{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:45:20Z","timestamp":1792651520300,"version":"4.5.0"},"reference-count":67,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Neurosci"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Nicotinic acetylcholine receptors (nAChRs) play an important role as excitatory neurotransmitters in vertebrate and invertebrate species. In insects, nAChRs are the site of action of commercially important insecticides and, as a consequence, there is considerable interest in examining their functional properties. However, problems have been encountered in the successful functional expression of insect nAChRs, although a number of strategies have been developed in an attempt to overcome such difficulties. Ten nAChR subunits have been identified in the model insect <jats:italic>Drosophila melanogaster<\/jats:italic> (D\u03b11-D\u03b17 and D\u03b21-D\u03b23) and a similar number have been identified in other insect species. The focus of the present study is the D\u03b15, D\u03b16 and D\u03b17 subunits, which are distinguished by their sequence similarity to one another and also by their close similarity to the vertebrate \u03b17 nAChR subunit.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>A full-length cDNA clone encoding the <jats:italic>Drosophila<\/jats:italic> nAChR D\u03b15 subunit has been isolated and the properties of D\u03b15-, D\u03b16- and D\u03b17-containing nAChRs examined in a variety of cell expression systems. We have demonstrated the functional expression, as homomeric nAChRs, of the D\u03b15 and D\u03b17 subunits in <jats:italic>Xenopus<\/jats:italic> oocytes by their co-expression with the molecular chaperone RIC-3. Also, using a similar approach, we have demonstrated the functional expression of a heteromeric \u2018triplet\u2019 nAChR (D\u03b15\u2009+\u2009D\u03b16\u2009+\u2009D\u03b17) with substantially higher apparent affinity for acetylcholine than is seen with other subunit combinations. In addition, specific cell-surface binding of [<jats:sup>125<\/jats:sup>I]-\u03b1-bungarotoxin was detected in both <jats:italic>Drosophila<\/jats:italic> and mammalian cell lines when D\u03b15 was co-expressed with D\u03b16 and RIC-3. In contrast, co-expression of additional subunits (including D\u03b17) with D\u03b15 and D\u03b16 prevented specific binding of [<jats:sup>125<\/jats:sup>I]-\u03b1-bungarotoxin in cell lines, suggesting that co-assembly with other nAChR subunits can block maturation of correctly folded nAChRs in some cellular environments.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Data are presented demonstrating the ability of the <jats:italic>Drosophila<\/jats:italic> D\u03b15 and D\u03b17 subunits to generate functional homomeric and also heteromeric nAChRs.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2202-13-73","type":"journal-article","created":{"date-parts":[[2012,9,17]],"date-time":"2012-09-17T16:37:40Z","timestamp":1347899860000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["The Drosophila nicotinic acetylcholine receptor subunits D\u03b15 and D\u03b17 form functional homomeric and heteromeric ion channels"],"prefix":"10.1186","volume":"13","author":[{"given":"Stuart J","family":"Lansdell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toby","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Goodchild","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil S","family":"Millar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,6,22]]},"reference":[{"key":"2760_CR1","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/s10158-006-0040-0","volume":"7","author":"NS Millar","year":"2007","unstructured":"Millar NS, Denholm I: Nicotinic acetylcholine receptors: targets for commercially important insecticides. Invert Neurosci. 