{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:16:55Z","timestamp":1758637015629},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2005,1,18]],"date-time":"2005-01-18T00:00:00Z","timestamp":1106006400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"},{"start":{"date-parts":[[2005,1,18]],"date-time":"2005-01-18T00:00:00Z","timestamp":1106006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Hematol"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                        <jats:title>Background<\/jats:title>\n                        <jats:p>With chronic infection, hepatitis C virus (HCV) RNA can be detected in B cells and associated with B-cell disorders, but these are not well defined.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Methods<\/jats:title>\n                        <jats:p>The relationship between HCV infection and lymphocyte subpopulations was evaluated rigorously in 120 asymptomatic hemophilic patients, randomly selected from a prospective cohort study. CD4<jats:sup>+<\/jats:sup> T cells, CD8<jats:sup>+<\/jats:sup> T cells, CD19<jats:sup>+<\/jats:sup> B cells, and CD56<jats:sup>+<\/jats:sup> NK cells were quantified by flow cytometry using cryopreserved peripheral blood mononuclear cells of 24 hemophilic patients in each of five age-matched groups [uninfected; chronic HCV with or without human immunodeficiency virus (HIV); and cleared HCV with or without HIV].<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Results<\/jats:title>\n                        <jats:p>As expected, patients with HIV had significantly reduced CD4<jats:sup>+<\/jats:sup> and increased CD8<jats:sup>+<\/jats:sup> T cells. Irrespective of HIV, patients with chronic HCV infection had approximately 25% fewer CD19<jats:sup>+<\/jats:sup> B cells than those without chronic HCV infection.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Conclusions<\/jats:title>\n                        <jats:p>These data support the hypothesis that asymptomatic patients with chronic HCV infection have an altered B-lymphocyte population.<\/jats:p>\n                     <\/jats:sec>","DOI":"10.1186\/1471-2326-5-2","type":"journal-article","created":{"date-parts":[[2005,1,18]],"date-time":"2005-01-18T17:24:53Z","timestamp":1106069093000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection: a nested cross-sectional study"],"prefix":"10.1186","volume":"5","author":[{"given":"Mingdong","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas R","family":"O'Brien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C","family":"Kopp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James J","family":"Goedert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"for the Multicenter Hemophilia Cohort Study","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2005,1,18]]},"reference":[{"key":"18_CR1","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1016\/0168-8278(92)90073-X","volume":"15","author":"AL Zignego","year":"1992","unstructured":"Zignego AL, Macchia D, Monti M, Thiers V, Mazzetti M, Foschi M, Maggi E, Romagnani S, Gentilini P, Brechot C: Infection of peripheral mononuclear blood cells by hepatitis C virus. 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