j.1471-4159.1992.tb09313.x","volume":"58","author":"B Harrison","year":"1992","unstructured":"Harrison B, Mobley P: Phosphorylation of glial fibrillary acidic protein and vimentin by cytoskeletal-associated intermediate filament protein kinase activity in astrocytes. J Neurochem. 1992, 58 (1): 320-327.","journal-title":"J Neurochem"},{"key":"380_CR50","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1083\/jcb.91.2.537","volume":"91","author":"U Gobel","year":"1981","unstructured":"Gobel U, Speth V, Bredt W: Filamentous structures in adherent Mycoplasma pneumoniae cells treated with nonionic detergents. J Cell Biol. 1981, 91: 537-543.","journal-title":"J Cell Biol"},{"key":"380_CR51","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/B978-012583805-4\/50005-4","volume-title":"Molecular and diagnostic procedures in mycoplasmology","author":"JG Tully","year":"1995","unstructured":"Tully JG: Culture medium formulation for primary isolation and maintenance of mollicutes. Molecular and diagnostic procedures in mycoplasmology. Edited by: Tully R. 1995, San Diego, CA: Academic Press, 1: 33-39."},{"key":"380_CR52","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-254.","journal-title":"Anal Biochem"},{"key":"380_CR53","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.1016\/0092-8674(77)90176-3","volume":"12","author":"PZ O'Farrell","year":"1977","unstructured":"O'Farrell PZ, Goodman HM, O'Farrell PH: High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977, 12: 1133-1141.","journal-title":"Cell"},{"key":"380_CR54","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V","volume":"21","author":"A G\u00f6rg","year":"2000","unstructured":"G\u00f6rg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W: The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis. 2000, 21: 1037-1053.","journal-title":"Electrophoresis"},{"issue":"11","key":"380_CR55","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1002\/elps.1150091103","volume":"9","author":"A G\u00f6rg","year":"1988","unstructured":"G\u00f6rg A, Postel W, Domscheit A, G\u00fcnther S: Two-dimensional electrophoresis with immobilized pH gradients of leaf proteins from barley (Hordeum vulgare): method, reproducibility and genetic aspects. Electrophoresis. 1988, 9 (11): 681-92.","journal-title":"Electrophoresis"},{"key":"380_CR56","unstructured":"ImageMaster 2D. [http:\/\/ca.expasy.org\/melanie\/2DImageAnalysisViewer.html]"},{"key":"380_CR57","first-page":"117","volume-title":"Methods in Molecular Biology","author":"C Parker","year":"2005","unstructured":"Parker C, Warren M, Loiselle D, Dicheva N, Scarlett C, Borchers C: Identification of components of protein complexes. Methods in Molecular Biology. Edited by: Totowa, NJ: Humana Press. 2005, Patterson W, 117-151."},{"key":"380_CR58","unstructured":"Mascot. [http:\/\/www.matrixscience.com\/search_form_select.html]"},{"key":"380_CR59","unstructured":"FindMod. [http:\/\/us.expasy.org\/tools\/findmod\/]"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2180-7-63.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1471-2180-7-63\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2180-7-63.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T01:39:16Z","timestamp":1630460356000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/1471-2180-7-63"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,2]]},"references-count":59,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["380"],"URL":"https:\/\/doi.org\/10.1186\/1471-2180-7-63","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,2]]},"assertion":[{"value":"21 November 2006","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2007","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2007","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"63"}}