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In the present study, we investigated mechanism of<jats:italic>B. abortus<\/jats:italic>invasion into TG cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed internalization and intracellular growth of<jats:italic>B. abortus<\/jats:italic>in cultured TG cells. A monoclonal antibody that inhibits bacterial internalization was isolated and this reacted with heat shock cognate protein 70 (Hsc70). Depletion and over expression of Hsc70 in TG cells inhibited and promoted bacterial internalization, respectively. IFN-\u03b3 receptor was expressed in TG cells and IFN-\u03b3 treatment enhanced the uptake of bacteria by TG cells. Administering the anti-Hsc70 antibody to pregnant mice served to prevent infectious abortion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>B. abortus<\/jats:italic>infection of TG cells in placenta is mediated by Hsc70, and that such infection leads to infectious abortion.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2180-8-212","type":"journal-article","created":{"date-parts":[[2008,12,5]],"date-time":"2008-12-05T07:12:38Z","timestamp":1228461158000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Heat shock cognate protein 70 contributes to Brucella invasion into trophoblast giant cells that cause infectious abortion"],"prefix":"10.1186","volume":"8","author":[{"given":"Kenta","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Tachibana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidefumi","family":"Furuoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motohiro","family":"Horiuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahisa","family":"Watarai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,12,5]]},"reference":[{"key":"645_CR1","doi-asserted-by":"publisher","first-page":"648","DOI":"10.1016\/j.theriogenology.2006.04.021","volume":"66","author":"MD Givens","year":"2006","unstructured":"Givens MD: A clinical, evidence-based approach to infectious causes of infertility in beef cattle. 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