{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:29:01Z","timestamp":1764700141016},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Molecular Biol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Loss of heterozygosity (LOH) contributes to many cancers, but the rate at which these events occur in normal cells of the body is not clear. LOH would be detectable in diverse cell types in the body if this event were to confer an obvious cellular phenotype. Mice that carry two different fluorescent protein genes as alleles of a locus would seem to be a useful tool for addressing this issue because LOH would change a cell's phenotype from dichromatic to monochromatic. In addition, LOH caused by mitotic crossing over might be discernable in tissues because this event produces a pair of neighboring monochromatic cells that are different colors.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>As a step in assessing the utility of this approach, we derived primary embryonic fibroblast populations and embryonic stem cell lines from mice that carried two different fluorescent protein genes as alleles at the chromosome 6 locus, ROSA26. Fluorescence activated cell sorting (FACS) showed that the vast majority of cells in each line expressed the two marker proteins at similar levels, and that populations exhibited expression noise similar to that seen in bacteria and yeast. Cells with a monochromatic phenotype were present at frequencies on the order of 10<jats:sup>-4<\/jats:sup> and appeared to be produced at a rate of approximately 10<jats:sup>-5<\/jats:sup> variant cells per mitosis. 45 of 45 stably monochromatic ES cell clones exhibited loss of the expected allele at the ROSA26 locus. More than half of these clones retained heterozygosity at a locus between ROSA26 and the centromere. Other clones exhibited LOH near the centromere, but were disomic for chromosome 6.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Allelic fluorescent markers allowed LOH at the ROSA26 locus to be detected by FACS. LOH at this locus was usually not accompanied by LOH near the centromere, suggesting that mitotic recombination was the major cause of ROSA26 LOH. Dichromatic mouse embryonic cells provide a novel system for studying genetic\/karyotypic stability and factors influencing expression from allelic genes. Similar approaches will allow these phenomena to be studied in tissues.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2199-7-36","type":"journal-article","created":{"date-parts":[[2006,10,17]],"date-time":"2006-10-17T12:25:05Z","timestamp":1161087905000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Expression and loss of alleles in cultured mouse embryonic fibroblasts and stem cells carrying allelic fluorescent protein genes"],"prefix":"10.1186","volume":"7","author":[{"given":"Jon S","family":"Larson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moying","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jared M","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saundra L","family":"Stringer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James R","family":"Stringer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2006,10,16]]},"reference":[{"key":"125_CR1","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1038\/329645a0","volume":"329","author":"HJ Scrable","year":"1987","unstructured":"Scrable HJ, Witte DP, Lampkin BC, Cavenee WK: Chromosomal localization of the human rhabdomyosarcoma locus by mitotic recombination mapping. Nature. 1987, 329: 645-647. 10.1038\/329645a0","journal-title":"Nature"},{"key":"125_CR2","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1038\/305779a0","volume":"305","author":"WK Cavenee","year":"1983","unstructured":"Cavenee WK, Dryja TP, Phillips RA, Benedict WF, Godbout R, Gallie BL, Murphree AL, Strong LC, White RL: Expression of recessive alleles by chromosomal mechanisms in retinoblastoma. Nature. 1983, 305: 779-784. 