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PCR assays (psaA, LytA, ply, spn9802-PCR) were previously described for the specific amplification of <jats:italic>S. pneumoniae<\/jats:italic>, but <jats:italic>psaA<\/jats:italic>-PCR was the only one found not to cross-react with <jats:italic>S. pseudopneumoniae<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Spne-PCR, developed for this study, and psaA-PCR were the only two assays which did not mis-identify <jats:italic>S. pseudopneumoniae<\/jats:italic> as <jats:italic>S. pneumoniae<\/jats:italic>. Four other PCR assays and the AccuProbe assay were unable to distinguish between these species.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2334-10-104","type":"journal-article","created":{"date-parts":[[2010,5,25]],"date-time":"2010-05-25T06:15:23Z","timestamp":1274768123000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays"],"prefix":"10.1186","volume":"10","author":[{"given":"Nabil Abdullah","family":"El Aila","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Emler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarja","family":"Kaijalainen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"De Baere","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart","family":"Saerens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elife","family":"Alkan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter","family":"Deschaght","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Verhelst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Vaneechoutte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,4,29]]},"reference":[{"issue":"6","key":"1084_CR1","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1055\/s-2005-925523","volume":"26","author":"A Ortqvist","year":"2005","unstructured":"Ortqvist A, Hedlund J, Kalin M: Streptococcus pneumoniae: epidemiology, risk factors, and clinical features. 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