2007, 7: 53-66.","journal-title":"Invert Neurosci"},{"key":"2760_CR2","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1007\/s10158-006-0039-6","volume":"7","author":"AK Jones","year":"2007","unstructured":"Jones AK, Brown AM, Sattelle DB: Insect nicotinic acetylcholine receptor gene families: from genetic model organisms to vector, pest and beneficial species. Invert Neurosci. 2007, 7: 67-73.","journal-title":"Invert Neurosci"},{"key":"2760_CR3","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1016\/j.jmb.2004.12.031","volume":"346","author":"N Unwin","year":"2005","unstructured":"Unwin N: Refined structure of the nicotinic acetylcholine receptor at 4\u2009\u00c5 resolution. J Mol Biol. 2005, 346: 967-989.","journal-title":"J Mol Biol"},{"key":"2760_CR4","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1038\/nn1942","volume":"10","author":"CD Dellisanti","year":"2007","unstructured":"Dellisanti CD, Yao Y, Stroud JC, Wang Z-Z, Chen L: Crystal structure of the extracellular domain of nAChR \u03b11 bound to \u03b1-bungarotoxin at 1.94\u00c5 resolution. Nature Neurosci. 2007, 10: 953-962.","journal-title":"Nature Neurosci"},{"key":"2760_CR5","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1038\/35077011","volume":"411","author":"K Brejc","year":"2001","unstructured":"Brejc K, van Dijk WJ, Klaassen RV, Schuurmans M, van der Oost J, Smit AB, Sixma TK: Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors. Nature. 2001, 411: 269-276.","journal-title":"Nature"},{"key":"2760_CR6","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1038\/nrd2927","volume":"8","author":"A Taly","year":"2009","unstructured":"Taly A, Corringer P-J, Guedin D, Lestage P, Changeux J-P: Nicotinic receptors: allosteric transitions and therapeutic targets in the nervous system. Nature Rev Drug Discovery. 2009, 8: 733-750.","journal-title":"Nature Rev Drug Discovery"},{"key":"2760_CR7","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1152\/physrev.00015.2008","volume":"89","author":"EX Albuquerque","year":"2009","unstructured":"Albuquerque EX, Pereira FR, Alkondon M, Rogers SW: Mammalian nicotinic acetylcholine receptors: from structure to function. Physiol Rev. 2009, 89: 73-120.","journal-title":"Physiol Rev"},{"key":"2760_CR8","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/S0197-0186(99)00154-0","volume":"36","author":"HR Arias","year":"2000","unstructured":"Arias HR: Localization of agonist and competitive antagonist binding sites on nicotinic acetylcholine receptors. Neurochem Int. 2000, 36: 595-645.","journal-title":"Neurochem Int"},{"key":"2760_CR9","doi-asserted-by":"publisher","first-page":"5867","DOI":"10.1073\/pnas.1017975108","volume":"108","author":"JK Gill","year":"2011","unstructured":"Gill JK, Savolainen M, Young GT, Zwart R, Sher E, Millar NS: Agonist activation of \u03b17 nicotinic acetylcholine receptors via an allosteric transmembrane site. Proc Natl Acad Sci USA. 2011, 108: 5867-5872.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2760_CR10","doi-asserted-by":"publisher","first-page":"944","DOI":"10.1042\/bst0270944","volume":"27","author":"NS Millar","year":"1999","unstructured":"Millar NS: Heterologous expression of mammalian and insect neuronal nicotinic acetylcholine receptors in cultured cell lines. Biochem Soc Trans. 1999, 27: 944-950.","journal-title":"Biochem Soc Trans"},{"key":"2760_CR11","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1016\/j.bcp.2009.06.015","volume":"78","author":"NS Millar","year":"2009","unstructured":"Millar NS: A review of experimental techniques used for the heterologous expression of nicotinic acetylcholine receptors. Biochem Pharmacol. 