10.1038\/305779a0","journal-title":"Nature"},{"key":"125_CR3","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1002\/gcc.10253","volume":"38","author":"WK Cavenee","year":"2003","unstructured":"Cavenee WK: The recessive nature of dominance. Genes Chromosomes Cancer. 2003, 38: 322-325. 10.1002\/gcc.10253","journal-title":"Genes Chromosomes Cancer"},{"key":"125_CR4","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1002\/ajmg.10320","volume":"111","author":"AG Knudson","year":"2002","unstructured":"Knudson AG: Cancer genetics. Am J Med Genet. 2002, 111: 96-102. 10.1002\/ajmg.10320","journal-title":"Am J Med Genet"},{"key":"125_CR5","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1038\/85808","volume":"27","author":"L Lefebvre","year":"2001","unstructured":"Lefebvre L, Dionne N, Karaskova J, Squire JA, Nagy A: Selection for transgene homozygosity in embryonic stem cells results in extensive loss of heterozygosity. Nat Genet. 2001, 27: 257-258. 10.1038\/85808","journal-title":"Nat Genet"},{"key":"125_CR6","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1097\/00001622-200201000-00012","volume":"14","author":"S Thiagalingam","year":"2002","unstructured":"Thiagalingam S, Foy RL, Cheng KH, Lee HJ, Thiagalingam A, Ponte JF: Loss of heterozygosity as a predictor to map tumor suppressor genes in cancer: molecular basis of its occurrence. Curr Opin Oncol. 2002, 14: 65-72. 10.1097\/00001622-200201000-00012","journal-title":"Curr Opin Oncol"},{"key":"125_CR7","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1002\/ajmg.1320060207","volume":"6","author":"E Engel","year":"1980","unstructured":"Engel E: A new genetic concept: uniparental disomy and its potential effect, isodisomy. Am J Med Genet. 1980, 6: 137-143. 10.1002\/ajmg.1320060207","journal-title":"Am J Med Genet"},{"key":"125_CR8","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1007\/s00439-005-1295-y","volume":"117","author":"R Pop","year":"2005","unstructured":"Pop R, Zaragoza MV, Gaudette M, Dohrmann U, Scherer G: A homozygous nonsense mutation in SOX9 in the dominant disorder campomelic dysplasia: a case of mitotic gene conversion. Hum Genet. 2005, 117: 43-53. 10.1007\/s00439-005-1295-y","journal-title":"Hum Genet"},{"key":"125_CR9","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1146\/annurev.ge.25.120191.001433","volume":"25","author":"D Lasko","year":"1991","unstructured":"Lasko D, Cavenee W, Nordenskjold M: Loss of constitutional heterozygosity in human cancer. Annu Rev Genet. 1991, 25: 281-314. 10.1146\/annurev.ge.25.120191.001433","journal-title":"Annu Rev Genet"},{"key":"125_CR10","doi-asserted-by":"crossref","first-page":"5599","DOI":"10.1523\/JNEUROSCI.23-13-05599.2003","volume":"23","author":"D Kaushal","year":"2003","unstructured":"Kaushal D, Contos JJ, Treuner K, Yang AH, Kingsbury MA, Rehen SK, McConnell MJ, Okabe M, Barlow C, Chun J: Alteration of gene expression by chromosome loss in the postnatal mouse brain. J Neurosci. 2003, 23: 5599-5606.","journal-title":"J Neurosci"},{"key":"125_CR11","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1073\/pnas.22.7.451","volume":"22","author":"C Stern","year":"1936","unstructured":"Stern C, Rentschler V: The Effect of Temperature on the Frequency of Somatic Crossing-Over in Drosophila Melanogaster. Proc Natl Acad Sci U S A. 1936, 22: 451-453. 10.1073\/pnas.22.7.451","journal-title":"Proc Natl Acad Sci U S A"},{"key":"125_CR12","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1471-213X-1-4","volume":"1","author":"S Srinivas","year":"2001","unstructured":"Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini F: Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol. 2001, 1: 4- 10.1186\/1471-213X-1-4","journal-title":"BMC Dev Biol"},{"key":"125_CR13","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1002\/(SICI)1097-0177(199705)209:1<85::AID-AJA8>3.0.CO;2-T","volume":"209","author":"X Liu","year":"1997","unstructured":"Liu X, Wu H, Loring J, Hormuzdi S, Disteche CM, Bornstein P, Jaenisch R: Trisomy eight in ES cells is a common potential problem in gene targeting and interferes with germ line transmission. Dev Dyn. 1997, 209: 85-91. 