{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T21:42:06Z","timestamp":1766266926369},"reference-count":55,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Dengue (DEN) is an infectious disease caused by the DEN virus (DENV), which belongs to the <jats:italic>Flavivirus<\/jats:italic> genus in the family <jats:italic>Flaviviridae<\/jats:italic>. It has a (+) sense RNA genome and is mainly transmitted to humans by the vector mosquito <jats:italic>Aedes aegypti<\/jats:italic>. Dengue fever (DF) and dengue hemorrhagic fever (DHF) are caused by one of four closely related virus serotypes (DENV-1, DENV-2, DENV-3 and DENV-4). Epidemiological and evolutionary studies have indicated that host and viral factors are involved in determining disease outcome and have proved the importance of viral genotype in causing severe epidemics. Host immune status and mosquito vectorial capacity are also important influences on the severity of infection. Therefore, an understanding of the relationship between virus variants with altered amino acids and high pathogenicity will provide more information on the molecular epidemiology of DEN. Accordingly, knowledge of the DENV serotypes and genotypes circulating in the latest DEN outbreaks around the world, including Mexico, will contribute to understanding DEN infections.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>1. We obtained 88 isolates of DENV, 27 from Oaxaca and 61 from Veracruz. 2. Of these 88 isolates, 16 were serotype 1; 62 serotype 2; 7 serotype 3; and 2 serotype 4. One isolate had 2 serotypes (DENV-2 and -1). 3. Partial nucleotide sequences of the genes encoding C- prM (14 sequences), the NS3 helicase domain (7 sequences), the NS5 S-adenosyl methionine transferase domain (7 sequences) and the RNA-dependent RNA polymerase (RdRp) domain (18 sequences) were obtained. Phylogenetic analysis showed that DENV-2 isolates belonged to the Asian\/American genotype. In addition, the Asian\/American genotype was divided into two clusters, one containing the isolates from 2001 and the other the isolates from 2005\u20132006 with high bootstrap support of 94%.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>DENV-2 was the predominant serotype in the DF and DHF outbreak from 2005 to 2006 in Oaxaca State as well as in the 2006 outbreak in Veracruz State, with the Asian\/American genotype prevalent in both states. Interestingly, DENV-1 and DENV-2 were the only serotypes related to DHF cases. In contrast, DENV-3 and DENV-4 were poorly represented according to epidemiological data reported in Mexico. We found that isoleucine was replaced by valine at residue 106 of protein C in the isolates from these 2005\u20132006 outbreaks and in those from the 1997, 1998 and 2001 outbreaks in the Caribbean islands. We suggested that this amino acid change may be used as a signature for isolates arising in the Caribbean islands and pertaining to the Asian\/American genotype. Other amino acid changes are specific for the Asian\/American, Asian and American strains.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-8-117","type":"journal-article","created":{"date-parts":[[2008,7,15]],"date-time":"2008-07-15T06:14:56Z","timestamp":1216102496000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Specific genetic markers for detecting subtypes of dengue virus serotype-2 in isolates from the states of Oaxaca and Veracruz, Mexico"],"prefix":"10.1186","volume":"8","author":[{"given":"Catalina E","family":"Gardella-Garcia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerardo","family":"Perez-Ramirez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel","family":"Navarrete-Espinosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Cisneros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiola","family":"Jimenez-Rojas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis