{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T02:02:43Z","timestamp":1793325763723,"version":"4.5.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2010,9,29]],"date-time":"2010-09-29T00:00:00Z","timestamp":1285718400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Streptococcus agalactiae<\/jats:italic> (group B streptococcus; GBS) is a significant cause of perinatal and neonatal infections worldwide. To detect GBS colonization in pregnant women, the CDC recommends isolation of the bacterium from vaginal and anorectal swab samples by growth in a selective enrichment medium, such as Lim broth (Todd-Hewitt broth supplemented with selective antibiotics), followed by subculture on sheep blood agar. However, this procedure may require 48 h to complete. We compared different sampling and culture techniques for the detection of GBS.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>A total of 300 swabs was taken from 100 pregnant women at 35-37 weeks of gestation. For each subject, one rectovaginal, one vaginal and one rectal ESwab were collected. Plating onto Columbia CNA agar (CNA), group B streptococcus differential agar (GBSDA) (Granada Medium) and chromID Strepto B agar (CA), with and without Lim broth enrichment, were compared. The isolates were confirmed as S. agalactiae using the CAMP test on blood agar and by molecular identification with tDNA-PCR or by 16S rRNA gene sequence determination.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The overall GBS colonization rate was 22%. GBS positivity for rectovaginal sampling (100%) was significantly higher than detection on the basis of vaginal sampling (50%), but not significantly higher than for rectal sampling (82%). Direct plating of the rectovaginal swab on CNA, GBSDA and CA resulted in detection of 59, 91 and 95% of the carriers, respectively, whereas subculturing of Lim broth yielded 77, 95 and 100% positivity, respectively. Lim broth enrichment enabled the detection of only one additional GBS positive subject. There was no significant difference between GBSDA and CA, whereas both were more sensitive than CNA. Direct culture onto GBSDA or CA (91 and 95%) detected more carriers than Lim broth enrichment and subculture onto CNA (77%). One false negative isolate was observed on GBSDA, and three false positives on CA.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>In conclusion, rectovaginal sampling increased the number GBS positive women detected, compared to vaginal and\/or rectal sampling. Direct plating on CA and\/or GBSDA provided rapid detection of GBS that was at least as sensitive and specific as the CDC recommended method of Lim broth subcultured onto non chromogenic agar.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2334-10-285","type":"journal-article","created":{"date-parts":[[2010,9,29]],"date-time":"2010-09-29T06:17:05Z","timestamp":1285741025000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":100,"title":["Comparison of different sampling techniques and of different culture methods for detection of group B streptococcus carriage in pregnant women"],"prefix":"10.1186","volume":"10","author":[{"given":"Nabil A","family":"El Aila","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge","family":"Tency","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geert","family":"Claeys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"Saerens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piet","family":"Cools","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Verstraelen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marleen","family":"Temmerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Verhelst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Vaneechoutte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,9,29]]},"reference":[{"issue":"5","key":"1265_CR1","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1093\/infdis\/137.5.524","volume":"137","author":"BF