2009, 78: 766-776.","journal-title":"Biochem Pharmacol"},{"key":"2760_CR12","doi-asserted-by":"crossref","first-page":"4391","DOI":"10.1002\/j.1460-2075.1990.tb07889.x","volume":"9","author":"J Marshall","year":"1990","unstructured":"Marshall J, Buckingham SD, Shingai R, Lunt GG, Goosey MW, Darlison MG, Sattelle DB, Barnard EA: Sequence and functional expression of a single \u03b1 subunit of an insect nicotinic acetylcholine receptor. EMBO J. 1990, 9: 4391-4398.","journal-title":"EMBO J"},{"key":"2760_CR13","doi-asserted-by":"crossref","first-page":"2671","DOI":"10.1002\/j.1460-2075.1990.tb07452.x","volume":"9","author":"E Sawruk","year":"1990","unstructured":"Sawruk E, Schloss P, Betz H, Schmitt B: Heterogeneity of Drosophila nicotinic acetylcholine receptors: SAD, a novel developmentally regulated \u03b1-subunit. EMBO J. 1990, 9: 2671-2677.","journal-title":"EMBO J"},{"key":"2760_CR14","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1046\/j.1471-4159.1998.71030903.x","volume":"71","author":"F Sgard","year":"1998","unstructured":"Sgard F, Fraser SP, Katkowska MJ, Djamgoz MBA, Dunbar SJ, Windass JD: Cloning and functional characterisation of two novel nicotinic acetylcholine receptor \u03b1 subunits from the insect pest Myzus persicae. J Neurochem. 1998, 71: 903-912.","journal-title":"J Neurochem"},{"key":"2760_CR15","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1111\/j.1471-4159.2004.02499.x","volume":"90","author":"SJ Lansdell","year":"2004","unstructured":"Lansdell SJ, Millar NS: Molecular characterisation of D\u03b16 and D\u03b17 nicotinic acetylcholine receptor subunits from Drosophila: formation of a high-affinity \u03b1-bungarotoxin binding site revealed by expression of subunit chimeras. J Neurochem. 2004, 90: 479-489.","journal-title":"J Neurochem"},{"key":"2760_CR16","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1111\/j.1460-9568.1994.tb00997.x","volume":"6","author":"D Bertrand","year":"1994","unstructured":"Bertrand D, Ballivet M, Gomez M, Bertrand S, Phannavong B, Gundelfinger ED: Physiological properties of neuronal nicotinic receptors reconstituted from the vertebrate \u03b22 subunit and Drosophila \u03b1 subunits. Eur J Neurosci. 1994, 6: 869-875.","journal-title":"Eur J Neurosci"},{"key":"2760_CR17","doi-asserted-by":"publisher","first-page":"1812","DOI":"10.1046\/j.1471-4159.1997.68051812.x","volume":"68","author":"SJ Lansdell","year":"1997","unstructured":"Lansdell SJ, Schmitt B, Betz H, Sattelle DB, Millar NS: Temperature-sensitive expression of Drosophila neuronal nicotinic acetylcholine receptors. J Neurochem. 1997, 68: 1812-1819.","journal-title":"J Neurochem"},{"key":"2760_CR18","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.ibmb.2005.11.003","volume":"36","author":"C Bass","year":"2006","unstructured":"Bass C, Lansdell SJ, Millar NS, Schroeder I, Turberg A, Field LM, Williamson MS: Molecular characterisation of nicotinic acetylcholine receptor subunits from the cat flea, Ctenocephalides felis (Siphonaptera: Pulicidae). Insect Biochem Mol Biol. 2006, 36: 86-96.","journal-title":"Insect Biochem Mol Biol"},{"key":"2760_CR19","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1046\/j.1471-4159.1999.0730380.x","volume":"73","author":"Y Huang","year":"1999","unstructured":"Huang Y, Williamson MS, Devonshire AL, Windass JD, Lansdell SJ, Millar NS: Molecular characterization and imidacloprid selectivity of nicotinic acetylcholine receptor subunits from the peach-potato aphid Myzus persicae. J Neurochem. 1999, 73: 380-389.","journal-title":"J Neurochem"},{"key":"2760_CR20","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/S0304-3940(00)00969-1","volume":"284","author":"Y Huang","year":"2000","unstructured":"Huang Y, Williamson MS, Devonshire AL, Windass JD, Lansdell SJ, Millar NS: Cloning, heterologous expression and co-assembly of Mp\u03b21, a nicotinic acetylcholine receptor subunit from the aphid Myzus persicae. Neurosci Lett. 2000, 284: 116-120.","journal-title":"Neurosci Lett"},{"key":"2760_CR21","doi-asserted-by":"publisher","first-page":"8420","DOI":"10.1073\/pnas.0502901102","volume":"102","author":"Z Liu","year":"2005","unstructured":"Liu Z, Williamson MS, Lansdell SJ, Denholm I, Han Z, Millar NS: A nicotinic acetylcholine receptor mutation conferring target-site resistance to imidacloprid in Nilaparvata lugens (brown planthopper). Proc Natl Acad Sci USA. 2005, 102: 8420-8425.