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The esophagus dilation is known as chagasic megaesophagus (CM) and one of the severe late consequences of CM is the increased risk for esophageal carcinoma (ESCC). Based on the association between CM and ESCC, we investigated whether genes frequently showing unbalanced copy numbers in ESCC were altered in CM by fluorescence in situ (FISH) technology.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>A total of 50 formalin-fixed, paraffin-embedded esophageal mucosa specimens (40 from Chagas megaesophagus-CM, and 10 normal esophageal mucosa-NM) were analyzed. DNA FISH probes were tested for <jats:italic>FHIT<\/jats:italic>, <jats:italic>TP63<\/jats:italic>, <jats:italic>PIK3CA<\/jats:italic>, <jats:italic>EGFR, FGFR1<\/jats:italic>, <jats:italic>MYC<\/jats:italic>, <jats:italic>CDKN2A, YES1<\/jats:italic> and <jats:italic>NCOA3<\/jats:italic> genes, and centromeric sequences from chromosomes 3, 7 and 9.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>No differences between superficial and basal layers of the epithelial mucosa were found, except for loss of copy number of <jats:italic>EGFR<\/jats:italic> in the esophageal basal layer of CM group. Mean copy number of <jats:italic>CDKN2A and<\/jats:italic> CEP9 and frequency of nuclei with loss of <jats:italic>PIK3CA<\/jats:italic> were significantly different in the CM group compared with normal mucosa and marginal levels of deletions in <jats:italic>TP63<\/jats:italic>, <jats:italic>FHIT, PIK3CA, EGFR, CDKN2A, YES<\/jats:italic> and gains at <jats:italic>PIK3CA, TP63, FGFR1, MYC, CDNK2A<\/jats:italic> and <jats:italic>NCOA3<\/jats:italic> were detected in few CM cases, mainly with dilation grades III and IV. All changes occurred at very low levels.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Genomic imbalances common in esophageal carcinomas are not present in chagasic megaesophagus suggesting that these features will not be effective markers for risk assessment of ESCC in patients with chagasic megaesophagus.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-230x-10-20","type":"journal-article","created":{"date-parts":[[2010,2,17]],"date-time":"2010-02-17T19:15:47Z","timestamp":1266434147000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Chromosomal imbalances are uncommon in chagasic megaesophagus"],"prefix":"10.1186","volume":"10","author":[{"given":"Marilanda F","family":"Bellini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio J","family":"Manzato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana E","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marileila","family":"Varella-Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,2,17]]},"reference":[{"key":"411_CR1","unstructured":"WHO, World Health Organization. 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