R","family":"Ram\u00edrez-Palacios","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rocio","family":"Rosado-Leon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minerva","family":"Camacho-Nuez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria de L","family":"Munoz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,7,15]]},"reference":[{"issue":"5","key":"550_CR1","doi-asserted-by":"crossref","first-page":"594","DOI":"10.4269\/ajtmh.2002.66.594","volume":"66","author":"F Garcia-Franco","year":"2002","unstructured":"Garcia-Franco F, Munoz ML, Lozano-Fuentes S, Fernandez-Salas I, Garcia-Rejon J, Beaty BJ, Black WC: Large genetic distances among Aedes aegypti populations along the South Pacific coast of Mexico. Am J Trop Med Hyg. 2002, 66 (5): 594-598.","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"550_CR2","doi-asserted-by":"crossref","first-page":"200","DOI":"10.4269\/ajtmh.2000.62.200","volume":"62","author":"N Gorrochotegui-Escalante","year":"2000","unstructured":"Gorrochotegui-Escalante N, Munoz ML, Fernandez-Salas I, Beaty BJ, Black WC: Genetic isolation by distance among Aedes aegypti populations along the northeastern coast of Mexico. Am J Trop Med Hyg. 2000, 62 (2): 200-209.","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"550_CR3","doi-asserted-by":"crossref","first-page":"213","DOI":"10.4269\/ajtmh.2002.66.213","volume":"66","author":"N Gorrochotegui-Escalante","year":"2002","unstructured":"Gorrochotegui-Escalante N, Gomez-Machorro C, Lozano-Fuentes S, Fernandez-Salas L, Munoz ML, Farfan-Ale JA, Garcia-Rejon J, Beaty BJ, Black WC: Breeding structure of Aedes aegypti populations in Mexico varies by region. Am J Trop Med Hyg. 2002, 66 (2): 213-222.","journal-title":"Am J Trop Med Hyg"},{"issue":"3","key":"550_CR4","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1128\/CMR.11.3.480","volume":"11","author":"DJ Gubler","year":"1998","unstructured":"Gubler DJ: Dengue and dengue hemorrhagic fever. Clin Microbiol Rev. 1998, 11 (3): 480-496.","journal-title":"Clin Microbiol Rev"},{"issue":"38","key":"550_CR5","first-page":"362","volume":"81","author":"WHO (World Health Organization)","year":"2006","unstructured":"WHO (World Health Organization): Dengue haemorrhagic fever: early recognition, diagnosis and hospital management \u2013 an audiovisual guide for health care workers responding to outbreaks. Weekly Epidemiological Record. 2006, 81 (38): 362-363.","journal-title":"Weekly Epidemiological Record"},{"key":"550_CR6","unstructured":"Clinical diagnosis. Dengue haemorrhagic fever: diagnosis, treatment, prevention and control. 1997, World Health Organization. Geneva, 12-23. [http:\/\/www.who.int\/csr\/resources\/publications\/dengue\/Denguepublication\/en\/]2"},{"key":"550_CR7","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1590\/S0036-36342005000300002","volume":"47","author":"J Navarrete-Espinosa","year":"2005","unstructured":"Navarrete-Espinosa J, Gomez-Dantes H, Celis-Quintal JG, Vazquez-Martinez JL: Clinical profile of dengue hemorrhagic fever cases in Mexico. Salud Publica Mex. 2005, 47: 193-200.","journal-title":"Salud Publica Mex"},{"key":"550_CR8","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/S0065-3527(03)60010-2","volume":"60","author":"AL Rothman","year":"2003","unstructured":"Rothman AL: Immunology and immunopathogenesis of dengue disease. Adv Virus Res. 2003, 60: 397-419.","journal-title":"Adv Virus Res"},{"issue":"2","key":"550_CR9","doi-asserted-by":"publisher","first-page":"282","DOI":"10.3201\/eid1302.060539","volume":"13","author":"MG