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The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>A multi-species indirect immunosorbent assay (iELISA) using <jats:italic>Brucella<\/jats:italic> S-LPS antigen was developed. In several regions having brucellosis in livestock, individual serum samples were taken between 1999 and 2009 from 2,579 wild bovids, 6,448 wild cervids and4,454 Eurasian wild boar (<jats:italic>Sus scrofa<\/jats:italic>), and tested to assess brucellosis apparent prevalence. Strains isolated from wild boar were characterized to identify the presence of markers shared with the strains isolated from domestic pigs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Mean apparent prevalence below 0.5% was identified in chamois (<jats:italic>Rupicapra pyrenaica<\/jats:italic>), Iberian wild goat (<jats:italic>Capra pyrenaica<\/jats:italic>), and red deer (<jats:italic>Cervus elaphus<\/jats:italic>). Roe deer (<jats:italic>Capreolus capreolus<\/jats:italic>), fallow deer (<jats:italic>Dama dama<\/jats:italic>), mouflon (<jats:italic>Ovis aries<\/jats:italic>) and Barbary sheep (<jats:italic>Ammotragus lervia<\/jats:italic>) tested were seronegative. Only one red deer and one Iberian wild goat resulted positive in culture, isolating <jats:italic>B. abortus<\/jats:italic> biovar 1 and <jats:italic>B. melitensis<\/jats:italic> biovar 1, respectively. Apparent prevalence in wild boar ranged from 25% to 46% in the different regions studied, with the highest figures detected in South-Central Spain. The probability of wild boar being positive in the iELISA was also affected by age, age-by-sex interaction, sampling month, and the density of outdoor domestic pigs. A total of 104 bacterial isolates were obtained from wild boar, being all identified as <jats:italic>B. suis<\/jats:italic> biovar 2. DNA polymorphisms were similar to those found in domestic pigs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>In conclusion, brucellosis in wild boar is widespread in the Iberian Peninsula, thus representing an important threat for domestic pigs. By contrast, wild ruminants were not identified as a significant brucellosis reservoir for livestock.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2334-10-46","type":"journal-article","created":{"date-parts":[[2010,3,18]],"date-time":"2010-03-18T19:15:52Z","timestamp":1268939752000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":142,"title":["Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates"],"prefix":"10.1186","volume":"10","author":[{"given":"Pilar M","family":"Mu\u00f1oz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Boadella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maricruz","family":"Arnal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J","family":"de Miguel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Revilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaqu\u00edn","family":"Vicente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pelayo","family":"Acevedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1lvaro","family":"Oleaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Ruiz-Fons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara M","family":"Mar\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M","family":"Prieto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"de la Fuente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Barral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montserrat","family":"Barber\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Fern\u00e1ndez","family":"de Luco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M","family":"Blasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Gort\u00e1zar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,3,5]]},"reference":[{"key":"1026_CR1","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1111\/j.1365-2672.2005.02622.x","volume":"98","author":"SJ Cutler","year":"2005","unstructured":"Cutler SJ, Whatmore AM, Commander AJ: Brucellosis, new aspects of an old disease. 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