10.1002\/(SICI)1097-0177(199705)209:1<85::AID-AJA8>3.0.CO;2-T","journal-title":"Dev Dyn"},{"key":"125_CR14","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1023\/A:1018418914106","volume":"6","author":"L Longo","year":"1997","unstructured":"Longo L, Bygrave A, Grosveld FG, Pandolfi PP: The chromosome make-up of mouse embryonic stem cells is predictive of somatic and germ cell chimaerism. Transgenic Res. 1997, 6: 321-328. 10.1023\/A:1018418914106","journal-title":"Transgenic Res"},{"key":"125_CR15","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.dnarep.2005.09.009","volume":"5","author":"EC Friedberg","year":"2006","unstructured":"Friedberg EC, Meira LB: Database of mouse strains carrying targeted mutations in genes affecting biological responses to DNA damage Version 7. DNA Repair (Amst). 2006, 5: 189-209. 10.1016\/j.dnarep.2005.09.009","journal-title":"DNA Repair (Amst)"},{"key":"125_CR16","doi-asserted-by":"publisher","first-page":"2391","DOI":"10.1128\/MCB.12.5.2391","volume":"12","author":"RM Mortensen","year":"1992","unstructured":"Mortensen RM, Conner DA, Chao S, Geisterfer-Lowrance AA, Seidman JG: Production of homozygous mutant ES cells with a single targeting construct. Mol Cell Biol. 1992, 12: 2391-2395.","journal-title":"Mol Cell Biol"},{"key":"125_CR17","doi-asserted-by":"publisher","first-page":"3586","DOI":"10.1073\/pnas.062527199","volume":"99","author":"RB Cervantes","year":"2002","unstructured":"Cervantes RB, Stringer JR, Shao C, Tischfield JA, Stambrook PJ: Embryonic stem cells and somatic cells differ in mutation frequency and type. Proc Natl Acad Sci U S A. 2002, 99: 3586-3590. 10.1073\/pnas.062527199","journal-title":"Proc Natl Acad Sci U S A"},{"key":"125_CR18","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1038\/73563","volume":"24","author":"RJ Munroe","year":"2000","unstructured":"Munroe RJ, Bergstrom RA, Zheng QY, Libby B, Smith R, John SW, Schimenti KJ, Browning VL, Schimenti JC: Mouse mutants from chemically mutagenized embryonic stem cells. Nat Genet. 2000, 24: 318-321. 10.1038\/73563","journal-title":"Nat Genet"},{"key":"125_CR19","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1634\/stemcells.19-2-118","volume":"19","author":"TS Hawley","year":"2001","unstructured":"Hawley TS, Telford WG, Hawley RG: \"Rainbow\" reporters for multispectral marking and lineage analysis of hematopoietic stem cells. Stem Cells. 2001, 19: 118-124. 10.1634\/stemcells.19-2-118","journal-title":"Stem Cells"},{"key":"125_CR20","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1126\/science.1070919","volume":"297","author":"MB Elowitz","year":"2002","unstructured":"Elowitz MB, Levine AJ, Siggia ED, Swain PS: Stochastic gene expression in a single cell. Science. 2002, 297: 1183-1186. 10.1126\/science.1070919","journal-title":"Science"},{"key":"125_CR21","doi-asserted-by":"publisher","first-page":"1811","DOI":"10.1126\/science.1098641","volume":"304","author":"JM Raser","year":"2004","unstructured":"Raser JM, O'Shea EK: Control of stochasticity in eukaryotic gene expression. Science. 2004, 304: 1811-1814. 10.1126\/science.1098641","journal-title":"Science"},{"key":"125_CR22","doi-asserted-by":"publisher","first-page":"712","DOI":"10.1006\/bbrc.1999.0954","volume":"260","author":"HS Liu","year":"1999","unstructured":"Liu HS, Jan MS, Chou CK, Chen PH, Ke NJ: Is green fluorescent protein toxic to the living cells?. Biochem Biophys Res Commun. 1999, 260: 712-717. 10.1006\/bbrc.1999.0954","journal-title":"Biochem Biophys Res Commun"},{"key":"125_CR23","first-page":"743","volume":"30","author":"WN Zhao","year":"2003","unstructured":"Zhao WN, Meng GL, Xue YF: Labeling of three different mouse ES cell lines with the green fluorescent protein. Yi Chuan Xue Bao. 2003, 30: 743-749.","journal-title":"Yi Chuan Xue Bao"},{"key":"125_CR24","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1002\/(SICI)1098-2795(199909)54:1<43::AID-MRD6>3.0.CO;2-N","volume":"54","author":"M Kato","year":"1999","unstructured":"Kato M, Yamanouchi K, Ikawa M, Okabe M, Naito K, Tojo H: Efficient selection of transgenic mouse embryos using EGFP as a marker gene. Mol Reprod Dev. 1999, 54: 43-48. 