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2760_CR22","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.1111\/j.1471-4159.2006.04167.x","volume":"99","author":"Z Liu","year":"2006","unstructured":"Liu Z, Williamson MS, Lansdell SJ, Han Z, Denholm I, Millar NS: A nicotinic acetylcholine receptor mutation (Y151S) causes reduced agonist potency to a range of neonicotinoid insecticides. J Neurochem. 2006, 99: 1273-1281.","journal-title":"J Neurochem"},{"key":"2760_CR23","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1046\/j.1365-2583.2003.00409.x","volume":"12","author":"SH Thany","year":"2003","unstructured":"Thany SH, Lenaers G, Crozatier M, Armengaud C, Gauthier M: Identification and localization of the nicotinic acetylcholine receptor alpha3 mRNA in the brain of the honeybee, Apis mellifera. Insect Mol Biol. 2003, 12: 255-262.","journal-title":"Insect Mol Biol"},{"key":"2760_CR24","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.gene.2004.09.010","volume":"344","author":"SH Thany","year":"2005","unstructured":"Thany SH, Crozatier M, Raymond-Delpech V, Gauthier M, Lenaers G: Apis\u03b12, Apis\u03b17-1 and Apis\u03b17-2: three new neuronal nicotinic acetylcholine receptor \u03b1-subunits in the honeybee brain. Gene. 2005, 344: 125-132.","journal-title":"Gene"},{"key":"2760_CR25","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1186\/1471-2148-7-98","volume":"7","author":"Y Jin","year":"2007","unstructured":"Jin Y, Tian N, Cao J, Liang J, Yang Z, Lv J: RNA editing and alternative splicing of the insect nAChR subunit alpha6 transcript: evolutionary conservation, divergence and regulation. BMC Evolution Biol. 2007, 7: 98-10.1186\/1471-2148-1187-1198.","journal-title":"BMC Evolution Biol"},{"key":"2760_CR26","doi-asserted-by":"publisher","first-page":"e1000802","DOI":"10.1371\/journal.pgen.1000802","volume":"6","author":"SW Baxter","year":"2010","unstructured":"Baxter SW, Chen M, Dawson A, Zhao J-Z, Vogel H, Shelton AM, Heckel DG, Jiggins CD: Mis-spliced transcripts of nicotinic acetylcholine receptor \u03b16 are associated with field evolved spinosad resistance in Plutella xylostells (L.). PLoS Genetics. 2010, 6: e1000802-1000810.1000137\/journal.pgen.1000802.","journal-title":"PLoS Genetics"},{"key":"2760_CR27","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/s10158-010-0102-1","volume":"10","author":"FD Rinkevich","year":"2010","unstructured":"Rinkevich FD, Chen M, Shelton AM, Scott JG: Transcripts of the nicotinic acetylcholine receptor subunit gene Pxyl\u03b16 with premature stop codons are associated with spinosad resistance in diamondback moth, Plutella xylostella. Invert Neurosci. 2010, 10: 25-33.","journal-title":"Invert Neurosci"},{"key":"2760_CR28","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1111\/j.1365-2583.2007.00770.x","volume":"16","author":"J-R Gao","year":"2007","unstructured":"Gao J-R, Deacutis JM, Scott JG: The nicotinic acetylcholine receptor subunit Md\u03b15 and Md\u03b23 on autosome 1 of Musca domestica are not involved in spinosad resistance. Insect Mol Biol. 2007, 16: 691-701.","journal-title":"Insect Mol Biol"},{"key":"2760_CR29","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1002\/arch.20158","volume":"64","author":"J-R Gao","year":"2007","unstructured":"Gao J-R, Deacutis JM, Scott JG: Characterization of the nicotinic acetylcholine receptor gene Md\u03b12 from the house fly Musca domestica. Arch Insect Biochem Physiol. 