Anthony","year":"1978","unstructured":"Anthony BF, Okada DM, Hobel CJ: Epidemiology of group B streptococcus: longitudinal observations during pregnancy. J Infect Dis. 1978, 137 (5): 524-530.","journal-title":"J Infect Dis"},{"issue":"5","key":"1265_CR2","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1093\/infdis\/148.5.810","volume":"148","author":"KM Boyer","year":"1983","unstructured":"Boyer KM, Gadzala CA, Kelly PD, Gotoff SP: Selective intrapartum chemoprophylaxis of neonatal group B streptococcal early-onset disease. III. Interruption of mother-to-infant transmission. J Infect Dis. 1983, 148 (5): 810-816.","journal-title":"J Infect Dis"},{"issue":"6","key":"1265_CR3","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1093\/infdis\/145.6.794","volume":"145","author":"HC Dillon Jr","year":"1982","unstructured":"Dillon HC, Gray E, Pass MA, Gray BM: Anorectal and vaginal carriage of group B streptococci during pregnancy. J Infect Dis. 1982, 145 (6): 794-799.","journal-title":"J Infect Dis"},{"key":"1265_CR4","first-page":"1","volume":"45","author":"Centers for Disease Control and Prevention","year":"1996","unstructured":"Centers for Disease Control and Prevention: Prevention of perinatal group B streptococcal disease: a public health perspective. Morbid Mortal Weekly Rep. 1996, 45: 1-24.","journal-title":"Morbid Mortal Weekly Rep"},{"key":"1265_CR5","unstructured":"Prevention of perinatal group B streptococcal infections. 2003, Guidelines from the Belgian Health Council, (SHC.7721), [http:\/\/www.health.fgov.be\/CSH_HGR\/English\/Brochures\/GBS_EN2003.pdf]"},{"issue":"4","key":"1265_CR6","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/S1084-2756(02)90117-X","volume":"7","author":"WE Benitz","year":"2002","unstructured":"Benitz WE: Perinatal treatment to prevent early onset group B streptococcal sepsis. Semin Neonatol. 2002, 7 (4): 301-314.","journal-title":"Semin Neonatol"},{"key":"1265_CR7","first-page":"1","volume":"51","author":"Centers for Disease Control and Prevention","year":"2002","unstructured":"Centers for Disease Control and Prevention: Prevention of perinatal group B streptococcal disease. Morbid Mortal Weekly Rep. 2002, 51: 1-22.","journal-title":"Morbid Mortal Weekly Rep"},{"issue":"8","key":"1265_CR8","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1128\/JCM.36.8.2298-2300.1998","volume":"36","author":"WM Dunne Jr","year":"1998","unstructured":"Dunne WM, Holland-Staley CA: Comparison of NNA agar culture and selective broth culture for detection of group B streptococcal colonization in women. J Clin Microbiol. 1998, 36 (8): 2298-2300.","journal-title":"J Clin Microbiol"},{"issue":"1","key":"1265_CR9","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1186\/1471-2334-9-153","volume":"9","author":"NA El Aila","year":"2009","unstructured":"El Aila NA, Tency I, Claeys G, Saerens B, Verhelst R, De Backer E, Temmerman M, Vaneechoutte M: Genotyping of Streptococcus agalactiae (group B streptococci) isolated from vaginal and rectal swabs of women at 35-37 weeks of pregnancy. BMC Infect Dis. 2009, 9 (1): 153-10.1186\/1471-2334-9-153.","journal-title":"BMC Infect Dis"},{"issue":"3","key":"1265_CR10","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/0029-7844(94)00412-7","volume":"85","author":"EH Philipson","year":"1995","unstructured":"Philipson EH, Palermino DA, Robinson A: Enhanced antenatal detection of group B streptococcus colonization. Obstet Gynecol. 1995, 85 (3): 437-439. 10.1016\/0029-7844(94)00412-7.","journal-title":"Obstet Gynecol"},{"issue":"2","key":"1265_CR11","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1007\/s100960000446","volume":"20","author":"M Votava","year":"2001","unstructured":"Votava M, Tejkalova M, Drabkova M, Unzeitig V, Braveny I: Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor. Eur J Clin Microbiol Infect Dis. 2001, 20 (2): 120-122. 