10.1002\/(SICI)1098-2795(199909)54:1<43::AID-MRD6>3.0.CO;2-N","journal-title":"Mol Reprod Dev"},{"key":"125_CR25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0070-2153(08)60465-2","volume":"44","author":"M Ikawa","year":"1999","unstructured":"Ikawa M, Yamada S, Nakanishi T, Okabe M: Green fluorescent protein (GFP) as a vital marker in mammals. Curr Top Dev Biol. 1999, 44: 1-20.","journal-title":"Curr Top Dev Biol"},{"key":"125_CR26","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/S0014-5793(98)00593-6","volume":"430","author":"M Ikawa","year":"1998","unstructured":"Ikawa M, Yamada S, Nakanishi T, Okabe M: 'Green mice' and their potential usage in biological research. FEBS Lett. 1998, 430: 83-87. 10.1016\/S0014-5793(98)00593-6","journal-title":"FEBS Lett"},{"key":"125_CR27","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1038\/nrg1126","volume":"4","author":"AK Hadjantonakis","year":"2003","unstructured":"Hadjantonakis AK, Dickinson ME, Fraser SE, Papaioannou VE: Technicolour transgenics: imaging tools for functional genomics in the mouse. Nat Rev Genet. 2003, 4: 613-625. 10.1038\/nrg1126","journal-title":"Nat Rev Genet"},{"key":"125_CR28","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1007\/s10528-005-5223-6","volume":"43","author":"J Klysik","year":"2005","unstructured":"Klysik J, Singer JD: Mice with the enhanced green fluorescent protein gene knocked in to chromosome 11 exhibit normal transmission ratios. Biochem Genet. 2005, 43: 321-333. 10.1007\/s10528-005-5223-6","journal-title":"Biochem Genet"},{"key":"125_CR29","doi-asserted-by":"publisher","first-page":"1030","DOI":"10.1016\/j.bbrc.2003.11.184","volume":"313","author":"V Devgan","year":"2004","unstructured":"Devgan V, Rao MR, Seshagiri PB: Impact of embryonic expression of enhanced green fluorescent protein on early mouse development. Biochem Biophys Res Commun. 2004, 313: 1030-1036. 10.1016\/j.bbrc.2003.11.184","journal-title":"Biochem Biophys Res Commun"},{"key":"125_CR30","first-page":"xvi, 925","volume-title":"The genetics of bacteria and their viruses; studies in basic genetics and molecular biology","author":"W Hayes","year":"1968","unstructured":"Hayes W: The genetics of bacteria and their viruses; studies in basic genetics and molecular biology. 1968, xvi, 925-New York, , Wiley, 2d","edition":"2d"},{"key":"125_CR31","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1126\/science.6116279","volume":"214","author":"BI Eisenstein","year":"1981","unstructured":"Eisenstein BI: Phase variation of type 1 fimbriae in Escherichia coli is under transcriptional control. Science. 1981, 214: 337-339. 10.1126\/science.6116279","journal-title":"Science"},{"key":"125_CR32","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1038\/nature02646","volume":"429","author":"K Yusa","year":"2004","unstructured":"Yusa K, Horie K, Kondoh G, Kouno M, Maeda Y, Kinoshita T, Takeda J: Genome-wide phenotype analysis in ES cells by regulated disruption of Bloom's syndrome gene. Nature. 2004, 429: 896-899. 10.1038\/nature02646","journal-title":"Nature"},{"key":"125_CR33","doi-asserted-by":"publisher","first-page":"7587","DOI":"10.1128\/MCB.21.22.7587-7600.2001","volume":"21","author":"MF Chan","year":"2001","unstructured":"Chan MF, van Amerongen R, Nijjar T, Cuppen E, Jones PA, Laird PW: Reduced rates of gene loss, gene silencing, and gene mutation in Dnmt1-deficient embryonic stem cells. Mol Cell Biol. 2001, 21: 7587-7600. 10.1128\/MCB.21.22.7587-7600.2001","journal-title":"Mol Cell Biol"},{"key":"125_CR34","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1038\/82548","volume":"26","author":"G Luo","year":"2000","unstructured":"Luo G, Santoro IM, McDaniel LD, Nishijima I, Mills M, Youssoufian H, Vogel H, Schultz RA, Bradley A: Cancer predisposition caused by elevated mitotic recombination in Bloom mice. Nat Genet. 2000, 26: 424-429. 