2007, 64: 30-42.","journal-title":"Arch Insect Biochem Physiol"},{"key":"2760_CR30","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1111\/j.1365-2583.2007.00730.x","volume":"16","author":"J-R Gao","year":"2007","unstructured":"Gao J-R, Deacutis JM, Scott JG: The nicotinic acetylcholine receptor subunit Md\u03b16 from Musca domestica is diversified via post-transcriptional modification. Insect Mol Biol. 2007, 16: 325-334.","journal-title":"Insect Mol Biol"},{"key":"2760_CR31","doi-asserted-by":"publisher","first-page":"18394","DOI":"10.1074\/jbc.273.29.18394","volume":"273","author":"B Hermsen","year":"1998","unstructured":"Hermsen B, Stetzer E, Thees R, Heiermann R, Schrattenholz A, Ebbinghaus U, Kretschmer A, Methfessel C, Reinhardt S, Maelicke A: Neuronal nicotinic receptors in the Locust Locusta migratoria: cloning and expression. J Biol Chem. 1998, 273: 18394-18404.","journal-title":"J Biol Chem"},{"key":"2760_CR32","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1111\/j.1365-2583.2009.00873.x","volume":"18","author":"FD Rinkevich","year":"2009","unstructured":"Rinkevich FD, Scott JG: Transcriptional diversity and allelic variation in nicotinic acetylcholine receptor subunits of the red flour beetle, Tribolium castaneum. Insect Mol Biol. 2009, 18: 233-242.","journal-title":"Insect Mol Biol"},{"key":"2760_CR33","first-page":"324","volume":"8","author":"Y-M Shao","year":"2007","unstructured":"Shao Y-M, Dong K, Zhang C-X: The nicotinic acetylcholine receptor gene family of the silkworm. Bombyx mori. BMC Genomics. 2007, 8: 324-310.1186\/1471-2164-1188-1324.","journal-title":"Bombyx mori. BMC Genomics"},{"key":"2760_CR34","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1046\/j.1460-9568.1998.00095.x","volume":"10","author":"HM Eastham","year":"1998","unstructured":"Eastham HM, Lind RJ, Eastlake JL, Clarke BS, Towner P, Reynolds SE, Wolstenholme AJ, Wonnacott S: Characterization of a nicotinic acetylcholine receptor from the insect Manduca sexta. Eur J Neurosci. 1998, 10: 879-889.","journal-title":"Eur J Neurosci"},{"key":"2760_CR35","doi-asserted-by":"publisher","first-page":"1012","DOI":"10.1093\/emboj\/21.5.1012","volume":"21","author":"S Halevi","year":"2002","unstructured":"Halevi S, McKay J, Palfreyman M, Yassin L, Eshel M, Jorgensen E, Treinin M: The C. elegans ric-3 gene is required for maturation of nicotinic acetylcholine receptors. EMBO J. 2002, 21: 1012-1020.","journal-title":"EMBO J"},{"key":"2760_CR36","doi-asserted-by":"publisher","first-page":"S177","DOI":"10.1038\/sj.bjp.0707661","volume":"153","author":"NS Millar","year":"2008","unstructured":"Millar NS: RIC-3: a nicotinic acetylcholine receptor chaperone. Br J Pharmacol. 2008, 153: S177-S183.","journal-title":"Br J Pharmacol"},{"key":"2760_CR37","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.1074\/jbc.M410039200","volume":"280","author":"ME Williams","year":"2005","unstructured":"Williams ME, Burton B, Urrutia A, Shcherbatko A, Chavez-Noriega LE, Cohen CJ, Aiyar J: Ric-3 promotes functional expression of the nicotinic acetylcholine receptor \u03b17 subunit in mammalian cells. J Biol Chem. 2005, 280: 1257-1263.","journal-title":"J Biol Chem"},{"key":"2760_CR38","doi-asserted-by":"publisher","first-page":"1431","DOI":"10.1124\/mol.105.017459","volume":"68","author":"SJ Lansdell","year":"2005","unstructured":"Lansdell SJ, Gee VJ, Harkness PC, Doward AI, Baker ER, Gibb AJ, Millar NS: RIC-3 enhances functional expression of multiple nicotinic acetylcholine receptor subtypes in mammalian cells. Mol Pharmacol. 2005, 68: 1431-1438.","journal-title":"Mol Pharmacol"},{"key":"2760_CR39","doi-asserted-by":"publisher","first-page":"1573","DOI":"10.1111\/j.1471-4159.2008.05235.x","volume":"105","author":"SJ Lansdell","year":"2008","unstructured":"Lansdell SJ, Collins T, Yabe A, Gee VJ, Gibb AJ, Millar NS: Host-cell specific effects of the nicotinic acetylcholine receptor chaperone RIC-3 revealed by a comparison of human and Drosophila RIC-3 homologues. J Neurochem. 