10.1007\/s100960000446.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"5","key":"1265_CR12","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1128\/JCM.02047-07","volume":"46","author":"KG Van Horn","year":"2008","unstructured":"Van Horn KG, Audette CD, Sebeck D, Tucker KA: Comparison of the Copan ESwab system with two Amies agar swab transport systems for maintenance of microorganism viability. J Clin Microbiol. 2008, 46 (5): 1655-1658. 10.1128\/JCM.02047-07.","journal-title":"J Clin Microbiol"},{"issue":"4","key":"1265_CR13","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1007\/s10096-010-0883-5","volume":"29","author":"S Nys","year":"2010","unstructured":"Nys S, Vijgen S, Magerman K, Cartuyvels R: Comparison of Copan eSwab with the Copan Venturi Transystem for the quantitative survival of Escherichia coli, Streptococcus agalactiae and Candida albicans. Eur J Clin Microbiol Infect Dis. 2010, 29 (4): 453-456. 10.1007\/s10096-010-0883-5.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"4","key":"1265_CR14","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1056\/NEJMoa020205","volume":"347","author":"SJ Schrag","year":"2002","unstructured":"Schrag SJ, Zell ER, Lynfield R, Roome A, Arnold KE, Craig AS, Harrison LH, Reingold A, Stefonek K, Smith G, et al: A population-based comparison of strategies to prevent early-onset group B streptococcal disease in neonates. N Engl J Med. 2002, 347 (4): 233-239. 10.1056\/NEJMoa020205.","journal-title":"N Engl J Med"},{"issue":"4","key":"1265_CR15","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1128\/jcm.30.4.1019-1021.1992","volume":"30","author":"M de la Rosa","year":"1992","unstructured":"de la Rosa M, Perez M, Carazo C, Pareja L, Peis JI, Hernandez F: New Granada Medium for detection and identification of group B streptococci. J Clin Microbiol. 1992, 30 (4): 1019-1021.","journal-title":"J Clin Microbiol"},{"issue":"8005","key":"1265_CR16","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1016\/S0140-6736(77)91055-8","volume":"1","author":"AK Islam","year":"1977","unstructured":"Islam AK: Rapid recognition of group B streptococci. Lancet. 1977, 1 (8005): 256-257. 10.1016\/S0140-6736(77)91055-8.","journal-title":"Lancet"},{"issue":"9","key":"1265_CR17","doi-asserted-by":"publisher","first-page":"6367","DOI":"10.1128\/AEM.00756-06","volume":"72","author":"M Rosa-Fraile","year":"2006","unstructured":"Rosa-Fraile M, Rodriguez-Granger J, Haidour-Benamin A, Cuerva JM, Sampedro A: Granadaene: proposed structure of the group B streptococcus polyenic pigment. Appl Environ Microbiol. 2006, 72 (9): 6367-6370. 10.1128\/AEM.00756-06.","journal-title":"Appl Environ Microbiol"},{"issue":"3","key":"1265_CR18","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.mimet.2008.02.024","volume":"73","author":"A Tazi","year":"2008","unstructured":"Tazi A, Reglier-Poupet H, Dautezac F, Raymond J, Poyart C: Comparative evaluation of Strepto B ID chromogenic medium and Granada media for the detection of group B streptococcus from vaginal samples of pregnant women. J Microbiol Methods. 2008, 73 (3): 263-265. 10.1016\/j.mimet.2008.02.024.","journal-title":"J Microbiol Methods"},{"issue":"3","key":"1265_CR19","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.patbio.2008.09.002","volume":"57","author":"A Tazi","year":"2009","unstructured":"Tazi A, Doloy A, Reglier-Poupet H, Hemet ME, Raymond J, Poyart C: Evaluation of the new chromogenic medium StrepB Select for screening of group B streptococcus in pregnant women. Pathol Biol (Paris). 