{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T08:13:00Z","timestamp":1784967180994,"version":"3.55.0"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Pharmacol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Serotonin (5-hydroxytryptamine; 5-HT) delivered over 1\u2009week results in a sustained fall in blood pressure in the sham and deoxycorticosterone acetate (DOCA)-salt rat. We hypothesized 5-HT lowers blood pressure through direct receptor-mediated vascular relaxation. <jats:italic>In vivo<\/jats:italic>, 5-HT reduced mean arterial pressure (MAP), increased heart rate, stroke volume, cardiac index, and reduced total peripheral resistance during a 1\u2009week infusion of 5-HT (25 \u00b5g\/kg\/min) in the normotensive Sprague Dawley rat. The mesenteric vasculature was chosen as an ideal candidate for the site of 5-HT receptor mediated vascular relaxation given the high percentage of cardiac output the site receives. Real-time RT-PCR demonstrated that mRNA transcripts for the 5-HT<jats:sub>2B<\/jats:sub>, 5-HT<jats:sub>1B<\/jats:sub>, and 5-HT<jats:sub>7<\/jats:sub> receptors are present in sham and DOCA-salt superior mesenteric arteries. Immunohistochemistry and Western blot validated the presence of the 5-HT<jats:sub>2B<\/jats:sub>, 5- HT<jats:sub>1B<\/jats:sub> and 5-HT<jats:sub>7<\/jats:sub> receptor protein in sham and DOCA-salt superior mesenteric artery. Isometric contractile force was measured in endothelium-intact superior mesenteric artery and mesenteric resistance arteries in which the contractile 5- HT<jats:sub>2A<\/jats:sub> receptor was antagonized. Maximum concentrations of BW-723C86 (5- HT<jats:sub>2B<\/jats:sub> agonist), CP 93129 (5-HT<jats:sub>1B<\/jats:sub> agonist) or LP-44 (5-HT<jats:sub>7<\/jats:sub> agonist) did not relax the superior mesenteric artery from DOCA-salt rats <jats:italic>vs.<\/jats:italic> vehicle. Additionally, 5-HT (10<jats:sup>\u20139<\/jats:sup>\u2009M to 10<jats:sup>\u20135<\/jats:sup>\u2009M) did not cause relaxation in either contracted mesenteric resistance arteries or superior mesenteric arteries from normotensive Sprague- Dawley rats. Thus, although 5-HT receptors known to mediate vascular relaxation are present in the superior mesenteric artery, they are not functional, and are therefore not likely involved in a 5-HT-induced fall in total peripheral resistance and MAP.<\/jats:p>","DOI":"10.1186\/1471-2210-12-4","type":"journal-article","created":{"date-parts":[[2012,5,6]],"date-time":"2012-05-06T20:14:40Z","timestamp":1336335280000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["5-hydroxytryptamine (5-HT) reduces total peripheral resistance during chronic infusion: direct arterial mesenteric relaxation is not involved"],"prefix":"10.1186","volume":"12","author":[{"given":"Robert Patrick","family":"Davis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"Pattison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice M","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruslan","family":"Tiniakov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karie E","family":"Scrogin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie W","family":"Watts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,5,6]]},"reference":[{"key":"970_CR1","doi-asserted-by":"publisher","first-page":"800","DOI":"10.1038\/169800b0","volume":"169","author":"V Erspamer","year":"1952","unstructured":"Erspamer V, Aerso B: Identification of enteramine, the specific hormone of the enterochromaffin cell system, as 5-hydroxytryptamine. 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