Guzman","year":"2007","unstructured":"Guzman MG, Alvarez M, Rodriguez-Roche R, Bernardo L, Montes T, Vazquez S, Morier L, Alvarez A, Gould EA, Kouri G, Halstead SB: Neutralizing antibodies after infection with dengue 1 virus. Emerg Infect Dis. 2007, 13 (2): 282-286.","journal-title":"Emerg Infect Dis"},{"issue":"2","key":"550_CR10","first-page":"89","volume":"36","author":"WK Wang","year":"2003","unstructured":"Wang WK, Chao DY, Lin SR, King CC, Chang SC: Concurrent infections by two dengue virus serotypes among dengue patients in Taiwan. J Microbiol Immunol Infect. 2003, 36 (2): 89-95.","journal-title":"J Microbiol Immunol Infect"},{"issue":"2","key":"550_CR11","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1006\/viro.1997.8504","volume":"230","author":"R Rico-Hesse","year":"1997","unstructured":"Rico-Hesse R, Harrison LM, Salas RA, Tovar D, Nisalak A, Ramos C, Boshell J, de Mesa MT, Nogueira RM, da Rosa AT: Origins of dengue type 2 viruses associated with increased pathogenicity in the Americas. Virology. 1997, 230 (2): 244-251.","journal-title":"Virology"},{"issue":"6","key":"550_CR12","doi-asserted-by":"crossref","first-page":"4738","DOI":"10.1128\/JVI.73.6.4738-4747.1999","volume":"73","author":"KC Leitmeyer","year":"1999","unstructured":"Leitmeyer KC, Vaughn DW, Watts DM, Salas R, Villalobos I, de Chacon, Ramos C, Rico-Hesse R: Dengue virus structural differences that correlate with pathogenesis. J Virol. 1999, 73 (6): 4738-4747.","journal-title":"J Virol"},{"issue":"6","key":"550_CR13","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.4269\/ajtmh.2006.75.1113","volume":"75","author":"M Alvarez","year":"2006","unstructured":"Alvarez M, Rodriguez-Roche R, Bernardo L, Vazquez S, Morier L, Gonzalez D, Castro O, Kouri G, Halstead SB, Guzman MG: Dengue hemorrhagic fever caused by sequential dengue 1\u20133 virus infections over a long time interval: Havana epidemic, 2001\u20132002. Am J Trop Med Hyg. 2006, 75 (6): 1113-1117.","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"550_CR14","doi-asserted-by":"crossref","first-page":"566","DOI":"10.4269\/ajtmh.1997.56.566","volume":"56","author":"S Thein","year":"1997","unstructured":"Thein S, Aung MM, Shwe TN, Aye M, Zaw A, Aye K, Aye KM, Aaskov J: Risk factors in dengue shock syndrome. Am J Trop Med Hyg. 1997, 56 (5): 566-572.","journal-title":"Am J Trop Med Hyg"},{"issue":"3","key":"550_CR15","doi-asserted-by":"crossref","first-page":"241","DOI":"10.4269\/ajtmh.1995.52.241","volume":"52","author":"MG Guzman","year":"1995","unstructured":"Guzman MG, Deubel V, Pelegrino JL, Rosario D, Marrero M, Sariol C, Kouri G: Partial nucleotide and amino acid sequences of the envelope and the envelope\/nonstructural protein-1 gene junction of four dengue-2 virus strains isolated during the 1981 Cuban epidemic. Am J Trop Med Hyg. 1995, 52 (3): 241-246.","journal-title":"Am J Trop Med Hyg"},{"issue":"Pt 12","key":"550_CR16","doi-asserted-by":"publisher","first-page":"2945","DOI":"10.1099\/0022-1317-82-12-2945","volume":"82","author":"NY Uzcategui","year":"2001","unstructured":"Uzcategui NY, Camacho D, Comach G, Cuello de Uzcategui R, Holmes EC, Gould EA: Molecular epidemiology of dengue type 2 virus in Venezuela: evidence for in situ virus evolution and recombination. J Gen Virol. 2001, 82 (Pt 12): 2945-2953.","journal-title":"J Gen Virol"},{"issue":"1","key":"550_CR17","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1007\/s00705-005-0595-9","volume":"151","author":"A Cisneros","year":"2006","unstructured":"Cisneros A, Diaz-Badillo A, Cruz-Martinez G, Tovar R, Ramirez-Palacios LR, Jimenez-Rojas F, Beaty B, Black WC, Munoz LM: Dengue 2 genotypes in the state of Oaxaca, Mexico. Arch Virol. 