{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T19:49:59Z","timestamp":1785440999604,"version":"3.56.0"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>The <jats:italic>Salmonella<\/jats:italic> PreA\/PreB two-component system (TCS) is an ortholog of the QseBC TCS of <jats:italic>Escherichia coli<\/jats:italic>. In both <jats:italic>Salmonella<\/jats:italic> and <jats:italic>E. coli<\/jats:italic>, this system has been shown to affect motility and virulence in response to quorum-sensing and hormonal signals, and to affect the transcription of the <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium (<jats:italic>S<\/jats:italic>. Typhimurium) <jats:italic>pmrAB<\/jats:italic> operon, which encodes an important virulence-associated TCS.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>To determine the PreA\/PreB regulon in <jats:italic>S<\/jats:italic>. Typhimurium, we performed DNA microarrays comparing the wild type strain and various <jats:italic>preA<\/jats:italic> and\/or <jats:italic>preB<\/jats:italic> mutants in the presence of ectopically expressed <jats:italic>preA<\/jats:italic> (<jats:italic>qseB<\/jats:italic>). These data confirmed our previous findings of the negative effect of PreB on PreA gene regulation and identified candidate PreA-regulated genes. A proportion of the activated loci were previously identified as PmrA-activated genes (<jats:italic>yibD<\/jats:italic>, <jats:italic>pmrAB<\/jats:italic>, <jats:italic>cptA<\/jats:italic>, etc.) or were genes located in the local region around <jats:italic>preA<\/jats:italic>, including the <jats:italic>preAB<\/jats:italic> operon. The transcriptional units were defined in this local region by RT-PCR, suggesting three PreA activated operons composed of <jats:italic>preA-preB<\/jats:italic>, <jats:italic>mdaB-ygiN<\/jats:italic>, and <jats:italic>ygiW<\/jats:italic>-STM3175. Several putative virulence-related phenotypes were examined for <jats:italic>preAB<\/jats:italic> mutants, resulting in the observation of a host cell invasion and slight virulence defect of a <jats:italic>preAB<\/jats:italic> mutant. Contrary to previous reports on this TCS, we were unable to show a PreA\/PreB-dependent effect of the quorum-sensing signal AI-2 or of epinephrine on <jats:italic>S<\/jats:italic>. Typhimurium with regard to bacterial motility.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This work further characterizes this unorthadox OmpR\/EnvZ class TCS and provides novel candidate regulated genes for further study. This first in-depth study of the PreA\/PreB regulatory system phenotypes and regulation suggests significant comparative differences to the reported function of the orthologous QseB\/QseC in <jats:italic>E. coli<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2180-9-42","type":"journal-article","created":{"date-parts":[[2009,2,24]],"date-time":"2009-02-24T07:24:04Z","timestamp":1235460244000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Genome-wide analysis of the PreA\/PreB (QseB\/QseC) regulon of Salmonella enterica serovar Typhimurium"],"prefix":"10.1186","volume":"9","author":[{"given":"Massimo","family":"Merighi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alecia N","family":"Septer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Carroll-Portillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditi","family":"Bhatiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Porwollik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"McClelland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John S","family":"Gunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2009,2,23]]},"reference":[{"key":"713_CR1","first-page":"5","volume":"48","author":"MV Stoycheva","year":"2006","unstructured":"Stoycheva MV, Murdjeva MA: Antimicrobial therapy of salmonelloses \u2013 current state and perspectives. 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