10.1038\/82548","journal-title":"Nat Genet"},{"key":"125_CR35","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.mrfmmm.2005.01.021","volume":"574","author":"V Borgdorff","year":"2005","unstructured":"Borgdorff V, van Hees-Stuivenberg S, Meijers CM, de Wind N: Spontaneous and mutagen-induced loss of DNA mismatch repair in Msh2-heterozygous mammalian cells. Mutat Res. 2005, 574: 50-57.","journal-title":"Mutat Res"},{"key":"125_CR36","first-page":"31","volume":"56","author":"A Sugawara","year":"2006","unstructured":"Sugawara A, Goto K, Sotomaru Y, Sofuni T, Ito T: Current status of chromosomal abnormalities in mouse embryonic stem cell lines used in Japan. Comp Med. 2006, 56: 31-34.","journal-title":"Comp Med"},{"key":"125_CR37","doi-asserted-by":"publisher","first-page":"7405","DOI":"10.1073\/pnas.97.13.7405","volume":"97","author":"C Shao","year":"2000","unstructured":"Shao C, Deng L, Henegariu O, Liang L, Stambrook PJ, Tischfield JA: Chromosome instability contributes to loss of heterozygosity in mice lacking p53. Proc Natl Acad Sci U S A. 2000, 97: 7405-7410. 10.1073\/pnas.97.13.7405","journal-title":"Proc Natl Acad Sci U S A"},{"key":"125_CR38","doi-asserted-by":"publisher","first-page":"11642","DOI":"10.1073\/pnas.0510741103","volume":"103","author":"SL Donahue","year":"2006","unstructured":"Donahue SL, Lin Q, Cao S, Ruley HE: Carcinogens induce genome-wide loss of heterozygosity in normal stem cells without persistent chromosomal instability. Proc Natl Acad Sci U S A. 2006, 103: 11642-11646. 10.1073\/pnas.0510741103","journal-title":"Proc Natl Acad Sci U S A"},{"key":"125_CR39","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1007\/BF02631066","volume":"28A","author":"DG Brown","year":"1992","unstructured":"Brown DG, Willington MA, Findlay I, Muggleton-Harris AL: Criteria that optimize the potential of murine embryonic stem cells for in vitro and in vivo developmental studies. In Vitro Cell Dev Biol. 1992, 28A: 773-778.","journal-title":"In Vitro Cell Dev Biol"},{"key":"125_CR40","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1242\/jcs.112.16.2705","volume":"112 ( Pt 16)","author":"EM Burns","year":"1999","unstructured":"Burns EM, Christopoulou L, Corish P, Tyler-Smith C: Quantitative measurement of mammalian chromosome mitotic loss rates using the green fluorescent protein. J Cell Sci. 1999, 112 ( Pt 16): 2705-2714.","journal-title":"J Cell Sci"},{"key":"125_CR41","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1111\/j.1469-7580.2006.00608.x","volume":"209","author":"JB Gross","year":"2006","unstructured":"Gross JB, Hanken J, Oglesby E, Marsh-Armstrong N: Use of a ROSA26:GFP transgenic line for long-term Xenopus fate-mapping studies. J Anat. 2006, 209: 401-413. 10.1111\/j.1469-7580.2006.00608.x","journal-title":"J Anat"},{"key":"125_CR42","doi-asserted-by":"publisher","first-page":"1712","DOI":"10.1158\/0008-5472.CAN-05-3138","volume":"66","author":"TV Kalin","year":"2006","unstructured":"Kalin TV, Wang IC, Ackerson TJ, Major ML, Detrisac CJ, Kalinichenko VV, Lyubimov A, Costa RH: Increased levels of the FoxM1 transcription factor accelerate development and progression of prostate carcinomas in both TRAMP and LADY transgenic mice. Cancer Res. 2006, 66: 1712-1720. 10.1158\/0008-5472.CAN-05-3138","journal-title":"Cancer Res"},{"key":"125_CR43","doi-asserted-by":"publisher","first-page":"1875","DOI":"10.1016\/S0002-9440(10)63240-3","volume":"165","author":"JE Kim","year":"2004","unstructured":"Kim JE, Nakashima K, de Crombrugghe B: Transgenic mice expressing a ligand-inducible cre recombinase in osteoblasts and odontoblasts: a new tool to examine physiology and disease of postnatal bone and tooth. Am J Pathol. 2004, 165: 1875-1882.","journal-title":"Am J Pathol"},{"key":"125_CR44","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1002\/gene.20043","volume":"39","author":"T Kusakabe","year":"2004","unstructured":"Kusakabe T, Kawaguchi A, Kawaguchi R, Feigenbaum L, Kimura S: Thyrocyte-specific expression of Cre recombinase in transgenic mice. Genesis. 2004, 39: 212-216. 