2006, 151 (1): 113-125.","journal-title":"Arch Virol"},{"issue":"9188","key":"550_CR18","doi-asserted-by":"publisher","first-page":"1431","DOI":"10.1016\/S0140-6736(99)04015-5","volume":"354","author":"DM Watts","year":"1999","unstructured":"Watts DM, Porter KR, Putvatana P, Vasquez B, Calampa C, Hayes CG, Halstead SB: Failure of secondary infection with American genotype dengue 2 to cause dengue haemorrhagic fever. Lancet. 1999, 354 (9188): 1431-1434.","journal-title":"Lancet"},{"issue":"Pt 1","key":"550_CR19","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1099\/0022-1317-75-1-65","volume":"75","author":"RS Lanciotti","year":"1994","unstructured":"Lanciotti RS, Lewis JG, Gubler DJ, Trent DW: Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol. 1994, 75 (Pt 1): 65-75.","journal-title":"J Gen Virol"},{"issue":"Pt 9","key":"550_CR20","doi-asserted-by":"publisher","first-page":"2279","DOI":"10.1099\/0022-1317-78-9-2279","volume":"78","author":"RS Lanciotti","year":"1997","unstructured":"Lanciotti RS, Gubler DJ, Trent DW: Molecular evolution and phylogeny of dengue-4 viruses. J Gen Virol. 1997, 78 (Pt 9): 2279-2284.","journal-title":"J Gen Virol"},{"issue":"1","key":"550_CR21","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1006\/viro.1993.1582","volume":"197","author":"JA Lewis","year":"1993","unstructured":"Lewis JA, Chang GJ, Lanciotti RS, Kinney RM, Mayer LW, Trent DW: Phylogenetic relationships of dengue-2 viruses. Virology. 1993, 197 (1): 216-224.","journal-title":"Virology"},{"issue":"2","key":"550_CR22","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1016\/0042-6822(90)90102-W","volume":"174","author":"R Rico-Hesse","year":"1990","unstructured":"Rico-Hesse R: Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology. 1990, 174 (2): 479-493.","journal-title":"Virology"},{"issue":"1","key":"550_CR23","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1006\/viro.2002.1447","volume":"298","author":"SS Twiddy","year":"2002","unstructured":"Twiddy SS, Farrar JJ, Vinh Chau N, Wills B, Gould EA, Gritsun T, Lloyd G, Holmes EC: Phylogenetic relationships and differential selection pressures among genotypes of dengue-2 virus. Virology. 2002, 298 (1): 63-72.","journal-title":"Virology"},{"issue":"1","key":"550_CR24","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/S1567-1348(03)00004-2","volume":"3","author":"EC Holmes","year":"2003","unstructured":"Holmes EC, Twiddy SS: The origin, emergence and evolutionary genetics of dengue virus. Infect Genet Evol. 2003, 3 (1): 19-28.","journal-title":"Infect Genet Evol"},{"issue":"Pt 4","key":"550_CR25","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1099\/vir.0.81486-0","volume":"87","author":"C Zhang","year":"2006","unstructured":"Zhang C, Mammen MP, Chinnawirotpisan P, Klungthong C, Rodpradit P, Nisalak A, Vaughn DW, Nimmannitya S, Kalayanarooj S, Holmes EC: Structure and age of genetic diversity of dengue virus type 2 in Thailand. J Gen Virol. 2006, 87 (Pt 4): 873-883.","journal-title":"J Gen Virol"},{"issue":"11","key":"550_CR26","first-page":"940","volume":"47","author":"SK Chua","year":"2006","unstructured":"Chua SK, Selvanesan S, Sivalingam B, Chem YK, Norizah I, Zuridah H, Kumarasamy V, Chua KB: Isolation of monoclonal antibodies-escape variant of dengue virus serotype 1. Singapore Med J. 2006, 47 (11): 940-946.","journal-title":"Singapore Med J"},{"issue":"3","key":"550_CR27","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1007\/s00705-004-0445-1","volume":"150","author":"R Rodriguez-Roche","year":"2005","unstructured":"Rodriguez-Roche R, Alvarez M, Gritsun T, Rosario D, Halstead S, Kouri G, Gould EA, Guzman MG: Dengue virus type 2 in Cuba, 1997: conservation of E gene sequence in isolates obtained at different times during the epidemic. Arch Virol. 