2008, 105: 1573-1581.","journal-title":"J Neurochem"},{"key":"2760_CR40","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1016\/j.ibmb.2009.11.004","volume":"40","author":"GB Watson","year":"2010","unstructured":"Watson GB, Chouinard SW, Cook KR, Geng C, Gifford JM, Gustafson GD, Hasler JM, Larrinua IM, Letherer TJ, Mitchell JC, et al: A spinosyn-sensitive Drosophila melanogaster nicotinic acetylcholine receptor identified through chemically induced target site resistance, resistance gene identification, and heterologous expression. Insect Biochem Mol Biol. 2010, 40: 376-384.","journal-title":"Insect Biochem Mol Biol"},{"key":"2760_CR41","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1093\/genetics\/160.4.1519","volume":"160","author":"M Grauso","year":"2002","unstructured":"Grauso M, Reenan RA, Culetto E, Sattelle DB: Novel putative nicotinic acetylcholine receptor subunit genes, D\u03b15, D\u03b16 and D\u03b17, in Drosophila melanogaster identify a new and highly conserved target of adenosine deaminase acting on RNA-mediated A-to-I pre-mRNA editing. Genetics. 2002, 160: 1519-1533.","journal-title":"Genetics"},{"key":"2760_CR42","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/978-1-4419-6445-8_3","volume":"683","author":"AK Jones","year":"2009","unstructured":"Jones AK, Sattelle DB: Diversity of insect nicotinic acetylcholine receptor subunits. Adv Exp Med Biol. 2009, 683: 25-43.","journal-title":"Adv Exp Med Biol"},{"key":"2760_CR43","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1002\/bies.20207","volume":"27","author":"DB Sattelle","year":"2005","unstructured":"Sattelle DB, Jones AK, Sattelle BM, Matsuda K, Reenan R, Biggin PC: Edit, cut and paste in the nicotinic acetylcholine receptor gene family of Drosophila melanogaster. Bioessays. 2005, 27: 366-376.","journal-title":"Bioessays"},{"key":"2760_CR44","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1046\/j.1471-4159.1998.71020853.x","volume":"71","author":"R Schulz","year":"1998","unstructured":"Schulz R, Sawruk E, M\u00fclhardt C, Bertrand S, Baumann A, Phannavong B, Betz H, Bertrand D, Gundelfinger ED, Schmitt B: D\u03b13, a new functional a subunit of nicotinic acetylcholine receptors from Drosophila. J Neurochem. 1998, 71: 853-862.","journal-title":"J Neurochem"},{"key":"2760_CR45","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1016\/S0028-3908(99)00170-7","volume":"39","author":"SJ Lansdell","year":"2000","unstructured":"Lansdell SJ, Millar NS: The influence of nicotinic receptor subunit composition upon agonist, \u03b1-bungarotoxin and insecticide (imidacloprid) binding affinity. Neuropharmacol. 2000, 39: 671-679.","journal-title":"Neuropharmacol"},{"key":"2760_CR46","doi-asserted-by":"publisher","first-page":"20987","DOI":"10.1074\/jbc.M409639200","volume":"280","author":"P Wu","year":"2005","unstructured":"Wu P, Ma D, Pierzchala M, Wu J, Yang L-C, Mai X, Chang X, Schmidt-Glenewinkel T: The Drosophila acetylcholine receptor subunit D\u03b15 is part of an \u03b1-bungarotoxin binding acetylcholine receptor. J Biol Chem. 2005, 280: 20987-20994.","journal-title":"J Biol Chem"},{"key":"2760_CR47","doi-asserted-by":"publisher","first-page":"2585","DOI":"10.1046\/j.1471-4159.1998.70062585.x","volume":"70","author":"ST Cooper","year":"1998","unstructured":"Cooper ST, Millar NS: Host cell-specific folding of the neuronal nicotinic receptor \u03b18 subunit. J Neurochem. 1998, 70: 2585-2593.","journal-title":"J Neurochem"},{"key":"2760_CR48","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/S0896-6273(00)81135-6","volume":"26","author":"JT Littleton","year":"2000","unstructured":"Littleton JT, Ganetzky B: Ion channels and synaptic organization: analysis of the Drosophila genome. Neuron. 2000, 26: 35-43.","journal-title":"Neuron"},{"key":"2760_CR49","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1016\/0896-6273(90)90344-F","volume":"5","author":"S Couturier","year":"1990","unstructured":"Couturier S, Bertrand D, Matter JM, Hernandez MC, Bertrand S, Millar N, Valera S, Barkas T, Ballivet M: A neuronal nicotinic acetylcholine receptor subunit (\u03b17) is developmentally regulated and forms a homo-oligomeric channel blocked by \u03b1-BTX. Neuron. 