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Herein we describe, for the first time, the development of a non-invasive luciferase-based reporter system for real-time tracking of <jats:italic>Bifidobacterium<\/jats:italic> species <jats:italic>in vivo<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The reporter vector pLuxMC1 is based on the recently described theta-type plasmid pBC1 from <jats:italic>B. catenatulatum<\/jats:italic> [1] and the <jats:italic>luxABCDE<\/jats:italic> operon from pPL2lux [2]. Derivatives of pLuxMC1, harbouring a bifidobacterial promoter (pLuxMC2) as well as a synthetically derived promoter (pLuxMC3) [3] placed upstream of <jats:italic>luxABCDE<\/jats:italic>, were constructed and found to stably replicate in <jats:italic>B. breve<\/jats:italic> UCC2003. The subsequent analysis of these strains allowed us to assess the functionality of pLuxMC1 both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Our results demonstrate the potential of pLuxMC1 as a real-time, non-invasive reporter system for <jats:italic>Bifidobacterium<\/jats:italic>. It has also allowed us, for the first time, to track the colonisation potential and persistence of this probiotic species in real time. An interesting and significant outcome of the study is the identification of the caecum as a niche environment for <jats:italic>B. breve<\/jats:italic> UCC2003 within the mouse gastrointestinal tract (GI) tract.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-8-161","type":"journal-article","created":{"date-parts":[[2008,9,24]],"date-time":"2008-09-24T18:13:37Z","timestamp":1222280017000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice"],"prefix":"10.1186","volume":"8","author":[{"given":"Michelle","family":"Cronin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy D","family":"Sleator","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin","family":"Hill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald F","family":"Fitzgerald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douwe","family":"van Sinderen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,9,24]]},"reference":[{"key":"594_CR1","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.plasmid.2006.07.004","volume":"57","author":"P Alvarez-Martin","year":"2007","unstructured":"Alvarez-Martin P, Florez AB, Mayo B: Screening for plasmids among human bifidobacteria species: Sequencing and analysis of pBC1 from Bifidobacterium catenulatum L48. 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