2009, 57 (3): 225-228.","journal-title":"Pathol Biol (Paris)"},{"issue":"6","key":"1265_CR20","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1111\/j.1472-765X.2006.02023.x","volume":"43","author":"JD Perry","year":"2006","unstructured":"Perry JD, Oliver M, Nicholson A, Wright J, Gould FK: Evaluation of a new chromogenic agar medium for isolation and identification of group B streptococci. Lett Appl Microbiol. 2006, 43 (6): 615-618. 10.1111\/j.1472-765X.2006.02023.x.","journal-title":"Lett Appl Microbiol"},{"issue":"4","key":"1265_CR21","doi-asserted-by":"publisher","first-page":"1436","DOI":"10.1128\/JCM.39.4.1436-1442.2001","volume":"39","author":"M Baele","year":"2001","unstructured":"Baele M, Storms V, Haesebrouck F, Devriese LA, Gillis M, Verschraegen G, de Baere T, Vaneechoutte M: Application and evaluation of the interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) for identification of streptococcus species. J Clin Microbiol. 2001, 39 (4): 1436-1442. 10.1128\/JCM.39.4.1436-1442.2001.","journal-title":"J Clin Microbiol"},{"issue":"4","key":"1265_CR22","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/j.diagmicrobio.2008.03.007","volume":"61","author":"D Smith","year":"2008","unstructured":"Smith D, Perry JD, Laine L, Galloway A, Gould FK: Comparison of BD GeneOhm real-time polymerase chain reaction with chromogenic and conventional culture methods for detection of group B streptococcus in clinical samples. Diagn Micr Infec Dis. 2008, 61 (4): 369-372. 10.1016\/j.diagmicrobio.2008.03.007.","journal-title":"Diagn Micr Infec Dis"},{"issue":"3","key":"1265_CR23","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.mimet.2010.06.008","volume":"82","author":"DM Poisson","year":"2010","unstructured":"Poisson DM, Chandemerle M, Guinard J, Evrard ML, Naydenova D, Mesnard L: Evaluation of CHROMagar StrepB: A new chromogenic agar medium for aerobic detection of group B streptococci in perinatal samples. J Microbiol methods. 2010, 82 (3): 238-242. 10.1016\/j.mimet.2010.06.008.","journal-title":"J Microbiol methods"},{"issue":"5","key":"1265_CR24","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1007\/BF02962249","volume":"26","author":"TA Madani","year":"1998","unstructured":"Madani TA, Harding GK, Helewa M, Alfa MJ: Screening pregnant women for group B streptococcal colonization. Infection. 1998, 26 (5): 288-291. 10.1007\/BF02962249.","journal-title":"Infection"},{"issue":"5","key":"1265_CR25","first-page":"447","volume":"49","author":"JD Quinlan","year":"2000","unstructured":"Quinlan JD, Hill DA, Maxwell BD, Boone S, Hoover F, Lense JJ: The necessity of both anorectal and vaginal cultures for group B streptococcus screening during pregnancy. J Fam Pract. 2000, 49 (5): 447-448.","journal-title":"J Fam Pract"},{"issue":"11","key":"1265_CR26","first-page":"1659","volume":"149","author":"UD Allen","year":"1993","unstructured":"Allen UD, Navas L, King SM: Effectiveness of intrapartum penicillin prophylaxis in preventing early-onset group B streptococcal infection: results of a meta-analysis. CMAJ. 1993, 149 (11): 1659-1665.","journal-title":"CMAJ"},{"issue":"2","key":"1265_CR27","doi-asserted-by":"publisher","first-page":"108","DOI":"10.4067\/S0716-10182008000200003","volume":"25","author":"TM Diaz","year":"2008","unstructured":"Diaz TM, Nieves BM: Comparison between culture media and procedures to detect Streptococcus agalactiae in pregnant women. Rev Chil Infectol. 2008, 25 (2): 108-113. 10.4067\/S0716-10182008000200003.","journal-title":"Rev Chil Infectol"},{"issue":"2","key":"1265_CR28","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/0732-8893(95)00022-3","volume":"21","author":"MW Platt","year":"1995","unstructured":"Platt MW, McLaughlin JC, Gilson GJ, Wellhoner MF, Nims LJ: Increased recovery of group B streptococcus by the inclusion of rectal culturing and enrichment. Diagn Microbiol Infect Dis. 1995, 21 (2): 65-68. 