1990, 5: 847-856.","journal-title":"Neuron"},{"key":"2760_CR50","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1111\/j.1460-9568.1997.tb01475.x","volume":"9","author":"C Gotti","year":"1997","unstructured":"Gotti C, Moretti M, Maggi R, Longhi R, Hanke W, Klinke N, Clementi F: \u03b17 and \u03b18 nicotinic receptor subtypes immunoprecipitated from chick retina have different immunological, pharmacological and functional properties. Eur J Neurosci. 1997, 9: 1201-1211.","journal-title":"Eur J Neurosci"},{"key":"2760_CR51","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1113\/jphysiol.2001.013847","volume":"540","author":"SS Khiroug","year":"2002","unstructured":"Khiroug SS, Harkness PC, Lamb PW, Sudweeks SN, Khiroug L, Millar NS, Yakel JL: Rat nicotinic receptor \u03b17 and \u03b22 subunits co-assemble to form functional heteromeric nicotinic receptor channels. J Physiol. 2002, 540: 425-434.","journal-title":"J Physiol"},{"key":"2760_CR52","doi-asserted-by":"publisher","first-page":"918","DOI":"10.1523\/JNEUROSCI.3952-08.2009","volume":"29","author":"Q Liu","year":"2009","unstructured":"Liu Q, Huang Y, Xue F, Simard A, DeChon J, Li G, Zhang J, Lucero L, Wang M, Sierks M, et al: A novel nicotinic acetylcholine receptor subtype in basal forebrain cholinergic neurons with high sensitivity to amyloid peptides. J Neurosci. 2009, 29: 918-929.","journal-title":"J Neurosci"},{"key":"2760_CR53","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1016\/j.ibmb.2006.11.009","volume":"37","author":"T Perry","year":"2007","unstructured":"Perry T, McKenzie JA, Batterham P: A D\u03b16 knockout strain of Drosophila melanogaster confers a high level of resistance to spinosad. Insect Biochem Mol Biol. 2007, 37: 184-188.","journal-title":"Insect Biochem Mol Biol"},{"issue":"3","key":"2760_CR54","doi-asserted-by":"publisher","first-page":"e63","DOI":"10.1371\/journal.pbio.0040063","volume":"4","author":"A Fayyazuddin","year":"2006","unstructured":"Fayyazuddin A, Zaheer MA, Hiesinger PR, Bellen HJ: The nicotinic acetylcholine receptor D\u03b17 is required for an escape behavior in Drosophila. PLoS Biol. 2006, 4 (3): e63.","journal-title":"PLoS Biol"},{"key":"2760_CR55","doi-asserted-by":"publisher","first-page":"2140","DOI":"10.1046\/j.1471-4159.1997.68052140.x","volume":"68","author":"ST Cooper","year":"1997","unstructured":"Cooper ST, Millar NS: Host cell-specific folding and assembly of the neuronal nicotinic acetylcholine receptor \u03b17 subunit. J Neurochem. 1997, 68: 2140-2151.","journal-title":"J Neurochem"},{"key":"2760_CR56","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1111\/j.1469-7793.1997.299bb.x","volume":"505","author":"TM Lewis","year":"1997","unstructured":"Lewis TM, Harkness PC, Sivilotti LG, Colquhoun D, Millar NS: The ion channel properties of a rat recombinant neuronal nicotinic receptor are dependent on the host cell type. J Physiol. 1997, 505: 299-306.","journal-title":"J Physiol"},{"issue":"5","key":"2760_CR57","doi-asserted-by":"publisher","first-page":"1705","DOI":"10.1083\/jcb.110.5.1705","volume":"110","author":"HL Paulson","year":"1990","unstructured":"Paulson HL, Claudio T: Temperature-sensitive expression of all-Torpedo and Torpedo-rat hybrid AChR in mammalian muscle cells. J Cell Biol. 1990, 110 (5): 1705-1717.","journal-title":"J Cell Biol"},{"key":"2760_CR58","doi-asserted-by":"publisher","first-page":"18590","DOI":"10.1073\/pnas.0806933105","volume":"105","author":"T Boulin","year":"2008","unstructured":"Boulin T, Gielen M, Richmond JE, Williams DC, Paoletti P, Bessereau J-L: Eight genes are required for functional reconstitution of the Caenorhabditis elegans levamisole-sensitive acetylcholine receptor. Proc Natl Acad Sci USA. 2008, 105: 18590-18595.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2760_CR59","doi-asserted-by":"publisher","first-page":"2604","DOI":"10.1016\/S0028-3908(00)00111-8","volume":"39","author":"SJ Lansdell","year":"2000","unstructured":"Lansdell SJ, Millar NS: Cloning and heterologous expression of D\u03b14, a Drosophila neuronal nicotinic acetylcholine receptor subunit: identification of an alternative exon influencing the efficiency of subunit assembly. Neuropharmacol. 