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The mutant strain exhibited a 4-log decrease in infectivity, relative to the wild-type strain, when needle inoculated into mice. Upon complementation of the DbpBA-mutant strain with DbpA, the wild-type level of infectivity was restored. In addition, we demonstrated that the DbpBA-deficient mutant was able to colonize<jats:italic>Ixodes scapularis<\/jats:italic>larval ticks after feeding on infected mice and persist within the ticks during the molt to the nymphal state. Moreover, surprisingly, the DbpBA-mutant strain was capable of being transmitted to na\u00efve mice via tick bite, giving rise to infected mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that DbpBA is not required for the natural tick-transmission process to mammals, despite inferences from needle-inoculation experiments implying a requirement for DbpBA during mammalian infection. The combined findings also send a cautionary note regarding how results from needle-inoculation experiments with mice should be interpreted.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2180-8-82","type":"journal-article","created":{"date-parts":[[2008,5,28]],"date-time":"2008-05-28T18:14:31Z","timestamp":1211998471000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["Assessment of decorin-binding protein A to the infectivity of Borrelia burgdorferi in the murine models of needle and tick infection"],"prefix":"10.1186","volume":"8","author":[{"given":"Jon S","family":"Blevins","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayla E","family":"Hagman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael V","family":"Norgard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2008,5,28]]},"reference":[{"issue":"8","key":"515_CR1","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1172\/JCI21681","volume":"113","author":"AC Steere","year":"2004","unstructured":"Steere AC, Coburn J, Glickstein L: The emergence of Lyme disease. 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