2005, 150 (3): 415-425.","journal-title":"Arch Virol"},{"issue":"14","key":"550_CR28","doi-asserted-by":"publisher","first-page":"10678","DOI":"10.1074\/jbc.M607273200","volume":"282","author":"H Malet","year":"2007","unstructured":"Malet H, Egloff MP, Selisko B, Butcher RE, Wright PJ, Roberts M, Gruez A, Sulzenbacher G, Vonrhein C, Bricogne G, Mackenzie JM, Khromykh AA, Davidson AD, Canard B: Crystal structure of the RNA polymerase domain of the West Nile virus non-structural protein 5. J Biol Chem. 2007, 282 (14): 10678-10689.","journal-title":"J Biol Chem"},{"issue":"1","key":"550_CR29","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/JVI.72.1.73-83.1998","volume":"72","author":"G Kuno","year":"1998","unstructured":"Kuno G, Chang GJ, Tsuchiya KR, Karabatsos N, Cropp CB: Phylogeny of the genus Flavivirus. J Virol. 1998, 72 (1): 73-83.","journal-title":"J Virol"},{"issue":"3","key":"550_CR30","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1590\/S0074-02762003000300015","volume":"98","author":"FG Baleotti","year":"2003","unstructured":"Baleotti FG, Moreli ML, Figueiredo LT: Brazilian Flavivirus phylogeny based on NS5. Mem Inst Oswaldo Cruz. 2003, 98 (3): 379-382.","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"550_CR31","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1128\/JVI.01788-07","volume":"82","author":"D Luo","year":"2008","unstructured":"Luo D, Xu T, Hunke C, Gr\u00fcber G, Vasudevan SG, Lescar J: Crystal structure of the NS3 protease-helicase from dengue virus. J Virol. 2008, 82 (1): 173-183.","journal-title":"J Virol"},{"issue":"5","key":"550_CR32","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1016\/j.jmgm.2006.04.001","volume":"25","author":"L Rosales-Leon","year":"2007","unstructured":"Rosales-Leon L, Ortega-Lule G, Ruiz-Ordaz B: Analysis of the domain interactions between the protease and helicase of NS3 in dengue and hepatitis C virus. J Mol Graph Model. 2007, 25 (5): 585-594.","journal-title":"J Mol Graph Model"},{"key":"550_CR33","first-page":"28","volume":"28","author":"A Cisneros-Solano","year":"2004","unstructured":"Cisneros-Solano A, Moreno-Altamirano MM, Martinez-Soriano U, Jimenez-Rojas F, Diaz-Badillo A, Munoz ML: Sero-epidemiological and virological investigation of dengue infection in Oaxaca, Mexico, during 2000\u20132001. Dengue Bulletin. 2004, 28: 28-34.","journal-title":"Dengue Bulletin"},{"key":"550_CR34","unstructured":"DGEPI (Direcci\u00f3n General de Epidemiologia de la Secretaria de Salud) 2001\u20132006. Boletin Epidemiolgia. [http:\/\/www.dgepi.salud.gob.mx\/boletin\/]"},{"issue":"5","key":"550_CR35","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1159\/000073208","volume":"46","author":"WJ Chen","year":"2003","unstructured":"Chen WJ, Wu HR, Chiou SS: E\/NS1 modifications of dengue 2 virus after serial passages in mammalian and\/or mosquito cells. Intervirology. 2003, 46 (5): 289-295.","journal-title":"Intervirology"},{"issue":"2","key":"550_CR36","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1006\/viro.1997.8570","volume":"232","author":"E Lee","year":"1997","unstructured":"Lee E, Weir RC, Dalgarno L: Changes in the dengue virus major envelope protein on passaging and their localization on the three-dimensional structure of the protein. Virology. 1997, 232 (2): 281-290.","journal-title":"Virology"},{"issue":"Pt 7","key":"550_CR37","doi-asserted-by":"publisher","first-page":"1679","DOI":"10.1099\/0022-1317-83-7-1679","volume":"83","author":"SS Twiddy","year":"2002","unstructured":"Twiddy SS, Woelk CH, Holmes EC: Phylogenetic evidence for adaptive evolution of dengue viruses in nature. J Gen Virol. 