10.1016\/0732-8893(95)00022-3.","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"4","key":"1265_CR29","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1590\/S1413-86702006000400006","volume":"10","author":"ML Nomura","year":"2006","unstructured":"Nomura ML, Passini Junior R, Oliveira UM: Selective versus non-selective culture medium for group B streptococcus detection in pregnancies complicated by preterm labor or preterm-premature rupture of membranes. Braz J Infect Dis. 2006, 10 (4): 247-250. 10.1590\/S1413-86702006000400006.","journal-title":"Braz J Infect Dis"},{"issue":"9","key":"1265_CR30","doi-asserted-by":"publisher","first-page":"3975","DOI":"10.1128\/JCM.42.9.3975-3977.2004","volume":"42","author":"C Gupta","year":"2004","unstructured":"Gupta C, Briski LE: Comparison of two culture media and three sampling techniques for sensitive and rapid screening of vaginal colonization by group B streptococcus in pregnant women. J Clin Microbiol. 2004, 42 (9): 3975-3977. 10.1128\/JCM.42.9.3975-3977.2004.","journal-title":"J Clin Microbiol"},{"issue":"4","key":"1265_CR31","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1128\/jcm.18.4.779-785.1983","volume":"18","author":"M De La Rosa","year":"1983","unstructured":"De La Rosa M, Villareal R, Vega D, Miranda C, Martinezbrocal A: Granada medium for detection and identification of group B streptococci. J Clin Microbiol. 1983, 18 (4): 779-785.","journal-title":"J Clin Microbiol"},{"issue":"8","key":"1265_CR32","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1111\/j.1469-0691.2005.01195.x","volume":"11","author":"G Bou","year":"2005","unstructured":"Bou G, Figueira M, Canle D, Cartelle M, Eiros JM, Villanueva R: Evaluation of group B streptococcus Differential Agar for detection and isolation of Streptococcus agalactiae. Clin Microbiol Infect. 2005, 11 (8): 676-678. 10.1111\/j.1469-0691.2005.01195.x.","journal-title":"Clin Microbiol Infect"},{"issue":"12","key":"1265_CR33","doi-asserted-by":"publisher","first-page":"1487","DOI":"10.1007\/s10096-009-0811-8","volume":"28","author":"T Regnath","year":"2009","unstructured":"Regnath T, Ignatius R: High stability of a new Granada medium agar that allows rapid and accurate detection of colonization with group B streptococci in pregnant women. Eur J Clin Microbiol Infect Dis. 2009, 28 (12): 1487-1489. 10.1007\/s10096-009-0811-8.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"3","key":"1265_CR34","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1007\/s10096-007-0421-2","volume":"27","author":"A Adler","year":"2008","unstructured":"Adler A, Block C, Engelstein D, Hochner-Celnikcier D, Drai-Hassid R, Moses AE: Culture-based methods for detection and identification of Streptococcus agalactiae in pregnant women - what are we missing?. Eur J Clin Microbiol Infect Dis. 2008, 27 (3): 241-243. 10.1007\/s10096-007-0421-2.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"1","key":"1265_CR35","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1046\/j.1469-0691.2001.00156.x","volume":"7","author":"G Claeys","year":"2001","unstructured":"Claeys G, Verschraegen G, Temmerman M: Modified Granada Agar Medium for the detection of group B streptococcus carriage in pregnant women. Clin Microbiol Infect. 2001, 7 (1): 22-24. 10.1046\/j.1469-0691.2001.00156.x.","journal-title":"Clin Microbiol Infect"},{"issue":"11","key":"1265_CR36","doi-asserted-by":"publisher","first-page":"4329","DOI":"10.1128\/JCM.40.11.4329-4331.2002","volume":"40","author":"SB Overman","year":"2002","unstructured":"Overman SB, Eley DD, Jacobs BE, Ribes JA: Evaluation of methods to increase the sensitivity and timeliness of detection of Streptococcus agalactiae in pregnant women. J Clin Microbiol. 2002, 40 (11): 4329-4331. 