10.1002\/gene.20043","journal-title":"Genesis"},{"key":"125_CR45","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1023\/B:TRAG.0000034716.73957.f7","volume":"13","author":"SS Chung","year":"2004","unstructured":"Chung SS, Cuzin F, Rassoulzadegan M, Wolgemuth DJ: Primary spermatocyte-specific Cre recombinase activity in transgenic mice. Transgenic Res. 2004, 13: 289-294. 10.1023\/B:TRAG.0000034716.73957.f7","journal-title":"Transgenic Res"},{"key":"125_CR46","doi-asserted-by":"publisher","first-page":"1180","DOI":"10.1161\/01.ATV.0000129330.19057.9f","volume":"24","author":"BC Cooley","year":"2004","unstructured":"Cooley BC: Murine model of neointimal formation and stenosis in vein grafts. Arterioscler Thromb Vasc Biol. 2004, 24: 1180-1185. 10.1161\/01.ATV.0000129330.19057.9f","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"125_CR47","doi-asserted-by":"publisher","first-page":"37888","DOI":"10.1074\/jbc.M305555200","volume":"278","author":"VV Kalinichenko","year":"2003","unstructured":"Kalinichenko VV, Gusarova GA, Tan Y, Wang IC, Major ML, Wang X, Yoder HM, Costa RH: Ubiquitous expression of the forkhead box M1B transgene accelerates proliferation of distinct pulmonary cell types following lung injury. J Biol Chem. 2003, 278: 37888-37894. 10.1074\/jbc.M305555200","journal-title":"J Biol Chem"},{"key":"125_CR48","doi-asserted-by":"publisher","first-page":"1058","DOI":"10.1007\/s003350010208","volume":"11","author":"RL Kohlhepp","year":"2000","unstructured":"Kohlhepp RL, Hegge LF, Nett JE, Moser AR: ROSA26 mice carry a modifier of Min-induced mammary and intestinal tumor development. Mamm Genome. 2000, 11: 1058-1062. 10.1007\/s003350010208","journal-title":"Mamm Genome"},{"key":"125_CR49","doi-asserted-by":"crossref","first-page":"3755","DOI":"10.1242\/dev.124.19.3755","volume":"124","author":"N Narita","year":"1997","unstructured":"Narita N, Bielinska M, Wilson DB: Cardiomyocyte differentiation by GATA-4-deficient embryonic stem cells. Development. 1997, 124: 3755-3764.","journal-title":"Development"},{"key":"125_CR50","doi-asserted-by":"publisher","first-page":"2641","DOI":"10.1016\/S0022-5347(05)64235-8","volume":"168","author":"A Bex","year":"2002","unstructured":"Bex A, Vooijs M, Horenblas S, Berns A: Controlling gene expression in the urothelium using transgenic mice with inducible bladder specific Cre-lox recombination. J Urol. 2002, 168: 2641-2644. 10.1016\/S0022-5347(05)64235-8","journal-title":"J Urol"},{"key":"125_CR51","first-page":"x, 764","volume-title":"Manipulating the mouse embryo : a laboratory manual","author":"A Nagy","year":"2003","unstructured":"Nagy A: Manipulating the mouse embryo : a laboratory manual. 2003, x, 764-Cold Spring Harbor, N.Y., Cold Spring Harbor Laboratory Press, 3rd","edition":"3rd"},{"key":"125_CR52","unstructured":"[www.informatics.jax.org] MGI: ."},{"key":"125_CR53","unstructured":"[www.polymorphicdna.com] PDNAT: ."},{"key":"125_CR54","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1002\/1097-0320(20010201)43:2<101::AID-CYTO1024>3.0.CO;2-8","volume":"43","author":"O Henegariu","year":"2001","unstructured":"Henegariu O, Heerema NA, Lowe Wright L, Bray-Ward P, Ward DC, Vance GH: Improvements in cytogenetic slide preparation: controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry. 2001, 43: 101-109. 10.1002\/1097-0320(20010201)43:2<101::AID-CYTO1024>3.0.CO;2-8","journal-title":"Cytometry"}],"container-title":["BMC Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2199-7-36.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T10:55:13Z","timestamp":1630493713000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmolbiol.biomedcentral.com\/articles\/10.1186\/1471-2199-7-36"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,16]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["125"],"URL":"https:\/\/doi.org\/10.1186\/1471-2199-7-36","relation":{},"ISSN":["1471-2199"],"issn-type":[{"value":"1471-2199","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10,16]]},"assertion":[{"value":"15 June 2006","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 October 2006","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 October 2006","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"36"}}