2002, 83 (Pt 7): 1679-1689.","journal-title":"J Gen Virol"},{"issue":"3","key":"550_CR38","doi-asserted-by":"crossref","first-page":"364","DOI":"10.4269\/ajtmh.2008.78.364","volume":"78","author":"MM Ramos","year":"2008","unstructured":"Ramos MM, Mohammed H, Zielinski-Gutierrez E, Hayden MH, Lopez JL, Fournier M, Trujillo AR, Burton R, Brunkard JM, Anaya-Lopez L, Banicki AA, Morales PK, Smith B, Munoz JL, Waterman SH, Dengue Serosurvey Working Group: Epidemic dengue and dengue hemorrhagic fever at the Texas-Mexico border: Results of a household-based seroepidemiologic survey, December 2005. Am J Trop Med Hyg. 2008, 78 (3): 364-369.","journal-title":"Am J Trop Med Hyg"},{"key":"550_CR39","unstructured":"Manual para la Vigilancia, Diagnostico, Prevencion y Control del Dengue. [http:\/\/salud.tamaulipas.gob.mx\/epidemiologia\/pdf's\/MANUAL-DENGUE%202006.pdf]"},{"issue":"6","key":"550_CR40","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1590\/S0100-879X2005000600005","volume":"38","author":"RJ Pires Neto","year":"2005","unstructured":"Pires Neto RJ, Lima DM, de Paula SO, Lima CM, Rocco IM, Fonseca BA: Molecular epidemiology of type 1 and 2 dengue viruses in Brazil from 1988 to 2001. Braz J Med Biol Res. 2005, 38 (6): 843-852.","journal-title":"Braz J Med Biol Res"},{"issue":"9","key":"550_CR41","doi-asserted-by":"publisher","first-page":"4753","DOI":"10.1128\/JVI.02283-06","volume":"81","author":"TL Yap","year":"2007","unstructured":"Yap TL, Xu T, Chen YL, Malet H, Egloff MP, Canard B, Vasudevan SG, Lescar J: Crystal structure of the dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-angstrom resolution. J Virol. 2007, 81 (9): 4753-4765.","journal-title":"J Virol"},{"issue":"1","key":"550_CR42","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1006\/viro.2002.1549","volume":"301","author":"V Wittke","year":"2002","unstructured":"Wittke V, Robb TE, Thu HM, Nisalak A, Nimmannitya S, Kalayanrooj S, Vaughn DW, Endy TP, Holmes EC, Aaskov JG: Extinction and rapid emergence of strains of dengue 3 virus during an interepidemic period. Virology. 2002, 301 (1): 148-156.","journal-title":"Virology"},{"issue":"24","key":"550_CR43","doi-asserted-by":"publisher","first-page":"15123","DOI":"10.1128\/JVI.79.24.15123-15130.2005","volume":"79","author":"C Zhang","year":"2005","unstructured":"Zhang C, Mammen MP, Chinnawirotpisan P, Klungthong C, Rodpradit P, Monkongdee P, Nimmannitya S, Kalayanarooj S, Holmes EC: Clade replacements in dengue virus serotypes 1 and 3 are associated with changing serotype prevalence. J Virol. 2005, 79 (24): 15123-15130.","journal-title":"J Virol"},{"issue":"2","key":"550_CR44","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.virol.2005.01.037","volume":"334","author":"R Rodriguez-Roche","year":"2005","unstructured":"Rodriguez-Roche R, Alvarez M, Gritsun T, Halstead S, Kouri G, Gould EA, Guzman MG: Virus evolution during a severe dengue epidemic in Cuba, 1997. Virology. 2005, 334 (2): 154-159.","journal-title":"Virology"},{"issue":"5","key":"550_CR45","doi-asserted-by":"crossref","first-page":"539","DOI":"10.4269\/ajtmh.2003.68.539","volume":"68","author":"PM Armstrong","year":"2003","unstructured":"Armstrong PM, Rico-Hesse R: Efficiency of dengue serotype 2 virus strains to infect and disseminate in Aedes aegypti. Am J Trop Med Hyg. 2003, 68 (5): 539-544.","journal-title":"Am J Trop Med Hyg"},{"key":"550_CR46","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1186\/1471-2180-6-85","volume":"6","author":"RF Mercado-Curiel","year":"2006","unstructured":"Mercado-Curiel RF, Esquinca-Aviles HA, Tovar R, Diaz-Badillo A, Camacho-Nuez M, Munoz ML: The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells. BMC Microbiol. 