2000, 39: 2604-2614.","journal-title":"Neuropharmacol"},{"key":"2760_CR60","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1046\/j.0022-3042.2002.00789.x","volume":"80","author":"SJ Lansdell","year":"2002","unstructured":"Lansdell SJ, Millar NS: D\u03b23, an atypical nicotinic acetylcholine receptor subunit from Drosophila: molecular cloning, heterologous expression and coassembly. J Neurochem. 2002, 80: 1009-1018.","journal-title":"J Neurochem"},{"key":"2760_CR61","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1016\/0896-6273(92)90239-A","volume":"9","author":"ER Liman","year":"1992","unstructured":"Liman ER, Tytgat J, Hess P: Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs. Neuron. 1992, 9: 861-871.","journal-title":"Neuron"},{"key":"2760_CR62","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1038\/366479a0","volume":"366","author":"J-L Eisel\u00e9","year":"1993","unstructured":"Eisel\u00e9 J-L, Bertrand S, Galzi J-L, Devillers-Thi\u00e9ry A, Changeux J-P, Bertrand D: Chimaeric nicotinic-serotonergic receptor combines distinct ligand binding and channel specificities. Nature. 1993, 366: 479-483.","journal-title":"Nature"},{"key":"2760_CR63","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1126\/science.1718042","volume":"254","author":"AV Maricq","year":"1991","unstructured":"Maricq AV, Peterson AS, Brake AJ, Myers RM, Julius D: Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel. Science. 1991, 254: 432-437.","journal-title":"Science"},{"key":"2760_CR64","first-page":"353","volume":"27","author":"I Schneider","year":"1972","unstructured":"Schneider I: Cell lines derived from late embryonic stages of Drosophila melanogaster. J Embryol Exp Morph. 1972, 27: 353-365.","journal-title":"J Embryol Exp Morph"},{"key":"2760_CR65","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1098\/rspb.1994.0178","volume":"258","author":"NS Millar","year":"1994","unstructured":"Millar NS, Buckingham SD, Sattelle DB: Stable expression of a functional homo-oligomeric Drosophila GABA receptor in a Drosophila cell line. Proc R Soc Lond B. 1994, 258: 307-314.","journal-title":"Proc R Soc Lond B"},{"key":"2760_CR66","first-page":"906","volume":"15","author":"RF Margolskee","year":"1993","unstructured":"Margolskee RF, McHendry-Rinde B, Horn R: Panning transfected cells for electrophysiological studies. Biotechniques. 1993, 15: 906-911.","journal-title":"Biotechniques"},{"key":"2760_CR67","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1124\/mol.106.030809","volume":"71","author":"GT Young","year":"2007","unstructured":"Young GT, Broad LM, Zwart R, Astles PC, Bodkin M, Sher E, Millar NS: Species selectivity of a nicotinic acetylcholine receptor agonist is conferred by two adjacent extracellular \u03b24 amino acids that are implicated in the coupling of binding to channel gating. Mol Pharmacol. 2007, 71: 389-397.","journal-title":"Mol Pharmacol"}],"container-title":["BMC Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2202-13-73.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T19:42:29Z","timestamp":1630525349000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcneurosci.biomedcentral.com\/articles\/10.1186\/1471-2202-13-73"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,22]]},"references-count":67,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["2760"],"URL":"https:\/\/doi.org\/10.1186\/1471-2202-13-73","relation":{},"ISSN":["1471-2202"],"issn-type":[{"value":"1471-2202","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,22]]},"assertion":[{"value":"19 January 2012","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 May 2012","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 June 2012","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"73"}}