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Over recent years the Bcc have emerged as problematic pathogens of the CF lung. <jats:italic>Pseudomonas aeruginosa<\/jats:italic> is another important CF pathogen. It is able to synthesise hydrogen cyanide (HCN), a potent inhibitor of cellular respiration. We have recently shown that HCN production by <jats:italic>P. aeruginosa<\/jats:italic> may have a role in CF pathogenesis. This paper describes an investigation of the ability of bacteria of the Bcc to make HCN.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The genome of <jats:italic>Burkholderia cenocepacia<\/jats:italic> has 3 putative HCN synthase encoding (<jats:italic>hcnABC<\/jats:italic>) gene clusters. <jats:italic>B. cenocepacia<\/jats:italic> and all 9 species of the Bcc complex tested were able to make cyanide at comparable levels to <jats:italic>P. aeruginosa<\/jats:italic>, but only when grown surface attached as colonies or during biofilm growth on glass beads. In contrast to <jats:italic>P. aeruginosa<\/jats:italic> and other cyanogenic bacteria, cyanide was not detected during planktonic growth of Bcc strains.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>All species in the Bcc are cyanogenic when grown as surface attached colonies or as biofilms.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-8-108","type":"journal-article","created":{"date-parts":[[2008,6,27]],"date-time":"2008-06-27T18:12:33Z","timestamp":1214590353000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Bacteria of the Burkholderia cepacia complex are cyanogenic under biofilm and colonial growth conditions"],"prefix":"10.1186","volume":"8","author":[{"given":"Ben","family":"Ryall","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James EA","family":"Zlosnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saiko","family":"Hoshino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huw D","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2008,6,27]]},"reference":[{"issue":"9","key":"541_CR1","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1046\/j.1462-2920.2003.00471.x","volume":"5","author":"T Coenye","year":"2003","unstructured":"Coenye T, Vandamme P: Diversity and significance of Burkholderia species occupying diverse ecological niches. 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