{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:01:07Z","timestamp":1791237667079,"version":"4.1.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Neurosci"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Rag1<\/jats:italic> (Recombination activation gene-1) mediates genomic rearrangement and is essential for adaptive immunity in vertebrates. This gene is also expressed in the olfactory epithelium, but its function there is unknown.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Using a transgenic zebrafish line and immunofluorescence, we show that <jats:italic>Rag1<\/jats:italic> is expressed and translated in a subset of olfactory sensory neurons (OSNs). Neurons expressing GFP under the <jats:italic>Rag1<\/jats:italic> promoter project their axons to the lateral region of the olfactory bulb only, and axons with the highest levels of GFP terminate in a single glomerular structure. A subset of GFP-expressing neurons contain G\u03b1<jats:sub>o<\/jats:sub>, a marker for microvillous neurons. None of the GFP-positive neurons express G\u03b1<jats:sub>olf<\/jats:sub>, G\u03b1<jats:sub>q<\/jats:sub> or the olfactory marker protein OMP. Depletion of RAG1, by morpholino-mediated knockdown or mutation, did not affect axon targeting. Calcium imaging indicates that amino acids evoke chemotopically organized glomerular activity patterns in a <jats:italic>Rag1<\/jats:italic> mutant.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>\n              <jats:italic>Rag1<\/jats:italic> expression is restricted to a subpopulation of zebrafish olfactory neurons projecting to the lateral olfactory bulb. RAG1 catalytic activity is not essential for axon targeting, nor is it likely to be required for regulation of odorant receptor expression or the response of OSNs to amino acids.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2202-6-46","type":"journal-article","created":{"date-parts":[[2005,7,15]],"date-time":"2005-07-15T18:13:51Z","timestamp":1121451231000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The recombination activation gene 1 (Rag1) is expressed in a subset of zebrafish olfactory neurons but is not essential for axon targeting or amino acid detection"],"prefix":"10.1186","volume":"6","author":[{"given":"Bo","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarada","family":"Bulchand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emre","family":"Yaksi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer W","family":"Friedrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Jesuthasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2005,7,15]]},"reference":[{"key":"180_CR1","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1016\/S0092-8674(94)90562-2","volume":"78","author":"A Chess","year":"1994","unstructured":"Chess A, Simon I, Cedar H, Axel R: Allelic inactivation regulates olfactory receptor gene expression. 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