2006, 6: 85-","journal-title":"BMC Microbiol"},{"issue":"2","key":"550_CR47","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/S0378-1097(98)00434-0","volume":"168","author":"ML Munoz","year":"1998","unstructured":"Munoz ML, Cisneros A, Cruz J, Das P, Tovar R, Ortega A: Putative dengue virus receptors from mosquito cells. FEMS Microbiol Lett. 1998, 168 (2): 251-258.","journal-title":"FEMS Microbiol Lett"},{"key":"550_CR48","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1186\/1471-2180-8-28","volume":"8","author":"KM Pepin","year":"2008","unstructured":"Pepin KM, Lambeth K, Hanley KA: Asymmetric competitive suppression between strains of dengue virus. BMC Microbiol. 2008, 8: 28-","journal-title":"BMC Microbiol"},{"issue":"9","key":"550_CR49","doi-asserted-by":"publisher","first-page":"4171","DOI":"10.1073\/pnas.90.9.4171","volume":"90","author":"JW Drake","year":"1993","unstructured":"Drake JW: Rates of spontaneous mutation among RNA viruses. Proc Natl Acad Sci USA. 1993, 90 (9): 4171-4175.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"13","key":"550_CR50","doi-asserted-by":"publisher","first-page":"7352","DOI":"10.1073\/pnas.96.13.7352","volume":"96","author":"M Worobey","year":"1999","unstructured":"Worobey M, Rambaut A, Holmes EC: Widespread intra-serotype recombination in natural populations of dengue virus. Proc Natl Acad Sci USA. 1999, 96 (13): 7352-7357.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"10","key":"550_CR51","doi-asserted-by":"publisher","first-page":"1650","DOI":"10.1093\/molbev\/msg182","volume":"20","author":"SN Bennett","year":"2003","unstructured":"Bennett SN, Holmes EC, Chirivella M, Rodriguez DM, Beltran M, Vorndam V, Gubler DJ, McMillan WO: Selection-driven evolution of emergent dengue virus. Mol Biol Evol. 2003, 20 (10): 1650-1658.","journal-title":"Mol Biol Evol"},{"issue":"2\u20133","key":"550_CR52","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/0166-0934(94)00104-O","volume":"51","author":"CL Seah","year":"1995","unstructured":"Seah CL, Chow VT, Tan HC, Can YC: Rapid, single-step RT-PCR typing of dengue viruses using five NS3 gene primers. J Virol Methods. 1995, 51 (2\u20133): 193-200.","journal-title":"J Virol Methods"},{"issue":"4","key":"550_CR53","first-page":"406","volume":"4","author":"N Saitou","year":"1987","unstructured":"Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987, 4 (4): 406-425.","journal-title":"Mol Biol Evol"},{"issue":"3","key":"550_CR54","first-page":"512","volume":"10","author":"K Tamura","year":"1993","unstructured":"Tamura K, Nei M: Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993, 10 (3): 512-526.","journal-title":"Mol Biol Evol"},{"issue":"8","key":"550_CR55","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.1093\/molbev\/msm092","volume":"24","author":"K Tamura","year":"2007","unstructured":"Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24 (8): 1596-1599.","journal-title":"Mol Biol Evol"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2180-8-117.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T10:10:09Z","timestamp":1630491009000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/1471-2180-8-117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,15]]},"references-count":55,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["550"],"URL":"https:\/\/doi.org\/10.1186\/1471-2180-8-117","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,15]]},"assertion":[{"value":"22 December 2007","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 July 2008","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 July 2008","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"117"}}