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The current lack of an approved tetravalent vaccine and ineffective insecticide control measures warrant a search for alternatives to effectively combat DENV. The\n                      <jats:italic>trans<\/jats:italic>\n                      -splicing variant of the\n                      <jats:italic>Tetrahymena thermophila<\/jats:italic>\n                      group I intron catalytic RNA, or ribozyme, is a powerful tool for post-transcriptional RNA modification. The nature of the ribozyme and the predictability with which it can be directed makes it a powerful tool for modifying RNA in nearly any cell type without the need for genome-altering gene therapy techniques or dependence on native cofactors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Several anti-DENV Group I\n                      <jats:italic>trans<\/jats:italic>\n                      -splicing introns (\u03b1DENV-GrpIs) were designed and tested for their ability to target DENV-2 NGC genomes\n                      <jats:italic>in situ<\/jats:italic>\n                      . We have successfully targeted two different uracil bases on the positive sense genomic strand within the highly conserved 5'-3' cyclization sequence (CS) region common to all serotypes of DENV with our \u03b1DENV-GrpIs. Our ribozymes have demonstrated ability to specifically\n                      <jats:italic>trans<\/jats:italic>\n                      -splice a new RNA sequence downstream of the targeted site\n                      <jats:italic>in vitro<\/jats:italic>\n                      and in transfected insect cells as analyzed by firefly luciferase and RT-PCR assays. The effectiveness of these \u03b1DENV-GrpIs to target infecting DENV genomes is also validated in transfected or transformed Aedes mosquito cell lines upon infection with unattenuated DENV-2 NGC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Analysis shows that our \u03b1DENV-GrpIs have the ability to effectively\n                      <jats:italic>trans<\/jats:italic>\n                      -splice the DENV genome\n                      <jats:italic>in situ<\/jats:italic>\n                      . Notably, these results show that the \u03b1DENV-GrpI 9v1, designed to be active against all forms of Dengue virus, effectively targeted the DENV-2 NGC genome in a sequence specific manner. These novel \u03b1DENV-GrpI introns provide a striking alternative to other RNA based approaches for the transgenic suppression of DENV in transformed mosquito cells and tissues.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/1471-2199-11-84","type":"journal-article","created":{"date-parts":[[2010,11,16]],"date-time":"2010-11-16T02:16:12Z","timestamp":1289873772000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans-splicing group I introns"],"prefix":"10.1186","volume":"11","author":[{"given":"James R","family":"Carter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H","family":"Keith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradip V","family":"Barde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tresa S","family":"Fraser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Malcolm J","family":"Fraser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,11,15]]},"reference":[{"issue":"23","key":"570_CR1","doi-asserted-by":"publisher","first-page":"11418","DOI":"10.1128\/JVI.01257-06","volume":"80","author":"K Clyde","year":"2006","unstructured":"Clyde K, Kyle JL, Harris E: Recent advances in deciphering viral and host determinants of dengue virus replication and pathogenesis. 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