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FliY is a flagellar motor switch protein that controls flagellar motor direction in other microbes, but its role in <jats:italic>Leptospira<\/jats:italic>, and paricularly in pathogenicity remains unknown.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>A suicide plasmid for the <jats:italic>fliY<\/jats:italic> gene of <jats:italic>Leptospira interrogans<\/jats:italic> serogroup Icterohaemorrhagiae serovar Lai strain Lai that was disrupted by inserting the ampicillin resistance gene (<jats:italic>bla<\/jats:italic>) was constructed, and the inactivation of <jats:italic>fliY<\/jats:italic> gene in a mutant (<jats:italic>fliY<\/jats:italic>\n              <jats:sup>-<\/jats:sup>) was confirmed by PCR and Western Blot analysis. The inactivation resulted in the mRNA absence of <jats:italic>fliP<\/jats:italic> and <jats:italic>fliQ<\/jats:italic> genes which are located downstream of the <jats:italic>fliY<\/jats:italic> gene in the same operon. The mutant displayed visibly weakened rotative motion in liquid medium and its migration on semisolid medium was also markedly attenuated compared to the wild-type strain. Compared to the wild-type strain, the mutant showed much lower levels of adhesion to murine macrophages and apoptosis-inducing ability, and its lethality to guinea pigs was also significantly decreased.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Inactivation of <jats:italic>fliY<\/jats:italic>, by the method used in this paper, clearly had polar effects on downstream genes. The phentotypes observed, including lower pathogenicity, could be a consequence of <jats:italic>fliY<\/jats:italic> inactivation, but also a consequence of the polar effects.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-9-253","type":"journal-article","created":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T12:50:37Z","timestamp":1260449437000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Inactivation of the fliY gene encoding a flagellar motor switch protein attenuates mobility and virulence of Leptospira interrogansstrain Lai"],"prefix":"10.1186","volume":"9","author":[{"given":"Sumei","family":"Liao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M","family":"Ojcius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senlin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2009,12,9]]},"reference":[{"key":"924_CR1","volume-title":"Leptospira and leptospirosis","author":"S Faine","year":"1999","unstructured":"Faine S, Adher B, Bloin C, Perolat P: Leptospira and leptospirosis. 1999, Australia: MedSci, 2","edition":"2"},{"key":"924_CR2","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","volume":"3","author":"AR Bharti","year":"2003","unstructured":"Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM: Leptospirosis: a zoonotic disease of global importance. 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