10.1128\/JCM.40.11.4329-4331.2002.","journal-title":"J Clin Microbiol"},{"issue":"6","key":"1265_CR37","doi-asserted-by":"crossref","first-page":"718","DOI":"10.5858\/2003-127-718-CODSVN","volume":"127","author":"S Elsayed","year":"2003","unstructured":"Elsayed S, Gregson DB, Church DL: Comparison of direct selective versus nonselective agar media plus LIM broth enrichment for determination of group B streptococcus colonization status in pregnant women. Arch Pathol Lab Med. 2003, 127 (6): 718-720.","journal-title":"Arch Pathol Lab Med"},{"issue":"2","key":"1265_CR38","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1128\/JCM.43.2.896-897.2005","volume":"43","author":"JS Heelan","year":"2005","unstructured":"Heelan JS, Struminsky J, Lauro P, Sung CJ: Evaluation of a new selective enrichment broth for detection of group B streptococci in pregnant women. J Clin Microbiol. 2005, 43 (2): 896-897. 10.1128\/JCM.43.2.896-897.2005.","journal-title":"J Clin Microbiol"},{"issue":"8","key":"1265_CR39","doi-asserted-by":"crossref","first-page":"2674","DOI":"10.1128\/JCM.37.8.2674-2677.1999","volume":"37","author":"M Rosa-Fraile","year":"1999","unstructured":"Rosa-Fraile M, Rodriguez-Granger J, Cueto-Lopez M, Sampedro A, Gaye EB, Haro JM, Andreu A: Use of Granada medium to detect group B streptococcal colonization in pregnant women. J Clin Microbiol. 1999, 37 (8): 2674-2677.","journal-title":"J Clin Microbiol"},{"issue":"8","key":"1265_CR40","doi-asserted-by":"crossref","first-page":"2648","DOI":"10.1128\/JCM.37.8.2648-2651.1999","volume":"37","author":"EG Gil","year":"1999","unstructured":"Gil EG, Rodriguez MC, Bartolome R, Berjano B, Cabero L, Andreu A: Evaluation of the Granada agar plate for detection of vaginal and rectal group B streptococci in pregnant women. J Clin Microbiol. 1999, 37 (8): 2648-2651.","journal-title":"J Clin Microbiol"},{"issue":"10","key":"1265_CR41","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1007\/s10096-003-1002-7","volume":"22","author":"H Blanckaert","year":"2003","unstructured":"Blanckaert H, Frans J, Bosteels J, Hanssens M, Verhaegen J: Optimisation of prenatal group B streptococcal screening. Eur J Clin Microbiol Infect Dis. 2003, 22 (10): 619-621. 10.1007\/s10096-003-1002-7.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"1","key":"1265_CR42","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1128\/jcm.8.1.105-107.1978","volume":"8","author":"K Merritt","year":"1978","unstructured":"Merritt K, Jacobs NJ: Characterization and incidence of pigment production by human clinical group B streptococci. J Clin Microbiol. 1978, 8 (1): 105-107.","journal-title":"J Clin Microbiol"},{"issue":"10","key":"1265_CR43","doi-asserted-by":"crossref","first-page":"3212","DOI":"10.1128\/JB.181.10.3212-3219.1999","volume":"181","author":"B Spellerberg","year":"1999","unstructured":"Spellerberg B, Pohl B, Haase G, Martin S, Weber-Heynemann J, Lutticken R: Identification of genetic determinants for the hemolytic activity of Streptococcus agalactiae by ISS1 transposition. J Bacteriol. 1999, 181 (10): 3212-3219.","journal-title":"J Bacteriol"}],"container-title":["BMC Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2334-10-285.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1471-2334-10-285\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2334-10-285","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2334-10-285.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T04:09:52Z","timestamp":1630469392000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcinfectdis.biomedcentral.com\/articles\/10.1186\/1471-2334-10-285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,29]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["1265"],"URL":"https:\/\/doi.org\/10.1186\/1471-2334-10-285","relation":{},"ISSN":["1471-2334"],"issn-type":[{"value":"1471-2334","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,29]]},"assertion":[{"value":"17 August 2010","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2010","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2010","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"285"}}